Medical laboratories specialist | Molecular biology and forensic genetics | Membership in the Saudi Society for Molecular Biology and Biotech
Problems we face in #DNA_extraction and their reason: 🔴Little or no #DNA in the eluate? -reason- 1-Low concentration of cells in the sample. 2-Inefficient cell lysis insufficient mixing with Buffer AL. 3- Inefficient cell lysis due to decreased protease activity. 4-No ethanol added to the lysate before loading onto the QIAamp column. 5-Buffer AW1 or Buffer AW2 prepared incorrectly. 6-Water used instead of Buffer AE for elution. 🛑Low concentration of #DNA in the eluate? 1-Second elution step diluted the DNA