Birmingham Tissue Analytics

#TissueTuesday: Examining a Sjögren's disease salivary gland biopsy with Akoya Biosciences, Inc.'s Phenocycler-Fusion 28-plex panel. Sjögren's is an autoimmune disorder where the body targets fluid-producing glands, causing dry mouth and eyes. This image captures the dynamic interaction within tertiary lymphoid structures, revealing CD3+ T cells (🟠) and CD20+ B cells (🔵), alongside key markers like FoxP3 (🟡), PD-1 (🟢), Granzyme B (🔴), and Interferon Gamma. These markers highlight the interplay between inflammatory and regulatory immune cell populations in this disease. #SjögrensDisease #Immunology #ImmuneResponse #Phenocyler

🧬 #TissueTuesday | Molecular Cartography of Bone in collaboration with Georgiana Neag 🦴🔬 This image showcases the power of ACD’s RNAscope technology, revealing Sp7 (🟢), Sost (🔴) and Dmp1 (🔵) mRNA transcripts in FFPE cortical bone of a mouse, with DAPI nuclear staining in grey. Captured with Akoya Biosciences, Inc.’s phenoimagerHT at 10x magnification, it highlights the precision of molecular mapping in tissue! #RNAscope #Histology #BoneBiology #PhenoImager

This week’s #TissueTuesday features a multiplex IHC staining of a synovial biopsy from a Rheumatoid Arthritis patient, captured using the Akoya Biosciences, Inc. Phenocycler! 🧬🔬 The image reveals the intricate cellular landscape, with fibroblasts and macrophages in the lining layer, while T cytotoxic, helper, and regulatory cells cluster around the blood vessels.  Stained as part of a 30-plex panel , this image provides valuable insights into the immune interactions driving inflammation. 🌟 #MultiplexIHC #AkoyaPhenoCycler #RheumatoidArthritis #ImmuneCells #Fibroblasts #Macrophages #TCells #CellularInsights 

📢 New Publication Alert! We are proud to share an exciting new paper, co-authored by members of Birmingham Tissue Analytics, investigating the spatial organisation of the gastric microbiota in chronic gastritis and gastric intestinal metaplasia — key precancerous stages in stomach cancer development. 🔬 This study highlights the presence and spatial distribution of Helicobacter pylori and non-H. pylori bacteria, shedding light on how microbial invasion of the gastric lamina propria may contribute to cancer progression. 👏 A special congratulations to our current team members Ana Teodósio and Jordanne Jones, and to former team members Jack McMurray and Kelly Hunter for their contributions to this important work. 🤝 It was a pleasure to work with Harriet Giddings, Amanda Rossiter and the rest of the team! We look forward to working together in the future. Read the full paper here: https://lnkd.in/ePszRZb3 #SpatialBiology #Microbiome #CancerResearch #GastricCancer

This week's #TissueTuesday highlights a vibrant hyperplex immunofluorescence image of tonsil tissue, serving as a positive control in a study on tertiary lymphoid structures (TLS) 🌟🔬 Featuring key markers designed for TLS research, this image was acquired on the Lunaphore (a Bio-Techne brand) COMET 1.0, enabling high-plex spatial analysis with exceptional precision. 🌐 DAPI 🔴 GranzymeB 🟡 Cytokeratin 🟢 FoxP3 🔵 VE-Cadherin 🟣Ki67 #Immunofluorescence #SpatialBiology #Tonsil #TertiaryLymphoidStructures

#TisssueTuesday 🧬 Cancer immunology is evolving at an incredible pace, and understanding the tumour microenvironment can help gain insights into anti-tumour immunity and discovery of biomarkers that may guide personalised immunotherapy. Working with Natalie To, we designed a multiplex immunofluorescence panel to look at immune cell aggregates in oesophageal cancer using PhenoImager HT by Akoya Biosciences, Inc.. This technology enhances our ability to explore the immune landscape with unprecedented precision! #CancerResearch #ImmunoOncology #MultiplexIF #OesophagealCancer #TumourMicroenvironment #Akoya #PhenoImager

Join our upcoming Visiopharm User Group Meeting in the UK - a day of learning, networking, and case study presentations from peers. Our expert speakers come from Birmingham Tissue Analytics, Propath UK, HistologiX, OracleBio, University of Leicester, and Charles River Laboratories. Date: Tuesday, 29 April 2025 Time: 9:00 AM – 4:30 PM Location: University of Birmingham Medical School, Birmingham   Secure your place now: https://lnkd.in/d3Zqyemm #Staycurious

This #TissueTuesday, we're taking a closer look at a salivary gland biopsy from a Sjogren's disease patient using the Bruker Spatial Biology GeoMx® Digital Spatial Profiler (DSP). Stained with four fluorescent markers - blue for the nucleus and three for key proteins - this image highlights four distinct regions of interest selected for targeted sequencing!

This week's #TissueTuesday features a stunning multiplex immunofluorescence image of tonsil tissue, showcasing key markers like Vimentin (endothelial/blood vessels), CK (epithelium), Foxp3 (T-regs), and ICOS (activated/antigen-experienced T-cells). Captured with the Lunaphore (a Bio-Techne brand) Comet 1.0, this advanced system allows for the staining of up to 40 markers per run, providing intricate insights into cellular interactions! 🔬 #Lunaphore #Immunofluorescence

Excited for this week’s #TissueTuesday! Today, we’re showcasing a multiplex immunofluorescent image of Tertiary Lymphoid Structures (TLS’s) found in the salivary glands. This image was captured using the Akoya Biosciences, Inc. Phenocycler Fusion 2.0, highlighting a range of immune cell populations. Stay tuned for more #TissueTuesday insights as we dive deeper into cutting-edge imaging techniques!