Curapath

The desired critical attributes for the dry powder product (DPP) in this formulation were accomplished by varying the excipients, lipid #composition, concentration of LNPs, and weight percentage of mRNA. Leucine alone or in combination with mannitol improved the formulation by increasing the mRNA yield as well as decreasing the particle size. #Intratracheal administration of the DPP in mice resulted in luciferase expression in the #trachea and #lungs indicating successful delivery of functional mRNA. https://lnkd.in/ePHNzc6U #mRNA #DPP #LNP #Lipid #Drugdelivery #Pulmonary #Engineering

At Curapath, our hearts go out to everyone affected by the recent floods. We extend our deepest condolences to the families of those who have lost loved ones, and we stand in solidarity with the communities facing this unprecedented disaster. We are incredibly grateful to the first responders, volunteers, and rescuers working tirelessly on the front lines. Meanwhile, our main operations remain functional as we continue to support our clients and partners during this challenging time. #CommunitySupport #Solidarity #WeCare

The #development of in vitro transcribed mRNA (IVT mRNA)-based therapeutics/vaccines depends on the management of IVT mRNA immunogenicity. IVT mRNA, which is used for intracellular protein translation, often triggers unwanted immune responses, interfering with protein expression and leading to reduced therapeutic #efficacy. Currently, the predominant approach for mitigating immune responses involves the incorporation of costly chemically modified #nucleotides like pseudouridine (Ψ) or N1-methylpseudouridine (m1Ψ) into IVT mRNA, raising concerns about expense and the potential misincorporation of amino acids into chemically modified codon sequences. https://lnkd.in/expyGtcb #mRNA #Expression #Immunogenicity #Vaccine #Engineering #Chemicalengineering #Invitro #Therapeutics

A greater understanding of vaccine delivery system dynamics and resulting protein expression in target tissues and specific immune cells over time could inform future mRNA vaccine design, because questions remain regarding the trafficking of mRNA formulated in lipid nanoparticles (mRNA-LNPs). https://lnkd.in/diWJpDEd #Vaccine #mRNA #Expression #Intramuscular #Drugdelivery #Engineering

We will be in Boston on November 12-14th for the 12th International #mRNA Health Conference! Reach out to Samuel Garcia Del Blanco to schedule a time to meet up. #innovation

This study encompasses engineering of the innate immune cells, NK cells, toward intrinsically secreting interleukin-2 (IL-2), a critical #cytokine that amplifies immune responses by employing a #lipid #nanoparticle (LNP)-enabled IL-2 #mRNA delivery. This innovative approach not only enhances the efficacy of NK cells but also opens new avenues for improved immune therapy, potentially revolutionizing the #treatment of various cancers. https://lnkd.in/ehu7kPri #NK #LNP #Transfection #Precision #Cancer #Engineering

💊 The Booming Topical Drug Delivery Market 💉 📥 Download the PDF: https://lnkd.in/dPfxbKtG The global topical drug delivery market is poised for major growth, projected to reach $317.8 billion by 2027 at a CAGR of 8.9%. Key Drivers: 🩹 High incidence of burn injuries globally 💨 Increasing number of smokers worldwide ⬆️ Growing prevalence of diabetes 🔬 Advancements in transdermal drug delivery systems Major Opportunities: 🏡 Rising demand for self-administration and home care, especially for the elderly ⌛ Transdermal patches enable painless, sustained drug delivery North America currently dominates the market, fueled by high skin disease prevalence, preference for painless delivery, and demand for self-administered drugs. 𝐓𝐡𝐞 𝐜𝐨𝐦𝐩𝐚𝐧𝐢𝐞𝐬 𝐟𝐞𝐚𝐭𝐮𝐫𝐞𝐝 𝐢𝐧 𝐭𝐡𝐢𝐬 𝐫𝐞𝐩𝐨𝐫𝐭 𝐢𝐧𝐜𝐥𝐮𝐝𝐞: Glenmark Pharmaceuticals, Galderma, Johnson & Johnson, GlaxoSmithKline Consumer Healthcare, L.P., Bausch Health Companies Inc., Hisamitsu Pharmaceutical Co., Inc., Cipla, Bayer, Viatris, 3M, Merck & Co., Inc., Crescita Therapeutics, Novartis, Boehringer Ingelheim, Pfizer, Teva Pharmaceuticals, Bristol Myers Squibb, Lead Chemicals , Purdue Pharma L.P., Lavipharm, AbbVie, CMP Pharma, Inc., ENCORE DERMATOLOGY, INC., ProSolus, Inc., and Rusan Pharma Ltd, and many more. Other Companies that are Operating in this Market Globally: Slayback Pharma LGM Pharma ARx, LLC Tapemark Noble, An Aptar Pharma Company Donatelle Trustlife Labs Drug R&D Center PYC Therapeutics (ASX: PYC) ProMed Molded Products Curapath Starpharma BePharBel Manufacturing SA Alivia Health University of Washington Bioengineering Química del Nalón The topical drug delivery landscape is ripe with innovation potential to improve patient accessibility, compliance, and outcomes. Keep watching this space! #TopicalDrugDelivery #Pharma #Transdermal #Dermatology #MedicalDevices

As one of the critical attributes, understanding #mRNA lipid nanoparticle (#LNP) stability is critical in product development. However, the correlation between LNPs’ physiochemical #characteristics and their #potency still remains unclear. The lack of regulatory guidance on the specifications for mRNA-LNPs is also partially due to this underexplored relationship. This study outlines a three-month #stability study of heat-stressed mRNA-LNP samples. The mRNA-LNP samples were analyzed for their mRNA #degradation, LNP particle sizes, and mRNA #encapsulation #efficiency. In vitro cell potency was also evaluated and correlated with these above-mentioned physiochemical characterizations. Follow the link to the article to read more. https://lnkd.in/dRi2kH-Z #Nanoparticle #LipidNanoparticle #Stability #Invitro #Vaccine #Engineering #StabilityStudies #PhysicalChemistry #Chemistry #InVitro #Studies #Research

The #immunesystem is an especially appealing target due to its involvement in many different diseases, and ex vivo-engineered immune cell therapies like chimeric antigen receptor (#CAR) T therapy have already demonstrated remarkable clinical success in certain blood cancers. Although gene delivery can potentially address some of the cost and manufacturing concerns associated with current autologous immune cell therapies, transfecting immune cells in vivo is challenging. https://lnkd.in/e26VRQmJ #Nanoparticle #Lipid #Genedelivery #Engineering #InVivo #Transfection #CART #immunotherapy #immunology #cancer #cancerresearch #research #genetherapy #geneediting #LNP #PNP #polymers

Lipid nanoparticles (#LNPs) employing ionizable lipids are the most advanced technology for delivery of RNA, most notably mRNA, to cells. LNPs represent well-defined core–shell particles with efficient nucleic acid encapsulation, low #immunogenicity and enhanced efficacy. While much is known about the structure and activity of LNPs, less attention is given to the timing of LNP #uptake, #cytosolic transfer and protein expression. However, LNP #kinetics is a key factor determining delivery efficiency. Hence quantitative insight into the multi-cascaded pathway of LNPs is of interest to elucidate the mechanism of delivery. https://lnkd.in/etjCH_ix #mRNA #RNA #LNP #Drugdelivery #Expression #Engineering #ChemicalEngineering #Innovation #MolecularBiology #Chemistry