We found 3 key factors determinant for efficient TALE base editing ✂ First, what are TALE base editors? TALE base editors are a recent and important addition to the gene editing landscape. By design, TALE base editors do not create break within DNA strands as does CRISPR/Cas9, or other engineered nucleases. They represent a promising therapeutic strategy for genetic diseases. Interesting, right? But, to broaden the scope of possible applications, we need to understand the design rules. TALE base editors rely on the deamination of cytidines within double strand DNA, leading to the formation of an uracil (U) intermediate. These molecular tools are fusions of transcription activator-like effector domains (TALE) for specific DNA sequence binding, split-DddA deaminase halves that will, upon catalytic domain reconstitution, initiate the conversion of a cytosine (C) to a thymine (T), and an uracil glycosylase inhibitor (UGI). Previous works have pointed towards the positioning of targeted cytosine to be a key determinant for efficient editing. That’s why we investigated whether the nature (length and composition) of the linker that connects the TALE array with the split deaminase catalytic heads could impact C-to-T conversion within the editing window. We found 3 key factors: 💡 Spacer length 💡TALEB architecture 💡Composition of surrounding bases All of them can impact editing outcomes and further improve our understanding of TALE base editors' activity and specificity, leading to the possibility to tune and control editing using educated designs. Any thoughts on this? Drop them in the comments ⤵ For those interested in gene editing advancements, the full paper is available in Scientific Reports : https://lnkd.in/eSRZxyfN #GeneEditing #MolecularBiology #Genetics #BiotechResearch
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𝐑𝐞𝐬𝐞𝐚𝐫𝐜𝐡 𝐧𝐞𝐰𝐬 𝐟𝐫𝐨𝐦 𝐠𝐞𝐧𝐞𝐗𝐩𝐥𝐚𝐢𝐧! "𝐔𝐧𝐥𝐨𝐜𝐤𝐢𝐧𝐠 𝐃𝐢𝐚𝐛𝐞𝐭𝐞𝐬 𝐈𝐧𝐬𝐢𝐠𝐡𝐭𝐬: Research Utilizes TRANSFAC® for Gene Expression Analysis in pancreas" We're proud to share that our #TRANSFAC® database was instrumental in a recent study titled "𝐆𝐞𝐧𝐞 𝐞𝐱𝐩𝐫𝐞𝐬𝐬𝐢𝐨𝐧 𝐚𝐧𝐚𝐥𝐲𝐬𝐢𝐬 𝐫𝐞𝐯𝐞𝐚𝐥𝐬 𝐝𝐢𝐚𝐛𝐞𝐭𝐞𝐬-𝐫𝐞𝐥𝐚𝐭𝐞𝐝 𝐠𝐞𝐧𝐞 𝐬𝐢𝐠𝐧𝐚𝐭𝐮𝐫𝐞𝐬" The researchers applied bioinformatics to pancreas gene expression. By identifying transcription factor binding sites in the promoters of differentially expressed genes, the authors showcased that TRANSFAC® is highly effective in innovative discoveries. Kudos to the research team for their outstanding work! We're thrilled to support advancements that pave the way for better understanding and managing diabetes. Springer Nature Group University of California, Davis 𝐑𝐞𝐚𝐝 𝐩𝐚𝐩𝐞𝐫 𝐡𝐞𝐫𝐞: https://lnkd.in/eJhuA3h8 #Bioinformatics #GeneExpression #DiabetesResearch #TRANSFAC #geneXplain #InnovationInScience #GeneRegulation #Bioinformatics #ScientificInnovation #ProudPartner #collab #Transcriptionfactors #researchupdates #scientificcuriosity #bioinformaticstools #markets #genomeenhancer #scientificinterest
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TRANSFAC with MATCH Suite, TRANSPATH, HumanPSD, Software for transcription factor binding site search, promoter analysis, systems biology, bioinformatics
#TRANSFAC is instrumental to identify gene signatures in expression data
𝐑𝐞𝐬𝐞𝐚𝐫𝐜𝐡 𝐧𝐞𝐰𝐬 𝐟𝐫𝐨𝐦 𝐠𝐞𝐧𝐞𝐗𝐩𝐥𝐚𝐢𝐧! "𝐔𝐧𝐥𝐨𝐜𝐤𝐢𝐧𝐠 𝐃𝐢𝐚𝐛𝐞𝐭𝐞𝐬 𝐈𝐧𝐬𝐢𝐠𝐡𝐭𝐬: Research Utilizes TRANSFAC® for Gene Expression Analysis in pancreas" We're proud to share that our #TRANSFAC® database was instrumental in a recent study titled "𝐆𝐞𝐧𝐞 𝐞𝐱𝐩𝐫𝐞𝐬𝐬𝐢𝐨𝐧 𝐚𝐧𝐚𝐥𝐲𝐬𝐢𝐬 𝐫𝐞𝐯𝐞𝐚𝐥𝐬 𝐝𝐢𝐚𝐛𝐞𝐭𝐞𝐬-𝐫𝐞𝐥𝐚𝐭𝐞𝐝 𝐠𝐞𝐧𝐞 𝐬𝐢𝐠𝐧𝐚𝐭𝐮𝐫𝐞𝐬" The researchers applied bioinformatics to pancreas gene expression. By identifying transcription factor binding sites in the promoters of differentially expressed genes, the authors showcased that TRANSFAC® is highly effective in innovative discoveries. Kudos to the research team for their outstanding work! We're thrilled to support advancements that pave the way for better understanding and managing diabetes. Springer Nature Group University of California, Davis 𝐑𝐞𝐚𝐝 𝐩𝐚𝐩𝐞𝐫 𝐡𝐞𝐫𝐞: https://lnkd.in/eJhuA3h8 #Bioinformatics #GeneExpression #DiabetesResearch #TRANSFAC #geneXplain #InnovationInScience #GeneRegulation #Bioinformatics #ScientificInnovation #ProudPartner #collab #Transcriptionfactors #researchupdates #scientificcuriosity #bioinformaticstools #markets #genomeenhancer #scientificinterest
