ION Biosciences

New product alert: BAPTA JF™549, a calcium indicator designed for imaging calcium transients in neurons and cardiomyocytes. With brightness similar to Oregon Green Bapta-1, and a red-shifted excitation and emission to reduce background. Try it today: https://lnkd.in/gMrtTcyS #Neuroscience #CalciumImaging #Cardiomyocytes #CalciumIndicator #NewProduct

Discover our newly launched Reference Library, now available on our website. Dive into exciting research using Fluo-4, a calcium indicator that’s led to countless discoveries in the past few decades. Discover key studies demonstrating the efficacy and various uses of Fluo-4 and see how it can enhance your research. Access a wide range of applications and expertise with just a few clicks in our Reference Library. Explore it here: https://lnkd.in/gSzmiEDq #CalciumIndicators #Fluo4 #CalciumImaging #CellResearch #ScientificDiscovery #FluorescentDyes #InnovativeResearch #LabTools

We are excited to announce the launch of our new Reference Library, now accessible on our website, featuring some of the most exciting studies from our research community. Here you can discover some of the applications of Fura-2, a ratiometric calcium indicator for accurate monitoring of intracellular calcium dynamics. Scroll to explore just a few examples of what’s possible with Fura-2, then use our Reference Library to discover more of what you can achieve with our dyes, in just a few clicks! Link to the Reference Library: https://lnkd.in/gSzmiEDq #CalciumImaging #FluorescentDyes #CellularAssays #ResearchInnovation #PublicationSpotlights #CellBiology #ScientificPublications #ProductSpotlights

We are thrilled to introduce BAPTA JF™549, our latest fluorescent calcium indicator designed for calcium imaging in neurons and cardiomyocytes. Key Benefits: Enhanced Performance: Excellent brightness and photostability for imaging fast calcium dynamics. Red-Shifted Fluorescence: Minimimal tissue autofluorescence for clearer imaging. Versatile Applications: Ideal for multicolor imaging and use with optogenetic tools. Read the seminal manuscript here: https://lnkd.in/ghHEER6h View more product info here: https://lnkd.in/g9g7P5FV

With the launch of our Reference Library resource - we’re taking a moment to showcase some of the Brilliant work published by our incredible users. Starting with the largest collection of fluorescent potassium indicators, our IPG (ION Potassium Green) series of dyes is unmatched when it comes to measuring changes in potassium concentrations for a wide array of applications. Scroll to explore just a few examples of what’s possible. Then use our Reference Library to discover more of what you can achieve with our dyes, in just a few clicks! Link to the Reference Library: https://lnkd.in/gSzmiEDq #potassiumIndicator #fluorescence #ionchannels #cellAssays #ProductHighlights #PublicationSpotlights

This study explored the critical role of potassium efflux in inflammasome activation induced by nsPEFs, offering insights into the mechanisms driving immune responses to cellular damage. IPG-2 facilitated accurate measurement of intracellular potassium dynamics, showing the role of potassium efflux in nsPEF-induced inflammasome activation. By monitoring IPG-2 fluorescence changes over time, the researchers discovered the immediate decrease in intracellular potassium levels following exposure to nsPEFs, revealing the molecular mechanisms driving immune responses to cellular damage. The use of IPG-2 allowed researchers to evolve the impact of nsPEFs on inflammasome activation, providing a deeper understanding of the dynamics of immune signaling. Read more about this incredible research here: https://lnkd.in/dv5Wa2Zt  #ResearchInsights #ImmuneSignaling #InflammasomeActivation #IPG2

Measure signal from the cells you care about, not the ones you don't - even in high throughput screening. With ION Thallos-HTL, you can localize a thallium indicator to cells expressing Halotag only - enabling you to get cell phenotype specific data in complex culture systems. Read more about how ION Thallos-HTL can take your screening to the next level, the cellular level: https://lnkd.in/gdzUEMkD #hts #drugdiscovery #cocultures #innovation #ionchannel #assays

Our latest innovation...a reference library! A resource created for our users, so you can find the most relevant publications for your research in just a few clicks. Filter by ION product, cell type, target protein, and/or application to find the right protocols and resources for you. Start your discovery today! - https://lnkd.in/gSzmiEDq #Publications #Science #Accessibility #KnowledgeDatabase

In a recent study, researchers explored the critical role of potassium (K+) efflux in NLRP3 inflammasome activation during immune cell attacks. To do so, they used the fluorescent K+ indicator IPG-2 AM (Ion Potassium Green-2 AM) a highly efficient tool for assessing intracellular K+ concentration by flow cytometry. The use of IPG-2 AM was decisive in delineating the mechanisms by which K+ efflux triggers inflammasome activation and subsequent immune responses. In order to monitor changes in K+ concentration during cytotoxic T lymphocyte (CTL) attacks, target cells were stained with IPG-2 AM and conjugated with CTL. The researchers noticed a rapid effector-to-target (E) efflux of intracellular K+, dependent on the effector-to-target ratio, in pulsed target cells. This rapid K+ efflux coincided with the kinetics of caspase-1 activation, strongly suggesting that K+ efflux after target cell puncture serves as an initiation signal for caspase-1 processing. Their use of IPG-2 AM in this study was essential to visualize and quantify intracellular K+ dynamics during immune responses. This focus allowed the researchers to discover a new perforin-activated pyroptosis pathway involving K+ efflux, NLRP3 inflammasome activation, caspase-1 activity, and GSDMD-dependent pyroptosis. These findings contribute significantly to the understanding of CTL-mediated knockdown and the complex mechanisms underlying immune responses. Explore IPG-2AM here: https://lnkd.in/gFhCj5T7 #ScientificResearch #Immunology #Inflammasome #IPG2 #PotassiumEfflux #IPGAM #Pyroptosis #CellBiology #CTL #ImmuneResponse

At ION Biosciences, democratization is a fundamental value. Science should be accessible to all. We offer competitive pricing without compromising on excellence, and innovative assays that enable you to conduct cutting-edge research on most fluorescence-based instruments. Empower your research with our democratized solutions. Discover more about how we are leveling the playing field for scientists everywhere. Learn more about our latest products!   https://lnkd.in/gQVVPBy7 #IONBiosciences hashtag#innovation hashtag#scienceandtechnology hashtag#biotechnology hashtag#drugdiscovery hashtag#pharmacology hashtag#scientists