Derrick Ong’s Post

#researchadvances Raman microscopy reports on the vibrational energy levels of proteins and metabolites in a label-free and nondestructive manner at subcellular spatial resolution, but it lacks genetic and molecular interpretability. A recent paper presents Raman2RNA (R2R), a method to infer single-cell expression profiles in live cells through label-free hyperspectral Raman microscopy images and domain translation. With live-cell tracking of mouse embryonic stem cell differentiation, R2R traced the early emergence of lineage divergence and differentiation trajectories, overcoming discontinuities in expression space. R2R lays a foundation for future exploration of live genomic dynamics. Read the paper here: https://lnkd.in/ev22V74N Aviv Regev Jian Shu Tommaso Biancalani Koseki K. #ramanmicroscopy #protein #R2Rmethod #singlecellRNA #biologyfront #newpaper #biologyresearch

Recent progress in chemistry, molecular and cell biology, and nanotechnology has expanded the strength of #MRI to characterize pathological tissue changes by providing tools that visualize cellular and molecular processes. A wealth of novel imaging probes, reporter gene - and cell-labelling strategies provide the basis for gaining unprecedented insight into the pathomechanisms of neurological disorders. Here, we illustrate examples from academic research groups’ work on the application of molecular and cellular MRI in models of neurological disorders and its use for the evaluation of gene and cell therapies. Download the paper here: https://lnkd.in/dt_E28yN Please visit us at booth C08 at the ISMRM Conference on May 4-9 in Singapore to learn more about Bruker's MRI solutions: https://lnkd.in/dwggq4q7 #ISMRM #ISMRM2024 #Imaging #PreclinicalImaging #Neurology

🚀 Breaking New Ground in Spatial Multi-Omics! 🧬 Exciting advancements in #Biotechnology! A team led by Xiaofeng Wu and colleagues has developed MiP-seq (multi-omics in situ pairwise sequencing), a revolutionary method for detecting DNAs, RNAs, proteins, and biomolecules at subcellular resolution. 🌟 Key Highlights: - Enhanced Decoding Capacity: Compared to other methods, MiP-seq boosts decoding capacity while slashing sequencing and imaging costs. 📉 - Comprehensive Detection: Effectively detects gene mutations, allele-specific expression, and RNA modifications. 🧬 - Integration with Advanced Imaging: Compatible with in vivo calcium imaging and Raman imaging, enabling the creation of a spatial multi-omics atlas of mouse brain tissues. 🧠 - Innovative Dilution Strategy: Resolves optically crowded signals during in situ sequencing. 🎨 This high-throughput approach is set to revolutionise the multidimensional analysis of molecular and functional maps of tissues, paving the way for groundbreaking discoveries in cellular functions and disease mechanisms. 🔬 #MultiOmics #MiPseq #BiotechInnovation #SpatialOmics #Genomics #Proteomics #Neuroscience #BiomedicalEngineering #ResearchBreakthrough #ScienceInnovation

The complexity of life is far from our computational knowledge to model its details. Please take a look at the recent paper in "Frontiers in Cell and Developmental Biology". https://lnkd.in/dKigrxGM This paper cited our recent paper entitled, " A Shadow of Knowledge in Stem Cell Science". https://lnkd.in/d-s6_mAy

🚀 Emerging Research Fields You Need to Know! 🚀 Explore the frontiers of science with these top areas in demand: 🔬 Immunology: Immunotherapy, Microbiome Immunology, Personalized Immunology 🧠 Neuroscience: Neural Circuits, Neurodevelopmental Disorders, Neural Plasticity 🧬 CRISPR & Gene Therapy: Transformative innovations 🔧 Bioprocess & Manufacturing: AI & ML in Bioprocess, Continuous Manufacturing 💡 Bioinformatics: Single-cell Genomics, Multi-omics Integration, AI-ML in Bioinformatics 💊 Drug Discovery: AI-driven insights, Drug Repurposing Join the revolution at www.biotecnika.org where knowledge meets innovation. 😇 A Place where We Help your dreams take flight ✈️ #ResearchTrends #Biotech #Immunology #Neuroscience #CRISPR #Bioinformatics #DrugDiscovery

