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🚀 Thrilled to share our latest research, now published in Separation and Purification Technology! Our paper, "Scar-free tag removal by CASPON® enzyme with broad physicochemical stability in biomanufacturing – A case study of five proteins", showcases a breakthrough in recombinant protein production. A huge thank you to all co-authors for their incredible contributions to this work: Daniel Elsner, Christoph Köppl, Maximilian Kogler, Patrick Scheidl, Andreas Fischer, Christina Kröß, Bernhard Sprenger, Jakob Liu, Leo Jakob, Chris Oostenbrink, Rainer Schneider, Gerald Striedner, Alois Jungbauer, Cécile Brocard and Monika Cserjan! Key highlights: 🔬 CASPON® enzyme enables seamless, scar-free tag removal. ⚙️ Broad stability across temperatures, pH, and bioprocess chemicals. 💡 Streamlined downstream processing. 📈 High yields, purity, and soluble titers in a case study of 5 biopharmaceuticals. This platform process holds immense potential for simplifying and scaling biomanufacturing. Check out the full study here: https://lnkd.in/dDYDmefZ #Biomanufacturing #ProteinEngineering #BiotechResearch #CASPON

Protein Expression Market Worth $5.4 Billion Request Free Sample Report: https://lnkd.in/drSbJzyx Protein Expression Market by Type (E.Coli, Mammalian, CHO, HEK 293, Insect, Pichia, Cell-free), Products (Reagents, Vectors, Competent Cells, Instruments, Service), Application (Therapeutic, Industrial), End User, and Region - Global Forecast to 2027 𝐓𝐡𝐞 𝐜𝐨𝐦𝐩𝐚𝐧𝐢𝐞𝐬 𝐟𝐞𝐚𝐭𝐮𝐫𝐞𝐝 𝐢𝐧 𝐭𝐡𝐢𝐬 𝐫𝐞𝐩𝐨𝐫𝐭 𝐢𝐧𝐜𝐥𝐮𝐝𝐞: Thermo Fisher Scientific, Merck KGaA (Germany), GenScript Biotech Corporation (US), Agilent Technologies, Danaher Corporation, Sartorius AG (Germany), Takara Bio Inc., Bio-Rad Laboratories Inc. (US), Lonza Group AG (Switzerland), Promega Corporation , New England Biolabs, and Oxford Expression Technologies Other Companies that are Operating in this Market Globally: Sino Biological, Inc. Strand Therapeutics Ajinomoto Bio-Pharma Services Takara Bio USA, Inc. ProteinSimple, a Bio-Techne brand ABclonal Technology BPS Bioscience Inc. 3P Biopharmaceuticals Biognosys Takara Bio Europe Biovian JOINN Biologics US Inc.  Xbrane Biopharma AB 3PBIOVIAN #ProteinExpression #MolecularBiology #Biotechnology #RecombinantProteins #ProteinResearch 📌𝐒𝐨𝐮𝐫𝐜𝐞: promega.in 📌𝐃𝐢𝐬𝐜𝐥𝐚𝐢𝐦𝐞𝐫: I don't intend any copyright (DM me for credit & removal)

By moving away from restrictive biological approaches and instead using enzyme biochemistry, we are making DNA simple – we have eliminated the need for bacterial sequences, including antibiotic resistance genes. Learn more: https://lnkd.in/e8meAqY #biotechnology #pharma #biotech #genetherapy #dbDNA

🧬 Understanding Bioreactor Modes for Better Cell Growth 🧬 Choosing the right bioreactor mode can make a big difference in your cell culture success. The image below shows four common modes: Batch, Repeated Batch, Fed-Batch, and Perfusion. A. Batch Mode: How it works: Start the process, and let it run without adding anything new. Best for: Simple, short-term production of things like enzymes, antibiotics, or yeast cultures. Graphs: No media flow after starting; volume stays constant. B. Repeated Batch Mode: How it works: Similar to batch, but with periodic media exchanges to refresh nutrients. Best for: Extending the life of microbial cultures, such as E. coli or yeast, used in producing proteins or other biomolecules, while keeping the process relatively simple. Graphs: Spikes in media flow during exchanges; volume changes with each exchange. C. Fed-Batch Mode: How it works: Gradually add fresh media over time without removing the culture. Best for: Higher yields of products like monoclonal antibodies or recombinant proteins, especially with mammalian cells like CHO (Chinese Hamster Ovary) cells. Graphs: Increasing flow rate and volume over time. D. Perfusion Mode: How it works: Constantly add fresh media and remove waste at the same time. Best for: Long-term, high-density cultures for producing high-value biologics, such as therapeutic proteins, using mammalian cells. Graphs: Steady flow in and out; volume stays stable. What do the graphs mean? F-t (Flow rate vs. time): Shows how fast media is added or removed over time. V-t (Volume vs. time): Tracks how the volume in the bioreactor changes. Understanding these modes and their graphs can help you choose the best approach for your bioprocessing needs. #Biotechnology #Bioreactors #CellCulture #Pharmaceuticals #Bioprocessing 🔗 Paper Source: 10.1016/j.procbio.2016.09.032

