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Jan. 12 Symposium “Bits, Photons & Cells Symposium” - Registration for virtual attendance is still open for a few more days.   Bits, Photons & Cells Symposium Friday, January 12, 2024 | 9:00 a.m.–6:00 p.m. at Chan Zuckerberg Biohub – San Francisco From omics to microscopy, technological innovation is pushing the limits of observability, allowing us to delve into previously inaccessible insights, thus reshaping our understanding of how cells form organs and tissues. This symposium aims to be a forum to discuss these advances, providing a platform for dialogue, collaboration, and discovery. The “Bits, Photons & Cells” symposium aims to gather like-minded scientists passionate about creating and applying state-of-the-art technologies and methods. Our focus is on the emergence of cell identity in zebrafish, but the implications reach far beyond this model organism. https://lnkd.in/eTveyCui

We've spent a lot of time during my PhD looking into the issue of extracting high resolution images from a complex sample that is a liver-on-a-chip. We came up with multi-modal labelled and label-free microscopy approaches (2PEF, SHG, THG, SRS) which revealed: a) Distribution of fluorescent anti-sense oligonucleotides (ASOs) from deeeeep within a chip (>150 microns) and confirmed the positive effect of GalNAc moiety on ASO hepatocyte uptake dynamics. b) Intracellular ASO trafficking is likely dependent on hepatocyte morphology. c) Chemical components associated with hepatocyte morphology were discovered potentially making hepatocyte classification and segmentation easier in future studies. This has been recently published in a research paper with the Lab-on-a-Chip journal. https://lnkd.in/gymcgweU Big thanks to everyone involved in the paper, especially to Aneesh Alex

We are heading to the WMIC meeting next week and are ready for Montreal! Drop by our booth; we've got lots of great info on the Vevo F2 LAZR-X, molecular imaging and contrast agents. #seeingmorematters #visualsonics #WMIC2024 #Montreal #highfrequency #ultrasound #imaging #research #science #molecularimaging

👋 Introducing Prof. Dr. Markus Sauer, a visionary Jury Member of the #GlowingLifeChallenge and the co-founder of ATTO-TEC GmbH. As a Professor of Biotechnology and Biophysics at Julius-Maximilians-University of Würzburg, Prof. Sauer brings his 25 years of experience in developing efficient dyes for high-sensitivity fluorescence microscopy and pioneering single-molecule fluorescence methods as well as super-resolution microscopy techniques.   👨🔬 Prof. Dr. Sauer, what drives your passion for fluorescence microscopy?   "Super-resolution imaging unveils the intricate architecture and workings of cells at an unmatched level of detail, down to individual molecules. Its impact on our grasp of cellular processes, especially in tracking infections and enhancing immunotherapies, is huge. We're not just doing science—it's like we're unlocking a whole new dimension of life."   👨🔬 Any tips for our participants? "For those looking for inspiration, I recommend checking out #FluorescenceFriday on platform X. It's a treasure trove of stunning examples that perfectly blend scientific insight with visual impact.   📸 Submit your high-resolution insights into the microscopic world and take part in shaping the future of personalized immunotherapies! https://lnkd.in/eJH_3cFn   #MeetTheJury #LifeSciences #FluorescenceMicroscopy #attotec

Revolutionizing our understanding of muscle mechanics, researchers have finally captured the elusive pre-stroke state of the myosin protein, a key player in muscle contraction, using advanced cryo-electron microscopy. This breakthrough, detailed in a recent study published in Nature Portfolio, validates theories posited since the 1950s and opens new avenues in biomedical research. Ewen Calloway's article describes how cutting-edge technology transforms our ability to visualize molecular processes in motion, promising new insights into cellular functions and medical treatments. #MuscleMechanics #StructuralBiology #CryoEM

