DNA Genotek’s Post

If you're interested in developing clinical tests based on next-generation sequencing (NGS), I suggest you watch the CLSI MM09 webinar, which is free of charge. This webinar covers the key stages of diagnostic test development using NGS. Additionally, it includes a wealth of links to various regulatory guidelines and relevant scientific literature. https://lnkd.in/d9Kh9aJ3

New preprint alert from my group 🚨 We provide insight into a novel and non-canonical function of DNA glycosylases Ogg1 and Mutyh in modulating the epigenome of PRC2 target genes in brain to promote memory formation. So pleased to see this story finally come together. https://lnkd.in/dgs2wTpn

I'm happy to share our latest NAR paper in the field of drug discovery against Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. We are presenting the first crystal structure of GGCCCC RNA in complex with the synthetic molecule ANP77. We determined that RNA folds into a triplex-like structure involving protonated cytosine residues, whereas ANP77 interacts directly with RNA via pseudo-canonical base pairs. These crystallographic models are rare examples demonstrating unexplored potential of cytosine-rich sequences to form complex RNA structures and provide sophisticated crystallographic templates for structure-guided approaches in the development of lead compounds for drug discovery against ALS/FTD.

Another very exciting work! DNA i-motifs or DNA knots [Dnots;) "hemi-protonated intercalated cytosine base pairs folded into a tetrameric structure"] are important regulatory elements spread across the genome (>50k, ~50% intergenic) and related with genes upregulated during early cell cycle phases. It will be fantastic to see results from DNA i-motifs in human primary cells and tissues, in healthy and various disease contexts as well as in regard to cell cycle control! 👌

Get early access to AVITI24 and see how deep multiomic profiling can transform your research. CytoProfiling analyzes DNA, RNA, proteins, and cell morphology simultaneously from a single sample. https://lnkd.in/drdXp29D

*Publication Alert* Hello LinkedIn family happy to share the latest review article of our group in the journal Molecular Therapy: Nucleic Acids. In this review we have discussed and highlighted the different roles of RNA modifications in disease-associated macrophages. Feel free to get in touch if you would like to know more about this topic! Jo Van Ginderachter Camille Huart

DNA sequencing has many steps – Revvity unites them all. Empower your NGS research with our brand new workflow. http://ms.spr.ly/6042YdBwK For research use only. Not for use in diagnostic procedures.

Multifaceted roles of RNA editing enzyme ADAR1 in innate immunity The latest article from the #RNASpecialIssue explores the genetic and mechanistic evidence for ADAR1’s multipronged role in suppressing dsRNA-mediated autoimmunity, and the evolving roles of ADAR1 as an immuno-oncology target. Access now: bit.ly/49Qf9e3 #RNAEpitranscriptomics #ADAR1

Exciting News: Our Inaugural Paper of 2024! Unraveling the intricate world of alternative splicing, a post-transcriptional process crucial for amplifying protein diversity. The orchestration of alternative splicing is wielded by RNA binding proteins, exhibiting various control mechanisms. However, amidst the myriad of predicted RNA binding proteins, a systematic evaluation of their direct involvement in enhancing exon inclusion remains unexplored. In our latest research study, we share our systematic assessment of hundreds of both established and predicted RNA binding proteins. The focus of our study revolves around discerning their potential to directly influence exon inclusion (by tethering). This undertaking marks a step forward in clarifying the network of proteins responsible for shaping the transcriptome. As an intriguing implication, we hint at the identification of promising candidates that could be seamlessly fused with RNA-targeting proteins. We find exon suppression is relatively trivial for RNA binding proteins (many modalities here) but this fusion holds the potential to cause exon inclusion over reporter and endogenous splicing events, offering a glimpse into the future possibilities of manipulating RNA processing with higher precision. Congrats newly minted alumni Jon Schmok and others from my lab! Thank you Marko Jovanovic and Lena Street for the collaboration. #RNA #RNAbindingproteins #splicing https://lnkd.in/gyDN4e-R

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