es/iode’s Post

📃Scientific paper: Localization of EccA_3 at the growing pole in Mycobacterium smegmatis Abstract: Background Bacteria require specialized secretion systems for the export of molecules into the extracellular space to modify their environment and scavenge for nutrients. The ESX-3 secretion system is required by mycobacteria for iron homeostasis. The ESX-3 operon encodes for one cytoplasmic component (EccA_3) and five membrane components (EccB3 – EccE3 and MycP_3). In this study we sought to identify the sub-cellular location of EccA_3 of the ESX-3 secretion system in mycobacteria. Results Fluorescently tagged EccA_3 localized to a single pole in the majority of Mycobacterium smegmatis cells and time-lapse fluorescent microscopy identified this pole as the growing pole. Deletion of ESX-3 did not prevent polar localization of fluorescently tagged EccA_3, suggesting that EccA_3 unipolar localization is independent of other ESX-3 components. Affinity purification - mass spectrometry was used to identify EccA_3 associated proteins which may contribute to the localization of EccA_3 at the growing pole. EccA_3 co-purified with fatty acid metabolism proteins (FAS, FadA3, KasA and KasB), mycolic acid synthesis proteins (UmaA, CmaA1), cell division proteins (FtsE and FtsZ), and cell shape and cell cycle proteins (MurS, CwsA and Wag31). Secretion system related proteins Ffh, SecA1, EccA1, and EspI were also identified. Conclusions Time-lapse microscopy demonstrated that EccA3 is located at the growing pole in M. smegmatis . The co-purification of EccA_3 with proteins known... Continued on ES/IODE ➡️ https://etcse.fr/Lvx ------- If you find this interesting, feel free to follow, comment and share. We need your help to enhance our visibility, so that our platform continues to serve you.

Exciting to share our latest publication which delves into the intricate world of Bacteroides bacterial extracellular vesicles (BEVs). 🧫 In this study, we investigated the temporal dynamics of BEV release throughout the growth cycle, uncovering distinct phases with varied vesicle compositions. Early in growth, we observed a prevalence of outer membrane vesicles enriched in lipoproteins, whereas late-phase BEVs exhibited lytic vesicles with cytoplasmic protein dominance. One key finding is the preferential incorporation of lipoproteins with negatively charged signal peptides into BEVs. This discovery opens avenues for predicting and understanding the functions of B. thetaiotaomicron BEV-enriched lipoproteins, providing valuable insights into the bacterial adaptive strategies. Our research emphasizes the critical role of media composition in BEV studies, advocating for the use of chemically defined media to ensure accurate characterization. Additionally, we address the challenge of vesicle heterogeneity and propose mechanisms for selective lipoprotein incorporation. This study not only contributes to the growing body of knowledge on BEVs but also unveils potential applications in understanding bacterial communication, adaptation, and long-term survival within the gastrointestinal tract. 📖 Read the full open access paper for a deep dive into the fascinating world of B. thetaiotaomicron BEVs and their implications for microbiology and gut health. #Microbiology #Research #BacterialVesicles #Science #LinkedInArticle 🧬🔍📚 https://lnkd.in/dh6xXpfB

Our recent research work on Rhynchostylis retusa (L.) "Exploring the influence of geographical distribution on the polyphenol composition, antioxidant, antimicrobial, and cytotoxic properties of Rhynchostylis retusa (L.) Blume roots! 🌿✨ Discover how location shapes the medicinal potential of this remarkable orchid species, unlocking insights for future therapeutic applications. #MedicinalPlants #Phytochemistry #Antioxidants #GeographicalImpact #Research"

The inner surfaces of the intestinal tract are lined with a mucus layer that forms the habitat for the microbiome. Mucus consists of mucins, large proteins that are densely glycosylated with GalNAc-type O-glycans. Many gut bacteria produce glycosidases and glycopeptidases, which are enzymes that cleave mucus glycans and the protein core. The sugars and amino acids that are released from mucins serve as nutrients for the bacteria. Enzymes involved in mucus degradation play therefore an important role in feeding, shaping, and maintaining the microbiome. Although glycopeptidases that cleave O-glycopeptides have been reported, enzymes that specifically recognize and cleave dense O-glycan clusters of mucins remained elusive.   A multidisciplinary research team comprising scientists from Denmark, Spain, France, and the Radboud Consortium for Glycoscience has now discovered a new family of mucinases that specifically cleaves mucin O-glycan clusters. They utilized a sequence modular walk strategy, cleavage assays based on recombinant human mucins, as well as crystallography with molecular dynamics (MD) simulations. Through these approaches, they identified mucinases that utilize two glutamic acid residues for catalytic cleavage of mucin O-glycan clusters. The mucinases were identified in prokaryotes (bacteria) and eukaryotes (like fungi and sea lice) suggesting biological roles for mucinases beyond the intestinal microbiome. The discovery of this new family of mucinases represents a significant advancement in understanding the intricate dynamics of the host-microbiome relationship.   Check out the full publication via: https://lnkd.in/e7m5cxiy

