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Biobulletins reposted this
#Insulators are a class of regulatory DNA elements that possess a common ability to protect genes from inappropriate signals coming from their surrounding environment. Incorporating #tDNAinsulators into your construct could help you achieve superior yield. https://lnkd.in/g9Hmicmp
The #fluorescence microscopes are an integral part of #cellbiology labs these days with their applications ranging from localization of #proteins to determination of intracellular ion uptake. Lets learn a little about the innerworkings. https://lnkd.in/gpJtf3A4
Biobulletins reposted this
#CAP-T cells are emerging as an alternative to #CHO and #HEK cells for protein expression considering some inherent genetic aberrations in the latter ones, hindering expression of certain classes of proteins. Dive in for an introduction to these amazing cells! #CAP-T cells are a modification of #CEVEC's Aminocyte Production (CAP) cell line by transfecting them with E1 and pIX functions of #Adenovirus 5. They also constitutively expresses #SV40 large T antigen causing transgene expression by #episomal replication and maintenance of expression #plasmids carrying SV40 origin of replication. This is advantageous as it allows working both at early development stages (using TGE on CAP-T cells) and production levels (using SGE on CAP cells) without the need of changing the cell line. https://lnkd.in/gZ4mpgbZ
#CAP-T cells are emerging as an alternative to #CHO and #HEK cells for protein expression considering some inherent genetic aberrations in the latter ones, hindering expression of certain classes of proteins. Dive in for an introduction to these amazing cells! #CAP-T cells are a modification of #CEVEC's Aminocyte Production (CAP) cell line by transfecting them with E1 and pIX functions of #Adenovirus 5. They also constitutively expresses #SV40 large T antigen causing transgene expression by #episomal replication and maintenance of expression #plasmids carrying SV40 origin of replication. This is advantageous as it allows working both at early development stages (using TGE on CAP-T cells) and production levels (using SGE on CAP cells) without the need of changing the cell line. https://lnkd.in/gZ4mpgbZ
#Expi293 cells are a host of choice for many #biopharmaceutical companies and thus also a centerpiece of ongoing developmental #research. Lets look at an easy Expi293-F transient expression #protocol for #protein production in #Erlenmeyer flasks. #Expi293cells #thermofisherscientific #Biotechnology #mammaliancells #mammalianproteinexpression https://lnkd.in/duZcXynX
High Five™ Cells (BTI-TN-5B1-4) are a clonal isolate derived from the parental Trichoplusia ni cell line (cabbage looper ovary) and are commonly used for the expression of recombinant proteins using the #Baculovirus Expression Vector System (BEVS). #insectcellculture #baculovirusexpression #biotechindustry #biotechnology #protein #proteinexpression #bacmid https://lnkd.in/gMtFZYHr
#HEK293 cells are a host of choice for many biopharmaceutical companies and thus also a centerpiece of ongoing developmental research. Lets look at an easy HEK-F transient expression protocol for protein production in Erlenmeyer flasks. https://lnkd.in/gD8CdcKD #HEKcells #proteinproduction
#Insulators are a class of regulatory DNA elements that possess a common ability to protect genes from inappropriate signals coming from their surrounding environment. Incorporating #tDNAinsulators into your construct could help you achieve superior yield. https://lnkd.in/g9Hmicmp
In #biologics development, a lot of success depends on vector designing/ engineering as only an unique combination of the elements yields the best results. One of these vector elements are S/MARs (Scaffold/ Matrix Attachment Regions). Please give it a read here! https://lnkd.in/g4sxSVWV