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New Layer of Human Gene Regulation Found. Researchers say they have developed a novel technique which, they claim, can determine for the first time how frequently, and exactly where, a molecular event called “backtracking” occurs throughout the genome of any species. The results of their study “Persistence of backtracking by human RNA polymerase II,” which appears in Molecular Cell, support the theory that backtracking represents a widespread form of gene regulation, which influences thousands of human genes, including many involved in basic life processes like cell division and development in the womb, according to the team led by scientists at the NYU Grossman School of Medicine. Read: https://lnkd.in/eJbAv6bv #sciencesdelavie #biotechnologie #biotechnology #innovation #innovations #recherche #sciences #medicaments #research #drugresearch #laboratories #labs #chemistry #chimie #biology #biologie #biopharma #rna #rnatherapeutics #arn #wewillwin
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Preimplantation Genetic Testing Market worth $1.2 billion by 2028 Download PDF Brochure:-https://lnkd.in/dKmKV7yb The size of global preimplantation genetic testing market in terms of revenue was estimated to be worth $0.7 billion in 2023 and is poised to reach $1.2 billion by 2028, growing at a CAGR of 11.4% from 2023 to 2028. 𝐓𝐡𝐞 𝐜𝐨𝐦𝐩𝐚𝐧𝐢𝐞𝐬 𝐟𝐞𝐚𝐭𝐮𝐫𝐞𝐝 𝐢𝐧 𝐭𝐡𝐢𝐬 𝐫𝐞𝐩𝐨𝐫𝐭 𝐢𝐧𝐜𝐥𝐮𝐝𝐞 Illumina, (US), Thermo Fisher Scientific (US), Agilent Technologies, (US), Revvity (US), and The Cooper Companies, Inc. (US). 𝐎𝐭𝐡𝐞𝐫 𝐜𝐨𝐦𝐩𝐚𝐧𝐢𝐞𝐬 𝐭𝐡𝐚𝐭 𝐚𝐫𝐞 𝐨𝐩𝐞𝐫𝐚𝐭𝐢𝐧𝐠 𝐢𝐧 𝐭𝐡𝐢𝐬 𝐦𝐚𝐫𝐤𝐞𝐭 𝐠𝐥𝐨𝐛𝐚𝐥𝐥𝐲 Natera, OXFORD GENE TECHNOLOGY LIMITED Beijing Institute of Genomics CooperSurgical Reprogenetics, LLC (Preimplantation Genetic Diagnosis) Genomic Prediction Recombine Biotech Genea Biomedx Sengenics Igenomix Yikon Genomics Genesis Genetics Invitae CooperGenomics OvaGene Oncology Yourgene Health The Genetics Company Progenesis Verinata Health, Inc. an Illumina Company Fulgent Genetics Phosphorus Sema4 Good Start Genetics Genetika Co. NextGen Jane, Inc. Genomicare Aspira Women's Health Diagnomics Laboratory Corporation of America Holdings Myriad Genetics, PACIFIC BIOSCIENCES OF CALIF BGI Genomics Fertility Associates Virtus Health Protean BioDiagnostics Caris Life Sciences Amplexa Genetics A/S #preimplantationtesting #geneticscreening #ivf #reproductivegenetics #pgt
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New Layer of Human Gene Regulation Found. Researchers say they have developed a novel technique which, they claim, can determine for the first time how frequently, and exactly where, a molecular event called “backtracking” occurs throughout the genome of any species. The results of their study “Persistence of backtracking by human RNA polymerase II,” which appears in Molecular Cell, support the theory that backtracking represents a widespread form of gene regulation, which influences thousands of human genes, including many involved in basic life processes like cell division and development in the womb, according to the team led by scientists at the NYU Grossman School of Medicine. Read: https://lnkd.in/e7wrk4bM #sciencesdelavie #biotechnologie #biotechnology #innovation #innovations #recherche #sciences #medicaments #research #drugresearch #laboratories #labs #chemistry #chimie #biology #biologie #biopharma #rna #rnatherapeutics #arn #wewillwin
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During recent years, more and more science has been emerging around #translatomics. As a step of gene expression, it is of the utmost importance to study translation to better understand why some genes are more expressed than others. Highly expressed genes often exhibit codon bias, matching quickly translated codons with abundant tRNAs. Using Immagina’s RiboLace + LaceSeq technologies and Quantitative Mature tRNA sequencing (QuantM-tRNAseq), Goldkamp et al https://zurl.co/qrMH investigated translation and tRNA expression across bovine tissues. They identified transcriptional and translational modulation of genes linked to tissue-specific processes, and defined pausing sites that influence translation rate and protein folding. A great example of how Immagina can support translatomic studies! Make sure to visit https://zurl.co/raRZ to find out more about our technologies and how they can support your research #GeneExpression #TranslationRegulation #tRNA #ProteinSynthesis #MolecularBiology