Today we feature a recent publication from the group of Prof Melike Lakadamyali from the University of Pennsylvania Perelman School of Medicine. Their paper, titled "Dissecting gene activation and chromatin remodeling dynamics in single human cells undergoing reprogramming", addresses the fine-tuned regulation of induced pluripotent stem cell reprogramming. In 2006, Prof Shinya Yamanaka changed the face of biological #research when his team discovered that with the correct regulation, only four proteins are enough to transform mature skin cells into embryonic-like pluripotent cells, which regain the potential to differentiate into any other mature cell. For this discovery, he was awarded The Nobel Prize in Physiology or Medicine in 2012, which he shared with Sir John B. Gurdon. To this day, the question of how exactly these 4 proteins reverse cellular differentiation remains unanswered. Some of the challenges arise from the fact that a bulk of many cells is heterogeneous, and it is very difficult to determine the exact sequence of events in each cell. #SuperResolution #microscopy allows scientists to monitor events at a single-cell level, therefore obtaining an accurate description of what happens when. Prof Lakadamyali and her team used the #Nanoimager for several different #SingleMolecule approaches, namely #STORM and smRNA-FISH, in order to study the dynamics of RNA expression and chromatin state. Their work reveals that the regulation of these events is intricate and complexed, and above all, is gene-specific. Further in-depth analysis in real-time could characterize the exact sequence of events that truly leads cells back to embryonic-like state. Read their full paper here: https://hubs.li/Q02xy7Tl0 #SMLM #dSTORM #smFISH #RNAFISH #OligoSTORM #biology #LifeSciences #STEMcells #MolecularBiology #Biophysics #innovation #imaging #microscope #pluripotentCells #iPSC

#road2DAUINPHDDAY ✈ In view of the next edition of the DAUIN PhD Day (November 2024), we present the research activity of the PhD students in Computer and Control Engineering - 37th and 38th cycles. Join the PhD Day to discuss with them at poster sessions! 👉 Lorenzo Martini: Complex systems science and brain dynamics Many people may remember the phrase “DNA is the blueprint of life.” But if all human cells share the same blueprint, where does the incredible cell diversity come from? The answer lies in specific gene expression profiles and the complex system of transcriptional regulation. My work focuses on creating computational tools and bioinformatic workflows to investigate and integrate cutting-edge experimental data to shed light on brain dynamics process and cancer research. 🔗 https://lnkd.in/ekaeCGKZ

🌟 Discovering the Miracles of Regeneration: Planaria 🌟 Planaria, a type of flatworm, possess an extraordinary ability to regenerate. When cut into pieces, each fragment can transform into a complete organism. This remarkable potential is driven by their pluripotent stem cells called neoblasts, which can become any cell type required for regeneration. The process involves intricate wound healing, cell proliferation, differentiation, and patterning, guided by a sophisticated network of genes and signaling pathways. Studying planarian regeneration not only deepens our understanding of stem cell biology but also opens new horizons in regenerative medicine and tissue repair. 🔬🧬 #RegenerativeMedicine #StemCellResearch #Biology #Innovation #Science #Healthcare #MedicalResearch

The graphic design in this research is very clear and effective in descriving complex phenotypes and organ anatomy. Great job #broadinstitute!!! #graphicdesign

Searching for more fields to expand the research for CJD, I reached Prof. Gustavo Mostoslavsky years ago. Gustavo is an expert in stem cell research and a Co-Director at the Center for Regenerative Medicine (CReM) at Boston University. He created #iPSCs derived neurons from our family's samples with genetic CJD, as a model that can provide important mechanistic insights and a powerful platform for testing candidate #therapeutics. I'm so excited to see this important research realized and published and to get this meaningful message from Gustavo, adding my name as a co-author: "Alice, this is really a statement for all your hard work. I really mean it when I say that none of this would have happened if it wasn’t for you and your constant devotion for moving forward the research in CJD. Hopefully this is only the beginning of many more to come!!" As a proactive carrier of this horrible fatal #neurodegenerative #genetic disease, advancing #research has become my passion and hope. I founded a #biobank, knowing that our samples can advance research for CJD and make a real difference! For the full article: https://lnkd.in/evwqpaWG