Axon Patch-Clamp | Molecular Devices’ Post

⚡ Are you fascinated by the intricate workings of cells and the mysteries of neural activity? If so, we have an exciting opportunity for you to dive into the field of #electrophysiology and unleash your scientific curiosity!   On the page below, you'll find a treasure trove of information about electrophysiology and the #patchclamp technique. Gain a deeper understanding of how current and voltage changes across cell membranes drive cellular functions and contribute to neurological diseases. Learn about the essential components of an electrophysiology lab setup, including environment, optics, mechanics, and electronics. Understand how our #axon instrument portfolio, featuring amplifiers, digitizers, software, and accessories, provides a comprehensive solution for patch-clamping experiments of all scales.   We have curated a collection of resources, including webinars, customer stories, and application notes, to further enhance your knowledge and expertise in the field of electrophysiology. Click below to learn more! #neuroscience #actionpotential #neurodegenerative

Meet one of the teams behind our #TeamScience - the electrophysiology group! The Electrophysiology (ephys) group is combining cutting-edge tools for recording electrical activity, such as Neuropixels probes, with reagents for identifying and perturbing specific neuronal cell types in living brains. Their current focus is on improving the reliability and throughput of extracellular electrophysiology experiments. https://lnkd.in/gy9xz7n8

Smooth, vibration-free movement for electrophysiology... No matter if you are patch clamping or using Neuropixel probes, few things are as important in neurophysiology as smooth motion of electrodes. The stepper motor based micromanipulators previously used in patch clamping rely on rotating spindles to generate a linear motion. This results in nearly unavoidable lateral vibrations which can be transferred to the surrounding area (tissue or external solution). In contrast, our piezo driven manipulators directly generate linear motion - free of any vibration. Don't just take our word for it, see for your self. Either in the video below or in a demo. Drop us a message if you would like to experience the smoothest micromanipulators in your own lab. #vibration #patchclamp #ephys #neuropixels

Want to make the cardiac cycle (and associated elements) intuitive? Today we derived the Wiggers diagram during our ECG lab. This ugly figure is typically saved until the end to summarize everything. That is a wasted opportunity! Derive literally everything from the atrial and ventricular syncytial action potentials...in the process getting your students to think about (and understand!) the connections between electrical and mechanical events. #haps #aps #physiology #pedagogy

Fluidics is the application of flowing substances, such as liquids and gases, to system control and logic operations. Fluidics is analogous to electronics but uses flow and pressure instead of electrical charge. how's it playing out in respiratory physiology? I'll let Jean Léonard Marie Poiseuille and Gotthilf Heinrich Ludwig Hagen break it down. reference in comments. #respiratorycare #mechanicalventilation #mechanics #physiology

Electrophysiological recording with glass electrodes is one of the best techniques to measure #membranepotential dynamics and ionic currents of voltage-gated channels in neurons. However, artifactual variability of the biophysical state variables that determine recording quality can be caused by insufficient affinity between the electrode and cell membrane during the recording. In this study, Angelica Jameson et al. utilized membrane-coated glass electrodes for the purpose of reducing artifactual variabilities delivered by technical difficulties of Drosophila #electrophysiology. The results indicate that membrane-coated glass electrodes reduce the variability of biophysical parameters and eliminate artifactual noise components. This innovation holds the potential for reliable recordings in challenging model systems such as Drosophila central #neurons and represents a step towards optimizing intracellular electrophysiology in such contexts. Read the article: https://bit.ly/4bl9kHg Learn more about the the patch-clamp systems used for this experiment: https://bit.ly/3QNCvuo

An #electrophysiology rig designed by electrophysiologists, for electrophysiologists. The PatchScope Pro 3000 is a hands-free inverted patch clamp rig for pharmacological and network studies. Discover more https://buff.ly/3XoWQas #patchclamp

Incredible Scientific and Technological Applications of Sound (Part 4) Acoustic scalpels Doctors already use sound waves for medical procedures like ultrasounds and breaking up kidney stones, but scientists at Michigan State University have created an acoustic scalpel that is precise enough to separate even a single cell. Modern ultrasonic technologies make it possible to create a beam with a focus of several millimeters, but the new instrument has an accuracy of 75 by 400 micrometers. The general technology has been known since the late 1800s, but the new scalpel is made possible by using a lens wrapped in carbon nanotubes and a material called polydimethylsiloxane, which converts light into high-pressure sound waves. When properly focused, sound waves create shock waves and microbubbles that exert pressure on a microscopic level. The technology was tested by removing a single ovarian cancer cell and drilling a 150-micrometer hole into an artificial kidney stone. The authors of the technology believe that it could finally be used to deliver drugs or remove small cancerous tumors or plaques. It can even be used for painless operations, since such an ultrasound beam can avoid nerve cells. To be continued.. If you've read the article this far please like and subscribe - it really helps the channel. Open the link to find thousands of interesting articles: https://lnkd.in/dYdAdmgE #nikolaysgeneticslessons

𝗘𝘅𝗽𝗹𝗼𝗿𝗶𝗻𝗴 𝘀𝗶𝗹𝗲𝗻𝘁 𝘁𝗿𝗮𝗻𝘀𝗹𝗼𝗰𝗮𝘁𝗶𝗼𝗻 𝗶𝗻 𝗻𝗮𝗻𝗼𝗽𝗼𝗿𝗲 𝗿𝗲𝘀𝗲𝗮𝗿𝗰𝗵 Traditional nanopore studies often rely on detecting ionic currents to understand molecular passage. However, not all molecules leave an electrical trace, some pass through undetected, a phenomenon known as silent translocation. In a recent study, Daniel Burden et al. from Wheaton College investigated this process, focusing on the silent translocation of a Cyanine 5 derivative (sCy5a) through α-hemolysin (αHL) nanopores. By combining single-molecule fluorescence with single-channel electrical recordings, they confirmed that silent translocations do occur, even though they are infrequent. Their cutting-edge experimental setup used the Orbit mini bilayer recording device and MECA 4 OPTO-INV Fluorescence Microscopy Kit on an inverted microscope, allowing for simultaneous optical and electrical measurements in artificial lipid bilayers. This research challenges the assumption that molecular translocation always produces an electrical signal, suggesting that silent translocations could be more common than previously believed. Want to learn more about this topic? 🔗 Read the full article: https://ow.ly/i0z950Tg9C7 🔗 Learn more about simultaneous optical and electrical recordings: https://ow.ly/hJb150Tg9C5 🔗 Visit the Burden Lab: https://ow.ly/CcaQ50Tg9C6 #NanoporeResearch #LipidBilayers #Electrophysiology #SilentTranslocation #MECA #FluorescenceMicroscopy #MembraneBiophysics #Orbitmini

Cardiac Modelling in LS-DYNA requires Multiphysics coupling electrophysiology, mechanical and fluid dynamics. Using Ansys LS-DYNA, electrophysiology (EP) models enable the automatic generation of the Purkinje network which can be coupled to mono/bi domain models. With the creation and connection of Purkinje nodes and Myocardial nodes, alongside pseudo-ECG computation – this helps in the prediction of drug effects on ECGs.