Ideal for those of you in the oil, cosmetics, hygiene, and cleansing industries, the DrySyn Vortex Blend is an overhead stirrer system, that allows you to test new formulations and blends in beakers rather than round-bottomed flasks (this insert is available too). If multiple experiments are required in parallel in your R&D lab, there can be problems related to fume hood space as well as consistency in stirrer motor speed and power. The Vortex Blend presents a solution: a space-saving three-way gearbox and heating plate, ensuring identical conditions in terms of stirring rate and temperature, allowing you to stir your mixtures simultaneously with one motor. #overheadstirrer #parallelsynthesis #cosmetics
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#EXCIPIENTS FOR #ROOM #TEMPERATURE #STABLE #FREEZE-#DRIED #MONOCLONAL #ANTIBODY #FORMULATIONS In contrast to #monoclonal #antibody formulations containing only #sucrose as a #lyoprotectant, which requires longer #primary #drying times at low temperatures to avoid collapse, besides the refrigerated storage, Haeuser et al. (2020) proposes a shorter and more #aggressive #freeze-#drying cycle for such formulations, enabling storage at room temperature through the use of suitable excipients and an #optimized #lyophilization #cycle. Combinations of hydroxypropyl-beta-cyclodextrin (#CD), #recombinant #human #albumin, #polyvinylpyrrolidone (#PVP), #dextran 40 kDa (#Dex), and #sucrose (#Suc) were studied using two #mAbs at 10 mg/ml. Formulations containing CD/Suc at 24 and 56 mg/ml respectively, or CD/PVP/Suc at 24, 39.2 and 16.8 mg/ml respectively, with #glass #transition #temperatures ranging between -16oC and - 19oC approximately, in addition to #collapse #temperatures in the same range, namely -17.8oC for CD/Suc and -18.7oC for CD/PVP/Suc, showed superiority with respect to #stability #during #storage at 40oC when compared to pure #sucrose formulations. #Visible #particles, #turbidity, #color and #monomer #content were evaluated in this study. After an #aggressive #lyophilization #cycle, that is, with #primary #drying conducted at +10oC and #secondary #drying at +25oC, such formulations were characterized by pharmaceutically #elegant #cakes, #shorter #reconstitution #times and higher #Tg', #Tc, and #Tg. #CD/Suc and #CD/PVP/Suc formulations presented #Tg values (#glass #transition temperature of the freeze-dried product) of 153oC and 150oC, evidencing their high #stability, and suggesting #sufficient #immobilization of the #mAb at storage temperatures of 25oC and 40oC. Additionally, the authors suggested that the #low #residual #water content after storage and the low tendency to #crystallize of formulations containing #CD further the possibility for #storage at #room #temperature. It is important to mention that despite of both storage temperatures tested, that is, 25oC and 40oC, being well below the #Tg of CD-based formulations, a different #stability behavior was observed. The authors suggested that this is due to #local #motions or Beta-#relaxations associated with #CD, causing #protein #instability during storage at 40oC, which led to the hypothesis of #sucrose superiority in relation to #inhibition of #local #motions. Finally, it was suggested that #CD is a very promising alternative when used as an #excipient for #lyophilization of #mAb formulations, since in addition to increasing the glass transition temperature of the formulation, it also functions as an #amorphous #stabilizer, in contrast to #bulking #agents such as #mannitol, that tend to #crystallize during the freeze-drying process. Ref: C. Haeuser, et al. Excipients for room temperature stable freeze-dried monoclonal antibody formulations, J. Pharm. Sci. 109 (1) (2020) 807–817.
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7moThanks for sharing! The DrySyn Vortex Blend is great for those more viscous solutions that are a bit much for a round bottom flask!