Amar Nath Kar, Ph.D.’s Post

Cytosolic DNA sensing pathway (cGAS-STING)-based therapeutics The cGAS-STING pathway, a key player in innate antiviral immunity, senses cytosolic double-stranded DNA (dsDNA) regardless of origin, including mitochondrial (mtDNA). This triggers STING activation and subsequent nuclear translocation, leading to the induction of IFN-I genes critical for antiviral responses. This understanding sheds light on the role of cGAS in non-infectious inflammation, DNA damage response (DDR), aging, longevity, and neurodegeneration in rare diseases, such as lysosomal storage disorders, and common ones, like Parkinson's and cancer. Notable, some cGAS-STING regulating molecules are being evaluated in clinical trials. The article was published in Immunity: https://lnkd.in/eaXvqNtU #genetics #genomics #precisionmedicine #genomicmedicine #brain #neurology #neurodegeneration #neuroscience #parkinson #aging #longevity #raredisease #lysosomalstoragedisease #mitochondrialdisease #als #cancer #virus #antiviral #gaucher #taysachs #niemannpick #lipids #metabolism #clinicalresearch #clinicaltrials #physiology #therapeutics #biomarkers #biotechnology #innovation #research #science #sciencecommunication

You’ll see CD9, or Cluster of Differentiation 9, in a lot of processes, like cell signaling, adhesion, migration, and even immune responses. It’s also an excellent exosome marker 👓 This Microscopy Monday we’re looking at the Anti-CD9 (extracellular) Antibody that lets you easily look at CD9 expression whatever your research focus is. And because it only targets the extracellular part of CD9, it’s excellent for flow cytometry and live cell work. As well as being a known marker of exosomes (aka extracellular vesicles), CD9 plays a role in T cell activation, B cell regulation, and allergic mediator release from mast cells. You’ll also find it in the central nervous system, where it’s important for neurite outgrowth, cell migration, and making sure neurons have a working myelin sheath. So it’s an interesting protein to look at! On the left below, you can see CD9 expression in perfusion-fixed frozen mouse hippocampus sections. It’s stained with our Anti-CD9 (extracellular) Antibody (ANR-209) and visible in green thanks to goat anti-rabbit-AlexaFluor-488. You can CD9 in the pyramidal layer (P). On the right, we’ve pre-incubated the antibody with CD9 (extracellular) Blocking Peptide (BLP-NR209), which suppresses staining and serves as an excellent control. Cell nuclei are stained blue with DAPI. Take a look at the rest of the data here: https://lnkd.in/e9H2Ecwn #exosomes #extracellularVesicles #neuroscience #immunohistochemistry #antibodies

Plasma p-tau212 antemortem diagnostic performance and prediction of autopsy verification of Alzheimer's disease neuropathology A significant advancement has been achieved in Alzheimer's Disease (AD) diagnostics through a novel blood test. Researchers have developed an innovative immunoassay specifically targeting p-tau212, a key biomarker associated with AD, distinct from similar biomarkers like p-tau217. Highlights of the Discovery: 🩸 The new blood-based assay for p-tau212 demonstrates exceptional accuracy in AD diagnosis, rivaling the performance of the well-established p-tau217 assays. 📊 Evaluated across five cohorts encompassing 388 participants, p-tau212 proved effective not only in diagnosing AD but also in detecting amyloid and tau pathologies, critical indicators of the disease. 🧪 The assay's diagnostic accuracy and the magnitude of change in p-tau212 levels surpassed those observed for other markers such as p-tau181 and p-tau231. 🧫 Detailed examinations showed significant p-tau212 presence in the neurofibrillary tangles within the brain, a key feature of AD, underscoring its role in AD pathophysiology. From Nature Communications: https://lnkd.in/gfkKFNih #HealthTech #Diagnostics #Neuroscience

Gain new insights into Alzheimer's disease research with our application note. Explore the use of Thermo Scientific Volumescope SEM and Amira Software to visualize changes in neuron morphology resulting from MCI inhibition. Learn how Amira Software streamlines data processing and enables efficient analysis. Read the full application note here. #AlzheimersResearch #Neuroscience

Gain new insights into Alzheimer's disease research with our application note. Explore the use of Thermo Scientific Volumescope SEM and Amira Software to visualize changes in neuron morphology resulting from MCI inhibition. Learn how Amira Software streamlines data processing and enables efficient analysis. Read the full application note here. #AlzheimersResearch #Neuroscience

Gain new insights into Alzheimer's disease research with our application note. Explore the use of Thermo Scientific Volumescope SEM and Amira Software to visualize changes in neuron morphology resulting from MCI inhibition. Learn how Amira Software streamlines data processing and enables efficient analysis. Read the full application note here. #AlzheimersResearch #Neuroscience

Gain new insights into Alzheimer's disease research with our application note. Explore the use of Thermo Scientific Volumescope SEM and Amira Software to visualize changes in neuron morphology resulting from MCI inhibition. Learn how Amira Software streamlines data processing and enables efficient analysis. Read the full application note here. #AlzheimersResearch #Neuroscience

Parkinson’s disease is characterized by vitamin B6-dependent inflammatory kynurenine pathway dysfunction Parkinson's disease (PD) is associated with dysregulated metabolism through the kynurenine pathway (KP). A multicenter study analyzed KP metabolites in blood and cerebral spinal fluid of PD patients and healthy controls. Results revealed increased neuroexcitatory quinolinic acid/kynurenic acid ratio in PD patients, linked to peripheral and cerebral inflammation, vitamin B6 deficiency, and PD clinical severity. KP metabolites accurately classified PD participants, and distinct KP profiles were associated with specific PD clinical features. These findings suggest that KP plays a crucial role in PD, integrating B-vitamin status, inflammation, and metabolism to influence clinical manifestation and probably therapeutics. The article is available as a Preprint: https://lnkd.in/e4QDaDyi. #genetics #genomics #precisionmedicine #genomicmedicine #brain #neurology #neurodegeneration #neuroscience #parkinson #metabolism #inflammation #immunity #neuroinflammation #biomarkers #therapeutics #biotechnology #innovation #research #clinicalresearch #science #sciencecommunication

Check out our new study: "Single-Cell Multi-Omics Map of Cell Type-Specific Mechanistic Drivers of Multiple Sclerosis Lesions." We identified a unique genetic signature in oligodendrocytes and spatially distinct metabolic changes in astrocytes at chronic active lesions in patients with progressive multiple sclerosis. Read more here: https://lnkd.in/eXfF2vXp #MultipleSclerosis #Neuroscience #SingleCellResearch

Small-molecule-induced epigenetic reprograming of lipid metabolism inducing CNS myelin regeneration Published in Cell, this article describes that oligodendrocytes in MS lesions are present but epigenetically silenced, preventing myelin production. Researchers developed a molecule (ESI1) that reverses this silencing, promoting remyelination in animal models and improving cognitive function in aged mice. ESI1 reprograms lipid metabolism in oligodendrocytes to enhance myelin production and potentially offers a new approach to treating demyelinating diseases. https://lnkd.in/edKwZa_C #genetics #genomics #precisionmedicine #genomicmedicine #epigenetics #omics #smallmolecule #brain #neurology #neurodegeneration #neuroscience #ms #multiplesclerosis #biotechnology #therapeutics #aging #longevity #dementia #innovation #research #science #lipids #metabolism #sciencecommunication