ChomiX Biotech’s Post

Photoaffinity labeling (PAL) using a #chemical probe to covalently bind its target in response to activation by light has become a frequently used tool in #drugdiscovery. Based on PAL, ChomiX has developed a technical platform for chemoproteomic profiling protein targets for noncovalent small molecule drugs. Non-covalent interactions, such as hydrogen bonds and π-π stacking, can be disrupted due to #protein denaturation. To address this challenge, our platform employs photoaffinity labeling to precisely attaching "chemical labels" to a protein's active site. Furthermore, our innovative in situ chemical crosslinking strategy transforms transient non-covalent protein interactions into covalent and permanent chemical bonds. By utilizing a chemical probe that is functionalized with both photoaffinity and bioorthogonal moieties, ChomiX's chemoproteomics platform has demonstrated its effectiveness in successfully fishing out protein targets within cell lysates, tissues, and living cells. The spectrum of bioactive small molecule drugs applied in the platform encompasses a variety of compounds, including endogenous metabolites, #naturalproducts, and non-covalent synthetic molecules.