🎯 Ming "Tommy" Tang’s Post

https://lnkd.in/gWSXzeDf 27 June https://lnkd.in/g-9SC5wM 26 June https://lnkd.in/gawCRagn 26 June https://lnkd.in/gY69Fhqg 19 June https://lnkd.in/gye_FiFz A technique that harnesses ‘jumping genes’ — mobile genetic sequences naturally found in bacteria that can cut, copy and paste themselves into genomes — could hold the key to redesigning DNA at will. Guided by an RNA molecule called a ‘bridge’ RNA or ‘seekRNA’, the system has been shown to edit genes in a bacterium and in test-tube reactions, but it is still unclear whether it can be adapted to work in human cells. If it can, it could be revolutionary, owing to its small size and its ability to make genetic changes that are thousands of bases long — much larger than is practical with the CRISPR — without breaking DNA.

Is p53 the guardian of the genome? Our body is made up of many cells. Like humans, cells can make errors with performing different tasks. Enter p53! a gene which can help regulate the cell’s DNA form becoming mutant. However, even with the help of p53, some cells still make errors and become cancerous. This article will talk about p53’s role and function and also it’s effectiveness when regulating the cell. The process starts with the DNA damage being detected. p53 is most commonly found in Arg Codon 72 where it is expressed by proteins. Here, p53 stops the cell it's current stages of the cell cycle of proliferation. This “freezes” the cell from continuing any activities. p53 then interacts with the cyclin B or cdc2 genes of the cell, these sections of the genome promote mitosis. With the cell “frozen”, p53 can freely repair the DNA. Once completed, p53 is finished, it restarts the cell cycle and allows the cell to proliferate. If it can't be fixed, p53 initiates Apoptosis, set of steps to kill the cell. This process is effective in regulating the cells health. Because p53 is a key player in this process, The overexpression of p53 makes its position in the genome more prone to methylation and becoming silenced. Due to this, even with the help of p53, the cell can still become benign and malignant. In conclusion, I think p53 is a useful tool in regulating cell health for short periods of time. But when cell and errors become too much, they can “adapt” adapt to p53 and silence it.

Why choose CeGat's ExomeXtra®? It combines the strengths of WGS, WES, and array CGH by overcoming their limitations. With enhanced sensitivity and coverage, it goes beyond exome coding regions to include the entire mitochondrial genome and known disease-causing regions. CeGat's innovative design and in-depth analysis make ExomeXtra® the most comprehensive genetic test available. As CeGaT's exclusive partner in the region, ImagineHealth is here to help you gain access to the latest groundbreaking healthcare solutions. Contact us for more information. #geneticdiagnostics #tumordiagnostics #ImagineHealth #CeGaT #Genetics #Diagnostics #Treatment #Healthcareindustry #innovation

Another very exciting work! DNA i-motifs or DNA knots [Dnots;) "hemi-protonated intercalated cytosine base pairs folded into a tetrameric structure"] are important regulatory elements spread across the genome (>50k, ~50% intergenic) and related with genes upregulated during early cell cycle phases. It will be fantastic to see results from DNA i-motifs in human primary cells and tissues, in healthy and various disease contexts as well as in regard to cell cycle control! 👌

Did you know? Whole genome sequencing can detect variants in the non-coding region of DNA, certain spinal muscular atrophy variants, repeat expansions, and mitochondrial variants, which may eliminate the need for additional testing. Learn more. https://bit.ly/44ecABk

To understand how Sasha's mutation can be fixed, you need to start with RNA splicing. Before a protein is created from DNA, the DNA is first 'transcribed' into RNA. Then an important step happens at the RNA level: non-coding regions called 'introns' are removed, and the coding regions called 'exons' are spliced together. Mutations can cause problems with the splicing process, leading to combinations that fail to produce functional protein. >15% of all disease-causing genetic mutations impact splicing! So fixing Sasha's mutation will pave the way to curing millions of people of all kinds of diseases in the future: https://lnkd.in/gsFaYycf #rnatherapeutics #SavingSashaFromSLC6A1

Alterable/Programmable RNA base editing by dint of targeted modifications. Clustered regularly interspaced short palindromic repeats (CRISPR)-based genome editors are revolutionary tools in biology and hold exalted pawns for the treatment of human diseases. Advanced DNA base editing tools, such as cytosine and adenine base editors, have been developed to correct permanent mistakes in genetic material. Regardless of this, undesired off-target edits would also be permanent, which poses a considerable risk for therapeutics. Alternatively, base editing at the RNA level is capable of correcting disease-causing mutations but does not lead to lasting genotoxic effects. RNA-based editors offer temporary and reversible therapies and have been catching on in recent years. Here, RNA editors based on A-to-inosine, C-to-U and U-to-pseudouridine changes. the programmable RNA-targeting systems as well as modification enzyme-based effector proteins and recent technological breaches.

Combination of genome-wide rare variant-based linkage analysis and ultra-long read genome sequencing revealed molecular diagnosis of Facioscapulohumeral Muscular Dystrophy Type 1 in a family https://bit.ly/3Tp6Xgb #GIMO

How to Find if Virus Genome RNA or DNA, Single or Double Stranded? The viral genome is packed inside a symmetric protein capsid, composed of either a single or multiple proteins, each of them is encoding a single viral gene. Due to this symmetric structure, viruses could encode all the necessary information for constructing a large capsid using a small set of genes. Youtube video: https://lnkd.in/dmq_jFE3 \#nikolays_genetics_lessons

https://lnkd.in/e8QGYv4V A bacterial reverse transcriptase that is using RNA to make new genes!! Science is beautiful