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This is today’s edition of The Download, our weekday newsletter that provides a daily dose of what’s going on in the world of technology. The many uses of mini-organs This week, we reported on a team of researchers who managed to grow lung, kidney, and intestinal organoids from fetal cells. Because these tiny 3D cell clusters mimic…

New connection between impaired autophagy and heart failure Autophagy is a natural, self-serving mechanism in which the body removes damaged or dysfunctional parts of a cell and recycles their components toward cellular repair or energy production. Scientists weren't sure what mechanisms underlie mitochondrial and cardiac dysfunction in the setting of impaired autophagy. Using a mouse model with autophagy dysfunction in heart cells, the research team found that autophagy regulates the enzyme NNMT, whose levels were increased in this heart failure model. Inhibiting the activity of this enzyme with a small molecule led to an improvement in heart failure even though autophagy failure persisted. The study elucidated a chain event that explains how and why impaired autophagy can lead to cardiac dysfunction. The first step of this chain is the accumulation of a protein known as SQSTM1. Increased SQSTM1 activates a signaling protein called NF-κB (also known as RELA). NF-κB enters the nucleus and causes increased activity of the gene that encodes the enzyme known as nicotinamide N-methyltransferase (NNMT). NNMT ultimately leads to a breakdown of NAD+ precursors, ultimately lowering NAD+ levels. Low NAD+ levels then cause mitochondrial and cardiac dysfunction. #ScienceMission #sciencenewshighlights https://lnkd.in/gBB5YX3i

Scientists use three-dimensional cell culture models, such as organoids, to replicate an organ’s functionality and structure in vitro. However, researchers with limited experience often find it challenging to develop organoid models. In this webinar brought to you by Sartorius, Aitor Aguirre, Ph.D. will explain how his group produces mini hearts and uses these organoids to investigate development and disease. Register today #AD: https://ow.ly/ksgt50RuaVU

Exploring how extracellular vesicle-depleted serum affects endothelial cell traits across time: Luiz Fernando Garcia, Pryscilla Wowk and Letusa Albrecht at FIOCRUZ - Fundação Oswaldo Cruz presented the first study comparing EV characteristics from human endothelial cells collected at two different culture times https://lnkd.in/gdJGp6Df Their findings revealed that human brain microvascular endothelial cells cultured with EV-depleted serum produce extracellular vesicles with evolving physical properties and varying protein levels over time #extracellularvesicles #exosomes #cellculture #proteomics #Vesiculab

🔸 Single-cell multiomics analysis of myelodysplastic syndromes and clinical response to hypomethylating therapy Campillo-Marcos et al. used single-cell technologies to simultaneously map the genome and immunoproteome of myelodysplastic syndromes (MDS) throughout clinical evolution. Key points 📍The architecture and evolution of molecular clones in precious paired bone marrow MDS samples at diagnosis and posttreatment 📍A combined imbalance of specific cell lineages with diverse mutational profiles associated with the clinical response to hypomethylating therapy. ➡ More details: https://lnkd.in/eTCF9u5P #spatialomics #spatialbiology #singlecellanalysis #singlecell

https://lnkd.in/g4uhbjGt how telomerase sparks new insights into ocular angiogenesis: our lastest research article uncovers the hidden connections

Small extracellular vesicles from young plasma reverse age-related functional declines by improving mitochondrial energy metabolism Recent studies on heterochronic parabiosis have revealed that small extracellular vesicles (sEVs) from young blood can rejuvenate aged tissues. 🔍 The latest research demonstrates: 💉 Intravenous injections of young sEVs extend the lifespan of aged mice. 🧬 These sEVs counter aging at molecular, mitochondrial, cellular, and physiological levels. 📈 They enhance PGC-1α expression through miRNA cargoes, improving mitochondrial function and reducing age-related declines. 🔬 Quantitative proteomics has shown significant changes in the proteomes of aged tissues, linked to metabolic improvements after sEV treatment. 🌐 This discovery opens new doors for potential therapies targeting aging and its associated diseases. From Nature aging: https://lnkd.in/gX9BeM4N #AgingResearch #Biotech #MitochondrialHealth #MedicalInnovation #ScienceNews

In this week’s #MAbMonday spotlight, we’d like to recognize the work on An Optimized Single-Molecule Pull-Down Assay for Quantification of Protein Phosphorylation. https://hubs.la/Q02bQzpX0 The team stated, "Phosphorylation is a necessary posttranslational modification that regulates protein function and directs cell signaling outcomes. Current methods to measure protein phosphorylation cannot preserve the heterogeneity in phosphorylation across individual proteins. The single-molecule pull-down (SiMPull) assay was developed to investigate the composition of macromolecular complexes via immunoprecipitation of proteins on a glass coverslip followed by single-molecule imaging." 👇Featured product👇 Anti-Human EGFR (External Domain) – Biotin: https://hubs.la/Q02bR3Mj0 #Leinco #ReproducibleScience #Immunology

Ding C, Lim YC, Chia SY, Arcinas C, Xu S, Lo KA , Zhao Y, Zhu D, Shan Z, Chen Q, Leow M, Xu D, and Sun L. De novo reconstruction of human adipose transcriptome reveals conserved lncRNAs as regulators of brown adipogenesis. Nature Communications, 2018 Apr 6;9(1):1329. doi: 10.1038/s41467-018-03754-3. Deep RNA-seq showed that 909 out of 3149 adipose active IncRNAs were detected in brown adipose tissue (BAT). In addition to this, Scientist found that IncRNAs regulate nearby mRNAs. And Inc-dPrdm16 impaired brown adipocyte differentiation in vitro and significantly reduced BAT-selective markers in vivo. In summary, this work provides a comprehensive human adipose catalog built from diverse fat and establishes a roadmap to facilitate the discovery of functional lncRNAs in adipocyte development. Read the article here: https://lnkd.in/dzss8JNv #metabolism

🔬 A recently published study by Nadjar et al. in Science Signaling introduces an optogenetic method to activate inflammasomes, inducing pyroptosis – a form of programmed cell death. Their tool allows precise manipulation of inflammasome activation, shedding light on cellular mechanisms. More on their method 👉 https://lnkd.in/deZ8fyV4 #Science #Inflammasomes #Pyroptosis 🧬🔦