Processivity in early stages of transcription by T7 RNA polymerase
CT Martin, DK Muller, JE Coleman - Biochemistry, 1988 - ACS Publications
CT Martin, DK Muller, JE Coleman
Biochemistry, 1988•ACS PublicationsMaterials and Methods 77 RNA polymerase was prepared from E. coli strain BL21
containing plasmid pAR1219 (kindly supplied by William Studier and John Dunn), with T7
gene 1 (RNA polymerase) cloned under inducible control of the lac UV5 promoter
(Davanloo et al., 1984). Enzyme was purified as previously described (King et al., 1986) by
fractionation with Polymin P (less than 1.25%) and ammonium sulfate, followed by
containing plasmid pAR1219 (kindly supplied by William Studier and John Dunn), with T7
gene 1 (RNA polymerase) cloned under inducible control of the lac UV5 promoter
(Davanloo et al., 1984). Enzyme was purified as previously described (King et al., 1986) by
fractionation with Polymin P (less than 1.25%) and ammonium sulfate, followed by
Materials and Methods
77 RNA polymerase was prepared from E. coli strain BL21 containing plasmid pAR1219 (kindly supplied by William Studier and John Dunn), with T7 gene 1 (RNA polymerase) cloned under inducible control of the lac UV5 promoter (Davanloo et al., 1984). Enzyme was purified as previously described (King et al., 1986) by fractionation with Polymin P (less than 1.25%) and ammonium sulfate, followed by
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