Alithea Genomics’ Post

Want to learn something new? We have prepared a blog article about High-Definition Single-Cell RNA Sequencing (HD scRNA-Seq) for you! 🧬 This cutting-edge technology enhances the resolution of single-cell transcriptomics, enabling researchers to dive deeper into cellular diversity and gene expression. But one of the biggest challenges remains: cell isolation. That's where our new benchtop instrument @Lexogen single cell dispenser comes into play, offering a unique workflow, from cell to HD RNA-Seq data, validated by Lexogen’s scientists even from suspensions as low as 100 cells! Rare cells are now within reach for groundbreaking research. ➡️Read more about HD scRNA-Seq here: https://lnkd.in/deSiY2Zv ➡️Find more information on Lexogen single cell dispenser here: https://lnkd.in/d2_SNqHD #scRNASeq #SingleCell #Transcriptomics #CellIsolation #RareCells #Lexogen

Synthetic antisense oligonucleotides can modulate RNA function, influencing gene expression levels, exon skipping, and epitranscriptomics. However, understanding the function of various RNAs and the proteins they interact with can take time and effort. Antisense technology promises to deliver therapeutics for treating diseases by targeting RNA. Bio-Synthesis offers a comprehensive suite of technologies to enable your RNA research and help answer these critical questions. Klaus D. Linse, Ph.D.

📍 Private information leakage from single-cell count matrices Walker et al. demonstrated that individuals in single-cell gene expression datasets are vulnerable to linking attacks, where attackers can infer their sensitive phenotypic information using publicly available tissue or cell-type-specific expression quantitative trait loci (eQTLs) information. Highlights 🔹 Single-cell RNA sequencing can reveal private information through linking attacks 🔹 Individuals can be reidentified using publicly available data with or without eQTLs 🔹 Linking accuracy is high even with minimal pre-processing of single-cell count matrices 🔹 Linking remains accurate even when eQTLs are derived from different cohorts. ➡️ More details: https://lnkd.in/euh3jS_C #spatialomics #spatialbiology #singlecellanalysis #singlecell #ColumbiaUniversity

Unlike traditional bulk RNA-Seq which averages gene epxression across entire cell populations, single cell RNA Seq (scRNA-Seq) provides a high-resolution view by capturing gene expression profiles from single cells. This enables research to unravel the heterogeneity within tissues, uncover rare cell types and decipher dynamic cellular processes. Marker genes play a pivotal role in scRNA-seq analysis in allowing us to annotate the various cell types and the selection of these marker genes (typically <20) are crucial. There are a variety of marker gene selection methods available. However, each method has strengths and limitations, making it difficult to choose the optimal marker gene identification method for a given dataset. In this recent paper by Pullin and McCarthy, they compared 59 computational methods for selecting marker genes in scRNA-seq data. Read it here: https://lnkd.in/dzCQYbyN If you are unsure about which method to use in your scRNA-Seq data analysis, we're more than happy to walk you through the best method to use for your data! Contact us today at info@insigene.com 📧 #bioinformatics #scRNASeq #transcriptomics #RNA #dataanalysis #genes

Want to learn something new? We have prepared a blog article about High-Definition Single-Cell RNA Sequencing (HD scRNA-Seq) for you! 🧬 This cutting-edge technology enhances the resolution of single-cell transcriptomics, enabling researchers to dive deeper into cellular diversity and gene expression. But one of the biggest challenges remains: cell isolation. That's where our new benchtop instrument Lexogen single cell dispenser comes into play, offering a unique workflow, from cell to HD RNA-Seq data, validated by Lexogen’s scientists even from suspensions as low as 100 cells! Rare cells are now within reach for groundbreaking research. ➡️Read more about HD scRNA-Seq here: https://lnkd.in/deSiY2Zv ➡️Find more information on Luthor single cell dispenser here: https://lnkd.in/d2_SNqHD

🚀 Understanding functional genomics is critical to advancing genetic research and therapeutic discovery. 🎯 Dharmacon™ reagents support researchers on this journey at the DNA, RNA, and protein levels with the broadest range of functional genomic tools including industry-leading gene modulation and gene editing solutions that help elucidate complex biological networks for functional genomic studies. 🔬 From single-gene experiments to whole-genome screens, Dharmacon™ Reagents have you covered with a complete range of genomic solutions, facilitating advancements in genetic research and therapeutic discovery. 🧬 Known for almost 30 years as premier providers of synthetic oligonucleotides, Dharmacon™ reagents - now a part of Revvity - were the first to offer algorithm-designed synthetic siRNA for RNAi, and the first to provide guide RNA for CRISPR-Cas9 gene editing applications. 👉 Contact our team at sales@thesciencesupport.com to discuss your needs. #GeneticResearch #TherapeuticDiscovery #FunctionalGenomics #GeneEditing #Dharmacon #CRISPR #RNAi #Innovation #SyntheticOligonucleotides