Scaling up bioproduction is a complex process with several key challenges, ranging from selecting the right system to ensuring a seamless transition to cGMP facilities. Each step is critical to achieving efficient and cost-effective production while maintaining quality and compliance. What do you think is the biggest challenge in the process? 1️⃣ Choosing the optimal system (E. coli, Yeast, CHO cells) 2️⃣ Achieving efficient/high-level protein expression 3️⃣ Scaling up for cost-effective production 4️⃣ Transferring processes to a cGMP facility We’d love to hear your thoughts—share them in the comments below! #Bioproduction #GeneEditing #Biotech #InfinixBio #CellBiology

📣 Don't miss out on the pre-conference workshops for the 4th Applied Biocatalysis & Enzyme Engineering Summit 📣 https://ter.li/bzfuvv Build on your knowledge with these hands-on dedicated sessions before the main conference kicks off, delivered by industry leaders. Choose from: ✨ Workshop A: Delving Into Realistic & Transformative Enzyme Design Techniques to Revolutionize Biocatalysis-Informed Small & Large Molecule Development ✨ Workshop B: Optimizing Process Efficiency for Hybrid Molecules to Enhance Biocatalysis Success Outcomes Download the brochure to learn more: https://ter.li/bzfuvv #AppliedBiocatalysis #EnzymeEngineering #SmallMolecule #Biologistics

Explore #Microbiology_Culture Market: Growth, Demand and New Opportunities Report by Value Market Research. >> https://lnkd.in/d_izvSYT #Microbiology culture cultivates #microorganisms such as bacteria, fungi, and viruses in a controlled #laboratory environment. This involves providing the necessary #nutrients, temperature, and other #conditions to support the growth and #reproduction of microorganisms for #research, diagnostic, or industrial purposes. Thermo Fisher Scientific Becton Dickenson Merck KGaA Pharmaceutical Bio-Rad Laboratories bioMérieux HiMedia Laboratories GmbH Neogen Corporation Condalab Hardy Diagnostics Eiken Chemical #healthcareindustry #pharmaceuticalindustry #healthcareresearch #biotechnology #microbiology #research #diagnostic

Excited for publishing 2nd Analytical Chemistry paper on developing high resolution protein footprinting. Our development is enhancing the assessment of unravel higher order structure of protein, epitope mapping and biopharmaceutical development. A big thanks to my whole team. https://lnkd.in/eKpNR-vE #Research #Science #Pharmacueticals #Biopharmaceuticals #Biotherapeutics #ProteinCharacterization #ResearchInnovation #HRPF #ProteinResearch #ScientificInnovation #ProteinStructure #StructuralBiology #Biochemistry #Proteomics #Biotechnology

Building the Future of E. coli Biomanufacturing Protein secretion is reshaping the way we think about biomanufacturing. At Vectron Biosolutions AS, we are leveraging this transformative technology to simplify production, improve efficiency, and expand the possibilities for creating high-value therapeutic peptides and proteins. Our latest article, "Building the Future of E. coli Biomanufacturing with Protein Secretion", dives deep into how our proprietary VB Secretion technology is making this transformation a reality. Read the full article here: Building the Future of E. coli Biomanufacturing https://lnkd.in/e5iqqi7h Why this matters: ✅ Simplified Purification: Proteins secreted directly into the extracellular media drastically reduce the complexity and cost of downstream processes. ✅ Efficiency at Scale: Our platform combines VB Secretion with VB Expression to enable scalable production of complex biomolecules. ✅ Accelerating Biopharma Timelines: These innovations are paving the way for faster, more cost-effective manufacturing of therapeutic peptides and proteins. Explore our journey and see how protein secretion is driving the future of E. coli biomanufacturing. Let us know your thoughts - how could this technology impact your work? #biotechnology #biomanufacturing #proteinsecretion #innovation #ecoli #therapeutics #sustainability

🤩 Have you downloaded a copy of the new application note on GlySERIAS™? GlySERIAS is a unique enzyme that digests flexible glycine-rich fusion protein linkers, enabling the separation of individual domains of multi-functional fusion proteins. This facilitates their characterization and enhances our understanding of these complex molecules 😎 In this new application note, we explore the preference of GlySERIAS for certain digestion sites, patterns, and kinetics of glycine-rich linkers, and it provides data that can aid in selecting experimental parameters tailored to your specific application and linker design! 📥 Download your copy of the application note here: https://lnkd.in/eX5xAcDJ #SmartEnzymes #MassSpectrometry #MiddleLevel #FusionProteins #GlySERIAS #ApplicationNote #AppNote #Biotech #Biopharma #Biopharmaceuticals