A transcriptome-based analysis reveals functional differences among Dendrobium officinale Kimura & Migo species from different growing regions and with different quality levels https://lnkd.in/gC4st_zr Dendrobium officinale Kimura & Migo (D. officinale) is a common, high medicinal value food and herbal medicine used in the treatment of yin-deficient diseases. Adulterants obtained with similar inexpensive species of Dendrobium plants are available in the market. However, Dendrobium species with different quality levels or from different regions as well as analogous species have a similar appearance, which makes the discrimination of Dendrobium species difficult. Reliable objective methods for authenticating D. officinale are lacking. Here, samples of Dendrobium officinale Kimura & Migo (D. officinale) from two regions with three quality levels and sample of the analogous species Dendrobium compactum Rolfe ex W. Hackett (D. compactum) were subjected to genome-wide biological response fingerprinting (BioReF) in RAW264.7 cells by RNA sequencing. The biological pathways affected by these samples could distinguish D. officinale from D. compactum. The high-quality D. officinale samples from Yunnan exhibited the strongest inhibition of lipopolysaccharide (LPS) induced inflammatory genes and pathways, followed by samples from Myanmar. The medium- and low-quality samples from Yunnan exhibited weaker effects, and D. compactum exerted mild effects. The RNA sequencing results were strengthened by the results from analyses of the expression of the inflammation-related genes Il6, Ccl5, and Cox2 and the production of nitric oxide. Thus, transcriptomic profiling can be used to evaluate the quality of D. officinale and distinguish its origin. #Dendrobium officinale Kimura & migo #Transcriptome analysis #RNA sequencing #Region #Quality levels #technology #research #innovation

Hello, connections! 👋 🤩 I am super excited to announce that my first scientific publication is now live on bioRxiv! The paper is titled "Dicarboxylic acids synergize with the yeast and human Hsp60/10 systems to mimic GroEL/ES." You can read it here:  (https://lnkd.in/gji7HxSJ) 📄 🤯 In this study, we show for the first time how small molecules can collaborate with "chaperonins" of eukaryotes (Hsp60/10) in vitro and alter its folding capacity, highlighting the potential of protein-folding regulation via chaperonins using small molecules. It lays foundation for the link of metabolism with the evolution and folding capability of chaperone machinery. 🤓 GroEL/ES is a well known class-I chaperonin from prokaryotes famous for its role in protein-folding and fascinating barrel-like structure. This nano-cage is also present in mitochondria and chloroplasts of eukaryotes and are essential for all the organisms. The exact mechanisms of substrate protein specificity is not well elaborated to this date. What is known recently is that these cute little nano-cages have differential cavity charges, across the species. GroEL/ES carries one of the most negative one. Human and yeast counterparts, on the other hand, have one of the least negative charges in their "folding chamber", and are unable to fold substrates of GroEL/ES in vitro. Intriguingly, adding negatively charged molecules, dicarboxylic acids in this case, changed the folding capacity of mitochondrial Hsp60/10s of yeast and human, enabling them to fold exactly like GroEL/ES does. 🧐 We speculate that the evolution of mitochondrial chaperonins could have been shaped by its cellular milieu. The metabolites in mitochondrial matrix can increase or decrease the folding capacity of the Hsp60/10s. This cooperation between small molecules and mitochondrial chaperone machinery could be harnessed by the cell to regulate proteostasis, through the regulation of metabolism. 🤔 💭 This research is particularly exciting as it opens up potential avenues for addressing protein folding diseases and enhancing our understanding of protein folding mechanism and mitochondrial proteostasis. Thank you for your interest. 🙏 #ProteinFolding #Proteostasis #MitochondrialProteostasis #ScientificResearch #bioRxiv #Chaperonins #Biophysics #Biochemistry #MolecularBiology #ProteinBiology

I am inspired by the expanding field of immunometabolism, which successfully targets the immune system to address metabolic diseases like diabetes. Therapies such as interleukin-1 receptor antagonists, TNFα inhibitors, and Glucagon-Like Peptide-1 offer promising potential to improve glycemic control, insulin sensitivity, and other metabolic functions. These advancements provide valuable insights into disease mechanisms and pave the way for innovative therapies for various metabolic and inflammatory conditions. I am genuinely excited and optimistic about the progress in research on inborn errors of metabolism (IEM). I hope for groundbreaking treatments that alleviate metabolic stress, strengthen immunity, and offer hope for better management and improved quality of life. #Immunometabolism #MetabolicHealth #Type2Diabetes #InbornErrorsOfMetabolism #IEM #GlycemicControl #InsulinSensitivity #Immunology #ResearchInnovation #MetabolicDisorders #HealthyLiving #HealthcareResearch #LifeSciences #ChronicDiseaseManagement #FutureOfMedicine

