ChemoMetec’s Post

The date is approaching! 📆 In a few days the Syngoi Technologies team will be in #Frankfurt at the 2nd edition of the mRNA Process Development and Manufacturing Conference. Julen Oyarzabal will give a session on the implementation of #innovations to improve the starting material. As well as on improved and advanced #mRNA manufacturing. We look forward to seeing you! #celltherapy #biotech #manufacturingmRNA #chemicalbiology

In this paper the authors present their work to develop a soft sensor, using oxgen uptake rates, for predicting viable cell concentration. #bioprocessing #cho #cellculture #bioreactor #mammaliancellculture https://lnkd.in/ee5Y5-rs

Join another fantastic webinar hosted by Technology Networks & Bruker Daltonics: “Enhanced Hit Quality in High-Throughput Protein–Ligand Interaction Screening” 👇 Register today to gain free access of which you can also refer back to ondemand #webinar #Highthroughputscreening #drugdiscovery #sciencemarketing

When choosing a CDMO for spray drying, it is critical to choose a CDMO with extensive particle engineering experience and proven processes to deliver successful spray-dried drug products. Serán BioScience has decades of experience in particle engineering, including spray drying. Our technology enables delivery of drugs to a wide variety of biological targets, including the small intestine to enhance bioavailability, colonic, and pulmonary delivery. We understand your unique needs and provide tailored solutions that deliver results. https://hubs.li/Q02DC_7V0 #drugdevelopment #spraydrying #amorphousdispersion #ASD #CDMO

While fluorescent labeling of EVs is quite a preferred (and necessary) practice for many research areas including diagnostics and therapeutics, traditional way of labeling comes with certain limitations including non-specific binding, stability issues, and photobleaching of fluorophores. However at HansaBioMed Life Sciences Ltd, we provide EV researchers with naturally-fluorescent EVs, secreted from engineered cells. Thanks to this cell enineering, our FLUO-EVs express EGFP (green), BFP (blue), or mCherry (red) as fusion proteins with CD9, CD63, and CD81. As a result, these FLUO-EVs do not bleach, stay stable, and do not possess the risk of measuring non-specific binding. Moreover, owing to being provided in lyophilized form, they are suitable for long term storage. The uniqueness of this product makes it special for in-vitro tracking and EV uptake monitoring studies, assay/instrument calibration purposes, as well as as positive controls for EV analyzers. If you are interested to learn more about FLUO-EVs, please do not hesitate to drop me a message! #extracellularvesicles #exosomes #EVs #fluorescentlabeling

🔬 Delving into the intricacies of therapeutic oligonucleotides (ONs), this study sheds light on the critical role of higher-order structures in the separation of ON diastereomers using HILIC. The research reveals how factors like column temperature and mobile-phase ionic strength can be fine-tuned to either enhance or suppress diastereomer separation, significantly advancing the characterization of PS-containing ONs. 𝐊𝐞𝐲 𝐭𝐚𝐤𝐞-𝐚𝐰𝐚𝐲𝐬: 📌 The study identified higher-order structures as the major cause of ON diastereomer separation in HILIC. 📌 It demonstrated how chromatographic settings, such as column temperature and mobile-phase ionic strength, can be adjusted to either enhance or suppress diastereomer separation. 📌 The research provided valuable insights into the application of HILIC for monitoring batch-to-batch variations in siRNAs. 📌 It showed that different ON designs, chemical modifications, and structural conformations significantly impact diastereomer separation. #Oligonucleotides #Chromatography #PharmaceuticalResearch #AnalyticalChemistry #GeneTherapy #RNAi #DrugDevelopment #Biotechnology Honorine Lardeux, Kathrin Stavenhagen, Clément PARIS, Rikke Dueholm, Camille Kurek, Leonardo De Maria, Felix Gnerlich, Tomas Leek, Werngard Czechtizky, Davy GUILLARME, and Manasses Jora

It’s frustrating for your research that a wealth of public experimental data exists, without standardization and meaning. Check out our webinar to learn how to overcome this by leveraging the synergy between QIAGEN Biomedical Knowledge Base #BKB and QIAGEN OmicSoft Lands to fuel your drug discovery programs. #drugdiscovery #bioinformatics ➡️ https://lnkd.in/efYAqghX

Expamers for T Cell Activation and Next Gen CAR-T Have you heard? Expamers are new drug substances being used to prepare a next generation of CAR-T. They are modular complexes that simultaneously "touch" and activate multiple cell receptors. 📔 Here's a paper by Mateusz Poltorak while he was a lead investigator at Bristol Myers Squibb. It was just shared with me the other day, and I was eager to check it out: https://lnkd.in/edCEQW_t - Expamers are ~100 nm complexes made of a Strep-Tactin backbone and anti-CD3/CD28 Ab fragments. - The backbone is polymeric and the Fab domains are tagged with a Strep-tag, allowing them to self-assemble. - They’re soluble and distribute evenly in cultures. Activation is stopped by disruption with biotin. They can be adapted for various T cell formats. - Expamers enhance T cell manufacturing efficiency and control, paving the way for advanced immunotherapies. #HPLC might help #CMC groups to test Expamers as critical drug substance materials #GTxResolve #biotech #CGT waters.com/CGT

🔬 The Power of IEX + Peptide Mapping The analytical teams at Regeneron have published yet another extraordinary paper! This time they delved into #AAV charge variant species on anion exchange and have applied a Lys-C & urea-facilitated one-pot digestion with CSH + formic acid peptide LC-MS to investigate the shift in charge properties. 🌡️ Thermal Stress Studies - AAV1 and AAV8 exhibit unique degradation patterns when exposed to heat; it is critical to understand this to optimize storage and shelf life. - AAV8 gives an acidic shift due to N263 deamidation. - AAV1 displays a basic shift primarily due to D328 succinimides/isomerization. 💡Implications: These insights are vital for developing robust AAV-based therapies by enhancing the understanding of stability and degradation mechanisms. 📑 Open Access paper in the Journal of Pharmaceutical and Biomedical Analysis: https://lnkd.in/e7vqBzun #AAV #GeneTherapy #AnalyticalChemistry #HPLC #LCMS #MassSpec #Biotech #Biotechnology #CGT Authors: TAO XING, Shuai Li, Yu H., Gaoyuan Lu, Yuetian Yan, Dingjiang (Dean) Liu, shunhai wang, Li Zhi, Mohammed Shameem, Ning Li

If you're working with cell counting, monoclonal cell isolation, or cell expansion, you should definitely check out our 3D workflow, which integrates three powerful technologies: the CASY Cell Counter and Analyzer, the CellRaft AIR System, and the CERO 3D Incubator & Bioreactor. These tools are designed to streamline your research and deliver consistent, reliable results. For more details, visit our website at https://lnkd.in/edgNZjJ7 #stemcells #biosciences #biotechnology #precisionmedicine