Wow. Just wow. https://lnkd.in/euKAteGS This new bridge recombination technology represents a revolutionary leap in gene editing capabilities. Unlike #CRISPR and its variants, which excel at small edits but struggle with large genetic changes, this technique allows for the precise insertion, deletion, or inversion of substantial #DNA segments. This breakthrough could dramatically accelerate the development of advanced cell therapies for cancer, enable more effective treatments for genetic disorders like ALS and Huntington's disease by removing problematic repetitive mutations, and facilitate the creation of complex genetic circuits in synthetic #biology. 🦠 Key advantage: Can add, remove, or flip large DNA stretches - a major limitation of CRISPR which can only make small cuts. 🩺 Potential applications: • Uploading genes into #cancer cell therapies • Collapsing repetitive mutations in ALS and Huntington's • Replacing broken genes in inherited conditions 💊 Impact on drug development: • New approach for gene insertion in cell therapies • Potential for editing entire genomic regions • Could surpass limitations of CRISPR and prime editing 🔮 Future prospects: • Third wave of RNA-guided systems after RNAi and CRISPR • Competing with startups like Prime Medicine and Tessera 🚨 Current status: Tested in bacteria, needs adaptation for human cells What do you think? Will this surpass CRISPR? #geneediting #biotech #medicalinnovation #pharmaceutical

Excited to share our latest application note on the use of Countess Automated Cell Counters with single-cell RNA sequencing (scRNA-Seq) and single-cell sequencing assay for transposase-accessible chromatin (scATAC-Seq) technologies. These technologies provide high-resolution data at the single-cell level, offering new insights into gene expression and regulation. Our application note provides a comprehensive guide on how to achieve accurate counts of viable single cells or nuclei and covers everything from instrument setup, single-cell preparation and counting, aggregation minimization, and optimal concentrations for counting. For those working with scRNA-Seq or scATAC-Seq, this guide is a must-read!

𝗪𝗶𝗹𝗹 𝗰𝗶𝗿𝗰𝘂𝗹𝗮𝗿 𝗥𝗡𝗔 𝗯𝗲 𝗣𝗼𝗶𝘀𝗲𝗱 𝘁𝗼 𝗦𝘂𝗿𝗽𝗮𝘀𝘀 𝗟𝗶𝗻𝗲𝗮𝗿 𝗺𝗥𝗡𝗔 𝗮𝘀 𝗧𝗵𝗲𝗿𝗮𝗽𝗲𝘂𝘁𝗶𝗰 𝗦𝘁𝗿𝗮𝘁𝗲𝗴𝗶𝗲𝘀? ✳ Lots of funding seems to be pumped into circular RNA where other viral gene therapies may not yield target expression. #GeneTherapy #FutureOfMedicine 🧬 Increased Durability: Circular RNA offers greater stability than linear mRNA, with extended protein expression, which is crucial for effective therapies. #RNAResearch #Biotech 💰 Cost Efficiency: Manufacturing circular RNAs is projected to be 80-90% cheaper than mRNA, making it a more viable option for widespread therapeutic use. #CostSavings #Innovation Some experts even believe that circular RNAs will replace mRNAs in certain contexts. Let see how it looks in a couple of years time! read more here: https://lnkd.in/e88cpEGy https://lnkd.in/eyuHkUaq https://lnkd.in/efJ5tUk8 Image credit: https://lnkd.in/ebMi35ZK

Plasmid DNA applications are driving innovation across several fields. Here’s a quick overview of where we’re seeing incredible progress: 1. **Gene Therapy**: Plasmid DNA offers a safer alternative to viral vectors for delivering therapeutic genes, making gene therapy more accessible and reducing risks. 2. **Vaccine Development**: With DNA vaccines, we're seeing advancements in combating infectious diseases and cancer, thanks to the ease and speed of production. 3. **CRISPR and Genome Editing**: Plasmid DNA is crucial for delivering CRISPR-Cas9 components, enabling precise genome modifications and the potential to revolutionize treatments for genetic disorders. 4. **Synthetic Biology**: The field is leveraging plasmid DNA to engineer new biological systems, driving innovations in biofuels, pharmaceuticals, and more. The future of biotechnology looks brighter with these cutting-edge applications! #Biotechnology #GeneTherapy #CRISPR #VaccineDevelopment #SyntheticBiology #Innovation #Healthcare