It’s #WavelengthWednesday! So we continue our educational series this time delving into the realm of multiphoton microscopy: “Why do shorter pulses matter?” 🔬 In multiphoton microscopy, the significance of shorter pulse durations cannot be overstated, as Oliver Prochnow explains in this video.   📌 Picture this: in multi-photon applications, the aim is to have two or three photons converge simultaneously at the same spatial location.   📌 Now, imagine the focal point where photons converge in abundance. With a shorter pulse duration, the influx of photons intensifies within a brief timeframe, significantly enhancing the likelihood of triggering a multi-photon absorption event. This heightened efficiency translates to improved fluorescence detection, unlike the autofocus fluorescence seen in single photon excitation.   📌 But that's not all. Shorter pulses also pave the way for deeper penetration, extending up to a millimeter into the sample. Moreover, they play a significant role in mitigating photobleaching and photodegradation, thereby prolonging cell viability and imaging longevity🧪⏳.   So, in essence….embrace shorter pulse durations in multiphoton microscopy to not only boosts efficiency but also extend the lifespan of cellular samples! As we say over here: “Long Live the Cell!” 🦠   #MultiphotonMicroscopy #LearnScience #Biotechnology #FluorescenceDetection  

**Exploring the Realm of Cryo-Scavenging: The Science Behind Preserving Life** Cryo-scavenging, a niche field within cryobiology, is revolutionizing our approach to cellular preservation. Unlike traditional cryopreservation which focuses on freezing cells, cryo-scavenging uses controlled thermal gradients to minimize ice formation and cellular damage. Recent breakthroughs involve employing antifreeze proteins and vitrification solutions to prevent ice crystal formation, enhancing viability during thawing. This technique has profound implications for organ transplants, cellular therapies, and extending the viability of biological samples. Researchers are now exploring its potential for preserving human tissue at the microscopic level, which could one day enable long-term space travel or advanced medical treatments. #CryoScavenging #CellularPreservation #BiologicalResearch

Cornell University researchers have developed an innovative Light-Sheet Photonic-Force Optical Coherence Elastography System that is poised to revolutionize the way we measure mechanical properties of viscoelastic materials. 🌟 This advanced system utilizes a focused light-sheet photonic force beam to manipulate micro-particles within materials, enabling non-destructive and in situ measurement. Equipped with a precise interferometry apparatus, it is capable of accurately measuring nano- to sub-nanometer vibrations, making it a valuable tool for industries such as materials sciences, medicine, and engineering. 🏥 In the medical field, it has the potential to aid in early-stage cancer diagnosis and provide quantitative data for physiological and pathological analysis. ✉️ For those interested in licensing or learning more about this groundbreaking technology, please contact our licensing team Jesús Garcia-Figueroa and Ryan Luebke. 🔗 https://ow.ly/f2PP50QyOcS #BiomedicalEngineering #Biomechanics #CornellResearch

Near-field microscopy is rapidly growing and evolving, and I’m happy to share with you the next step: s-SNOM and nano-FTIR in aqueous environments! Based on a nm-thin membrane approach pioneered by KJ Kaltnecker, F Keilmann et al. (CeNS & LMU), the AFM tip remains in air and the aqueous environment is “part of the sample”. Thus, from a technical POV the problem is solved, and now needs daring scientists to potentially open new research fields in #LifeScience, #Biology and others. Earlier this year we successfully delivered the first Liquid Cell Kit to AG Heberle & AG Kozuch at FU Berlin and demonstrated in-vivo nano-FTIR on site -> the zoom-in shows water absorption around 1600 cm-1. To clarify: this enables super-resolution nanoscopy of living objects, but label-free and with chemical sensitivity!   Please do reach out to us to learn all about this, and follow #fridaySNOM for more updates coming very soon. Exemplary data in comments section. Liquid cell developed together with #Norcada, flow control by #Fluigent. Center for NanoScience, LMU Munich Ludwig-Maximilians-Universität München Freie Universität Berlin (Free University of Berlin)

I'm in science to make incremental impacts to knowledge, which are then built upon by others. This week is an example of this. Seven years ago we showed that OCT could be used to quasi-instantaneously image the changes caused by osmotic flow in a cornea. https://lnkd.in/eZuKxVqU Matteo Frigelli and co, at ARTORG Center for Biomedical Engineering Research have now followed this up with a similar technique. They have then used it to demonstrate very clearly that the changes induced by corneal cross linking has a very measurable and immediate impact on osmosis processes within the cornea. I highly recomend their work: https://lnkd.in/eEF2CDBd