Aragen Life Sciences’ Post

One of the most basic concepts we learn in Biology 101 is DNA transcription and how promoters and enhancers guide this process (sort of). Understanding these elements (which we’re still figuring out) allows us to accomplish tasks we've been struggling with for decades, such as optimizing protein production for therapeutic purposes. Advances in synthetic biology have made this possible by enabling precise genetic and metabolic engineering. A prime example is the recent breakthrough in decoupling growth and protein production in CHO cells—a goal that seemed like a dream a decade ago. This study showcases how manipulating promoters and enhancers can lead to significant improvements in bio-pharmaceutical production. https://lnkd.in/dMJa-qM6 #Promoters #Enhancers #SyntheticBiology #Biopharmaceuticals

For early January, here’s an interesting paper where the yield and activity of a difficult to express enzyme is improved, in CHO cells, using a systems biology and metabolic engineering approach. As the authors suggest, the type of workflow they describe could be used to improve production of other biotherapeutics. #biopharmaceuticals #biotherapeutics #biotechnology #systemsbiology #omics #celllinedevelopment https://lnkd.in/d_2Qp7ek

Preparation Techniques of Polypeptide - Genetic Engineering Recombination. The technology for producing #peptides by this method has become mature and has the advantages of not being limited by production raw materials and strong expression directionality. Gene recombination methods are often used to synthesize peptides with relatively long peptide chains, especially peptides composed of hundreds of amino acids. The #genetic recombination method can recombine the gene sequence that regulates polypeptide synthesis into the genetic material of microorganisms, etc., and translate and express the genetic material in the recipient organism. The yield and purity of the polypeptides obtained by this method are greatly improved, and it is currently one of the effective ways to obtain certain specific biologically active peptides in large quantities. However, it is limited to the synthesis of macromolecular peptides, and it is difficult to express short peptide chains. In addition, in recent years, consumers in Europe and the United States have generally opposed foods and health foods that have undergone #genetic #bioengineering. #peptide #enzyme #geneticengineering #biological #biology #biopharmaceuticals #pharmaceutical https://lnkd.in/gGmsgEUD

Future Prospects of Recombinant Enzyme Technology. As technology advances, the potential of recombinant #enzymes continues to expand. Researchers are continuously uncovering new enzymes with unique properties, enabling more precise genome editing techniques like CRISPR-Cas9. The widespread adoption of CRISPR technology has revolutionized #geneticengineering, allowing scientists to edit DNA sequences with unprecedented ease and accuracy. Moreover, efforts are being made to engineer recombinant enzymes with enhanced catalytic efficiency. stability. and tolerance to extreme conditions, opening doors to new possibilities in biotechnology and medicine. #biology #bioengineering #biopharma #biopharmaceuticals #biomedical https://lnkd.in/gGmsgEUD

【Biology/Environment】 Enhancing Recombinant Protein Expression in Lettuce Recombinant proteins are crucial in pharmaceutical and other industries. A production method uses plants as hosts in transient protein expression systems. Researchers at the University of Tsukuba developed a technique to increase recombinant protein expression in lettuce by inhibiting RNA-dependent RNA polymerase. This technique achieved the highest protein expression levels reported in lettuce to date and is a promising approach for large-scale protein production. Read more details here; https://lnkd.in/eJyXzwv8 Original Paper; https://lnkd.in/e5-jeC7c

What is the future of microbial expression systems? 🦠 Microbial expression systems are widely used to manufacture diagnostic and therapeutic proteins. However, the commercialization success rate for new drug developments is less than 3%, partly due to high development and manufacturing costs, long turnaround times, and challenging product safety profiles. 💸 CDMOs, including KBI, are jumping in to update microbial expression systems to surpass the modest yields of traditional E. coli models. KBI Biopharma has developed PUREcoli, a microbial expression system incorporating over 1,000 gene edits to enhance protein production. 📈 Learn more about it in our latest article! ⬇️ https://lnkd.in/d-KT-xbg #biotechinnovation #proteinproduction #therapeuticproteins #CDMOs #drugdevelopment #biomanufacturing #biotechresearch #biotechindustry

Exciting insights from a recent study on #TargetedProteinDegradation . This research leverages a CRISPR-based transcriptional activation screen to uncover E3 ligases that facilitate heterobifunctional compound-mediated target degradation. The discovery of FBXO22 as a critical E3 ligase demonstrates its potential in supporting ligand-induced protein degradation. This advancement broadens the scope of TPD applications, especially in degrading challenging targets like FKBP12 and BRD4. By expanding the repertoire of E3 ligases, we can enhance the effectiveness and versatility of #PROTACs in drug discovery. #ChemicalBiology #Biotech #DrugDiscovery #ProteinDegradation #CRISPR #CancerResearch

Insights gained from omics studies on GS-CHO cell lines have great potential to inform the development of next-generation expression platforms. For example, genome and epigenetic maps can inform precise cell engineering work. We may also potentially identify improvements to the expression vectors as a result of gaining deeper understanding of the DNA sequences that work best within the wider CHO transcriptional landscape. In this article published in the latest September 2024 issue of Bioprocess International, my colleague Alessandro Di Cara and I review some of the developments in CHO omics knowledge, and consider where we may best apply such information for creating improved bioprocessing outcomes. https://lnkd.in/eBPGJPTi #LonzaExpressionSystems #LonzaInYourLab #Biologics #Biotechnology

The path to Precision Biomanufacturing! this article describes our current work, using omics clone characterization to design the next generation of CHO cells.

📢 Boost Protein Production: Game-Changing Method Revealed! 🧬 Researchers enhanced protein production in Trichoderma reesei by integrating a plasmid with the eGFP gene. 🔍 The study found that using the tef1 promoter enables multiple gene copies to integrate, boosting protein output. 💡 Findings suggest using this method could significantly benefit biopharmaceutical production and other industries. 📊 The approach may lead to more efficient industrial-scale protein synthesis. #BioprocessUpdates #Bioproduction #ProteinSynthesis #TrichodermaReesei #eGFP #Tef1Promoter #GeneticEngineering #Biopharmaceuticals #IndustrialBiotechnology #SyntheticBiology #ProteinProduction #MulticopyIntegration #ResearchInnovation #Bioengineering #SustainableBioproduction #ScienceForGood ▷ Read the full article here: 📎 https://lnkd.in/d_tXvQHT