The abstract submission deadline for the 2024 IMGC Symposium co-hosted by UC Davis on October 22-24, 2024 has been extended one week to March 15, 2024! Oral presenters receive complimentary registration and travel reimbursed. Don't miss the opportunity to share your exciting research through the IMGC! Session topics include: -Current Discoveries in Comparative Biology & Lactation Physiology -Bioactive Lipids, Proteins & Peptides -Emerging Discoveries in Milk, Gut and Brain -Milk Research, Nutrition and Clinical Outcomes -Breakthrough Innovations in Milk Extracellular Vesicles & miRNA -Hottest Topics in Milk Science https://lnkd.in/g2n7THrM

📃Scientific paper: Proteome profiling of Campylobacter jejuni 81–176 at 37 °C and 42 °C by label-free mass spectrometry Abstract: Background The main natural reservoir for Campylobacter jejuni is the avian intestinal tract. There, C. jejuni multiplies optimally at 42 °C – the avian body temperature. After infecting humans through oral intake, the bacterium encounters the lower temperature of 37 °C in the human intestinal tract. Proteome profiling by label-free mass spectrometry (DIA-MS) was performed to examine the processes which enable C. jejuni 81–176 to thrive at 37 °C in comparison to 42 °C. In total, four states were compared with each other: incubation for 12 h at 37 °C, for 24 h at 37 °C, for 12 h at 42 °C and 24 h at 42 °C. Results It was shown that the proteomic changes not only according to the different incubation temperature but also to the length of the incubation period were evident when comparing 37 °C and 42 °C as well as 12 h and 24 h of incubation. Altogether, the expression of 957 proteins was quantifiable. 37.1 − 47.3% of the proteins analyzed showed significant differential regulation, with at least a 1.5-fold change in either direction (i.e. log_2 FC ≥ 0.585 or log_2 FC ≤ -0.585) and an FDR-adjusted p -value of less than 0.05. The significantly differentially expressed proteins could be arranged in 4 different clusters and 16 functional categories. Conclusions The C. jejuni proteome at 42 °C is better adapted to high replication rates than that at 37 °C, which was in particular indicated by the up-regulation of proteins belonging to the functional categories “replicatio... Continued on ES/IODE ➡️ https://etcse.fr/DIKa ------- If you find this interesting, feel free to follow, comment and share. We need your help to enhance our visibility, so that our platform continues to serve you.

📃Scientific paper: Sub-lethal concentration of a colloidal nanosilver formulation (Silversol®) triggers dysregulation of iron homeostasis and nitrogen metabolism in multidrug resistant Pseudomonas aeruginosa Abstract: Background Pseudomonas aeruginosa is a notorious pathogen. Its multidrug resistant strains are listed among priority pathogens against whom discovery of novel antibacterial agents and, elucidation of new anti-pathogenicity mechanisms are urgently warranted. This study describes multiple antibacterial effects of a colloidal nano-silver formulation- Silversol® against a multi-drug resistant strain of P. aeruginosa . Results Minimum inhibitory concentration (MIC) of Silversol® against P. aeruginosa was found to be 1.5 ppm; and at sub-MIC of 1 ppm, it was able to alter quorum-sensing regulated pigmentation (pyocanin 82%↓; pyoverdine 48%↑), exopolysaccharide synthesis (76%↑) and biofilm formation, susceptibility to antibiotics (streptomycin and augmentin), protein synthesis and export (65%↑), nitrogen metabolism (37%↑ nitrite accumulation), and siderophore production in this pathogen. Network analysis of the differentially expressed genes in the transcriptome of the silversol-treated bacterium identified ten genes as the potential molecular targets: norB, norD, nirS, nirF, nirM, nirQ, nosZ, nosY, narK1, and norE (all associated with nitrogen metabolism or denitrification). Three of them (norB, narK1, and norE) were also validated through RT-PCR. Conclusions Generation of nitrosative stress and disturbance of iron homeostasis were found to be the major mechanisms associated with anti- Pseudomonas activity of Silversol®. Continued on ES/IODE ➡️ https://etcse.fr/wKFXj ------- If you find this interesting, feel free to follow, comment and share. We need your help to enhance our visibility, so that our platform continues to serve you.