Understand column and chemistries choices so you can optimize your purification method and plan for scale-up. https://bit.ly/4bjdbDN #oligonucleotidepurification#oligonucleotide#oligo

Among the indispensable, low-molecular weight cyclic amines, piperidine scaffold serve as privileged core scaffold in medicinal chemistry, with substituted variants having a great number of potential regio- and diastereoisomers, which allows high stereochemical definition to enable a variety of productive protein interactions The work describes, the successful enablement, scale-up, and delivery of >400 g of a single isomer, (3S,5S)-1-((benzyloxy)carbonyl)-5-fluoropiperidine-3-carboxylic acid (>98% de and >96% ee), via 450 g-scale biocatalytic desymmetrization and 335 g-scale flow photochemical decarboxylative fluorination. https://lnkd.in/gqjPRWKn

Tackle challenges and limitation of traditional polishing steps to address high aggregate engineered MAb feedstocks. Join our Webinar to learn about ThermoFisherScientific's latest Mix mode Caprylate resin designed to remove moderate to high levels of aggregates during downstream processing of therapeutic antibodies in flow-through mode. #purification #mabs #complexmabs #bioprocessing #downstreamprocessing

Derivatization of carbonyl compounds with 2,4-dinitrophenylhydrazine and their subsequent determination by high-performance liquid chromatography

At Agilent, we recognize the diversity of the oligonucleotide landscape, and we offer the optimal chemistry for robust resolution, reproducibility, and long column lifetime. 🔬 Agilent AdvanceBio Oligonucleotide columns incorporate high-efficiency Poroshell particles, available in 2.7 µm and 4 μm sizes. These superficially porous particles undergo proprietary chemical modification, rendering them highly resistant to high pH mobile phases. This enhances separation and denaturing conditions, optimizing resolution for oligonucleotide analysis. The particles are bonded with an end-capped C18 phase, providing excellent selectivity for oligonucleotides and making them ideal for LC/UV and LC/MS characterization workflows. Find out more about Agilent Oligonucleotide Chromatography Solutions: https://lnkd.in/egvMVJRq #LC #MS #Chromatography #OligonucleotideAnalysis

Following our peptide macrocyclization work, we reported a multicomponent amine bioconjugation using the same sulfonyl chloride-derived reagent. With the utilization of a gas-phase reagent for a reaction mediated by metal ions, a variety of biologically relevant molecules, such as saccharide, PEG, azide, alkyne, fluorophore, and affinity tag, can be efficiently crosslinked to the N-terminus or lysine side chain amines on natural polypeptides or proteins. https://lnkd.in/g6aRzSxG

Accelerate your haloacetic acids, bromate, and dalapon analyses using an integrated Thermo Scientific ion chromatography tandem mass spectrometry (IC-MS/MS) workflow that is fully compliant with U.S. EPA Method 557. By eliminating the need for sample preparation through direct injection, this workflow not only saves valuable time, but also reduces costs significantly. http://spr.ly/6047ge7vc #ionchromatography #haloaceticacids #disinfectionbyproducts #dalapon #dionex

𝐓𝐂𝐈 𝐏𝐫𝐚𝐜𝐭𝐢𝐜𝐚𝐥 𝐄𝐱𝐚𝐦𝐩𝐥𝐞: 𝐓𝐡𝐞 𝐂𝐨𝐧𝐝𝐞𝐧𝐬𝐚𝐭𝐢𝐨𝐧 𝐑𝐞𝐚𝐜𝐭𝐢𝐨𝐧 𝐔𝐬𝐢𝐧𝐠 𝐚 𝐂𝐚𝐩𝐬𝐮𝐥𝐞 𝐑𝐞𝐚𝐠𝐞𝐧𝐭 We are proud to present the #condensationreaction of 4-bromobenzoic acid and morpholine using the capsule reagent. This reagent can omit weighing the condensing agents. 0.25 mmol of EDCI·HCl and 0.04 mmol of HOBt·H2O are encapsulated in each capsule. 𝐔𝐬𝐞𝐝 𝐂𝐡𝐞𝐦𝐢𝐜𝐚𝐥𝐬: 4-Bromobenzoic Acid [Product No-B0553] Morpholine [Product No-M0465] Reagents for Condensation Reactions (HPMC encapsulated) (EDCI·HCl, HOBt·H2O) [Product No-R0274] Water 𝐋𝐞𝐚𝐫𝐧 𝐌𝐨𝐫𝐞: https://lnkd.in/ggxjYwhg Andreja Matelič Nathalie Stupar Meriem Boulefa Charles E. Ocampo, Ph.D. Moritz Faust #tcieurope #tciproduct #laboratorychemicals #chemicalreagents #researchchemicals #industriallubricants #chemicalmanufacturer #europeanchemicals #researchreagent #sustainability #chemicalforresearch

Toward a Chemical Constructor: A Lego-Like Approach for Formal α-Alkylation of Cyclic Ketones A conceptual strategy for a formal α-alkylation of α-methylene ketones. Wide functional group tolerance ester, Boc-protected amine, and α-fluoro-substituted ketone moieties untouched. A well suited recipe for late-stage modification. Step 1: Synthesis of enaminone, introducing diverse 1° and 2° alkyl substituents in the α-position Step 2: Treatment with organomagnesium Step 3 and 4: Subsequent catalytic hydrogenation (1° alkyl) or organocopper reagents. https://lnkd.in/gmBwdda3

Week 2 of "𝗧𝗵𝗲 𝗣𝗿𝗼𝘁𝗲𝗶𝗻 𝗣𝘂𝗿𝗶𝗳𝗶𝗲𝗿: 𝗘𝘅𝗽𝗲𝗿𝘁 𝗜𝗻𝘀𝗶𝗴𝗵𝘁𝘀, 𝗜𝗻𝗯𝗼𝘅 𝗗𝗲𝗹𝗶𝘃𝗲𝗿𝘆". Every Friday, we're posting a new technical article that you can read here on LinkedIn or have delivered directly to your inbox 📩 This week's topic: "𝗖𝗼𝗻𝘃𝗲𝗿𝘀𝗶𝗼𝗻 𝗼𝗳 𝗶𝗼𝗻-𝗽𝗮𝗶𝗿𝗶𝗻𝗴 𝗮𝗴𝗲𝗻𝘁𝘀/𝗰𝗼𝘂𝗻𝘁𝗲𝗿 𝗶𝗼𝗻𝘀 𝗶𝗻 𝗽𝗲𝗽𝘁𝗶𝗱𝗲𝘀 𝗮𝗻𝗱 𝗼𝗹𝗶𝗴𝗼𝗻𝘂𝗰𝗹𝗲𝗼𝘁𝗶𝗱𝗲𝘀" Here's a summary of what you'll learn after reading it: ❓ Why ion-pairing agents are often used in RPC/IPC purification ➕ How cation exchange (CIEX) resins can be used to remove ion-pairing agents like trifluoroacetic acid (TFA) 📃 Various protocols for TFA removal using CIEX for peptides with lower pIs https://lnkd.in/daQCV84M Use the above link ☝️ to get a copy delivered to your inbox, and follow us to stay informed when we post the other technical articles in this series.