The effects of the initial concentrations of Cr(VI) on chlorophyll-a (Chl-a), soluble protein and ultrastructure were investigated. Results showed that <0.5 and >1.0 mg L⁻¹ Cr(VI) stimulated and inhibited the growth of Isochrysis galbana, respectively. The tolerance mechanisms of I. galbana to Cr(VI) included the following: (1) increased activities of superoxide dismutase (SOD) and peroxidase (POX) for peroxidative damage resistance, (2) accumulation of Cr(VI) on the cell surface and inside the cell for detoxification and (3) conversion of intracellular Cr(VI) to less toxic Cr(III) as indicated by X-ray photoelectron spectroscopy (XPS) results. Cr(VI) enrichment by I. galbana may cause damage to marine ecology and human bodies through the food chain. The tolerance mechanisms of I. galbana to Cr(VI) may be potentially used to treat low-concentration Cr(VI) wastewater. Therefore, the responses and tolerance mechanisms of I. galbana to Cr(VI) must be further studied.