salmonella detection 25 g : 1️⃣ weight the sample 25 g and add primary Enrichment ( pre warming BPW at 37_40c°) 225ml *If the sample is chocolate 🍫 or cocoa powder the primary enrichment is (UHT Milk ) and we add 0.45 ml brilliant green solution for initial suspension 250ml and leave the sample at lab temperature for 20_30 min and homogenise by stomacher (#BAM_Method ) 2️⃣ Incubate the primary Enrichment at 37 c° for 18_20h 3️⃣ transfer step for selective Enrichment: - transfer 0.1ml into tube contain 10 ml RVS broth and incubate at 41.5c° for 24 h - Transfer 1 ml into tube contain 10 ml MKTTN and incubate at 37 c° for 24 h 4️⃣ after incubation Streaking on XLD and SS agar and incubate at 37 c° For 24 h *️⃣ Typical colonies of Salmonella are Red colonies with black center
Ahmed Ibrahime Abdelrazik’s Post
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Black sesame seeds can have different specifications depending on the supplier: Purity: Can range from 95/5 to 99.99% Moisture: Can be up to 5% FFA: Can be up to 2% Admixture: Can be a minimum of 0.01% Oil: Can be a minimum of 48% Total plate count: Can be up to 20,000 cfu/gm Yeast and mold: Can be up to 5,000 cfu/gm Staphylococcus aureus: Should be absent per gram E. coli: Should be absent per gram Salmonellae: Should be absent per gram Bhc/DDT/Aldrin: Should not be detected
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Gram staining was discovered by Christian gram in 1884 . Gram staining is used to distinguish gram positive and gram negative bacteria. Gram positive bacteria contain thick layer of peptidoglycan and do not contain pilli and porins. It's examples are- Streptococcus, clostridium, mycobacterium etc. Gram negative bacteria contain a thin layer of peptidoglycan which is sorrounded by lipopolysaccharide layer. Gram negative bacteria contain pilli and porins. it's examples are E coli , mycoplasma, salmonella etc .
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Test for absence of bile tolerant gram negative bacteria in product 🌹Prepare 1:10 sample dilution by add 1g or 1 ml to 10 ml of TSB, and Incubate at 20 - 25 C for 2-5 h 🌹Transfer 1ml from TSB into 100ml of mossel entrobacteriaceae enrichment broth ( MEEB) onto surface of violet red bile glucose (VRBG), Incubate at 30 - 35C for 18-24 hr 👉👉👉👉Observation 🎯Presence red colonies surrounded by reddish precipitate 🎯NO growth, product complies with test for absence of bile tolerant gram negative bacteria
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DETAILED SPECIFICATION YELLOW CORN (Animal Feed) Specification: 1) Protein: Min 8.00% 2) Moisture: Max14.00% 3) Fiber: Max.2.70% 4) Total Ash: Max 1.50% 5) Fat: Max. 3.50% 6) Total Aflatoxin: 20 ppb max 7) TVN: 50GM/100 GM max 8 Broken kernels: 5% max 9) Kernels damaged: 0.5% max 10) Foreign materials: 0.5% max 11) Toxic grains: 2-3/100 Gms max 12) Bulk density: 60 - 70 hecto-liters min 13) Total count: 5*10^5 14) E Coli: Absent in 01.1 GM 15) Salmonella: Negative / Absent in 25 Gms 16) Mould Colony: 5*10^5 Thank You +62 895 2811 8585
Laboratory Supervisor at Silo Foods For Food industreis
4moVery helpful! But where's the confirmation test To ensure that these colonies are salmonella