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Creativity is as important as knowledge / Director, Ph.D. Program in Sciences and Innovation in Medicine at Universidad del Desarrollo
Expanding the gene editing toolkit: Programmable RNA-guided (RNA-bridge) recombinases for genome editing Recombinases are enzymes that cut, flip, or insert DNA segments. Traditional ones recognize target sequences directly, making them difficult to modify. New research reveals recombinases that use RNA molecules as guides. These RNAs have customizable loops, called bridge RNAs, that bind to specific DNA sequences, allowing to design recombinases for precise DNA manipulations. The original articles were published in Nature, in addition to this commentary https://lnkd.in/ewJVnf-H #genetics #genomics #precisionmedicine #genomicmedicine #geneediting #rna #dna #genetherapy #molecularbiology #therapeutics #biotechnology #innovation #research #science #sciencecommunication
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🧬 New Frontier in Omics Analysis: ChIP-DIP 🧬 Detailed in a recent bioRxiv paper (https://buff.ly/4bgyffl) and featured on CBIRT (https://buff.ly/3OkiSsm), ChIP-DIP (ChIP Done In Parallel) is a split-pool-based method allowing for the simultaneous mapping of hundreds of regulatory proteins across the genome in a single experiment. 🧪 What Sets ChIP-DIP Apart? 🟢 Maps all classes of DNA-associated proteins, including transcription factors, RNA polymerases, chromatin regulators, and histone modifications. 🟢 Produces highly accurate protein maps, surpassing traditional methods that focus on one protein at a time. 🟢 Enables comprehensive understanding of gene regulation by mapping numerous proteins simultaneously. 🔍 Applications and Potential: 🔵 ChIP-DIP is invaluable for mapping DNA-associated proteins, using a unique approach of coupling antibodies to beads with distinct oligonucleotide tags. 🔵 The method opens doors for large-scale mapping of protein localization, crucial for decoding gene regulation and understanding genetic variations linked to human disease. 🔵 It can be integrated with existing split-pool approaches, enhancing capabilities like mapping 3D genome structures. 📢 Join the Conversation 📢 To my fellow biologists, bioinformaticians, and pharma leaders, let's discuss how ChIP-DIP can transform our understanding of gene expression regulation. Share your thoughts, ideas, and tools in the comments! 👇 💬 #GeneExpression #ChIPDIP #MolecularBiology #Bioinformatics #PharmaInnovation #Genomics #DNAProteinMapping
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Are your samples subject to degrading treatment? Extracting gene expression profiles and discovering mutations from FFPE tissues can often be a complex task due to the degraded and chemically modified RNA. But at SeqMatic, we are up to the challenge. We offer a superior RNA-seq service, designed to meticulously handle low-quality samples and extract the best possible data. Our process is guided by stringent sample quality controls and thorough library QC at every stage of the sequencing library generation. Also, the #SeqMatic #bioinformatics pipeline is optimized to detect artifacts, filter out contaminants, evaluate gene coverage uniformity, and measure the reproducibility of gene expression profiles among biological replicates. We're ready for any challenge, confident in the quality of our work, and eager to assist with your research. Go ahead and put us to the test - we can handle it! https://lnkd.in/g3945v8D #difficultsamples #histology #RNAseq #FFPE #CRO
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