ESACT - European Society for Animal Cell Technology’s Post

Accelerate your assay development and pave your path to a more successful and sustainable future with Meridian’s Air-Dryable and Lyo-Ready qPCR and LAMP molecular master mixes! Room temperature shipping and storage are crucial in reducing a product’s carbon footprint. Explore features of Meridian’s Air-Dryable and Lyo-Ready molecular mixes: 1. Designed to create room-temperature stable assays that do not require refrigeration 2. Have an extended shelf-life of 1 to 2 years 3. Optimized for sensitive detection from extracted RNA/DNA or from crude clinical samples (blood, saliva urine or stool) Click here to request a sample to test: https://lnkd.in/gA2y5Wcf #moleculardiagnostics #pointofcaretesting #sustainability

The first step in designing targeted protein degraders is single-point screening for warheads and ligase ligands. Getting this step right can be what makes the difference in getting a degrader to clinical trials. So, how do you make sure you’re getting this step right? ✅ Answer: Use biophysical tools early in the screening process! They provide more robust data than biochemical techniques and give you insight into the structure-activity relationships of your molecules. 💡 Now, what if there was a biophysical tool that is as fast and easy to use as biochemical techniques? Spectral Shift technology fits the bill — with in-solution, mass-independent affinity measurements, you can quickly and easily identify the best degrader candidates. Watch Jan Schnatwinkel’s webinar to learn more: https://bit.ly/462MBxZ

🕰️Your time is valuable. Get answers and publish faster! Generating your own recombinant proteins in the lab is difficult. Low yield and purity are challenges. Let MVPro recombinant proteins from OriGene accelerate your research: https://bit.ly/3I8LRw6 #recombinantproteins

Quantitative interpretation of #WesternBlot data is often a challenge due to improper standardization. For researchers trying to perform comparative analysis of protein expression between biological samples that vary in complexity, disease state or applied external stimuli, an approach that reproducibly measures legitimate variance is especially important. The #SimpleWestern "#Jess" from #BioTechne gives you an easy way to see if your samples contain a consistent #protein load - just load the proprietary in-capillary protein normalization reagent into the assay plate and she’ll take care of the rest. Download the application note here: https://lnkd.in/eTZA9ZBP

Before UPLC columns were introduced in 2004, chromatographers were trying to decrease run times by using short columns for high thoughtput methods. However, larger molecules did not chromatograph well on the very short columns.  Hydrophilic Interaction Chromatography (HILIC) was adopted in the 2015 era as a useful tool for characterizing protein glycosylation, largely because of its ability to separate highly polar species. Thanks to pioneering work by Davy Guillarme’s research team, we have taken a fresh look at these short columns for #biotherapeutic analysis. Read more about 5 minute LC/MS separations of protein glycoforms here:   https://lnkd.in/ewcZPssm

The #SimpleWestern #Immunoassay from #BioTechne is the gel-free, #blot-free and hands-free solution for researchers looking for a better way to get their Western blot data. The simple fact that you get analyzed data in just three hours with only 30 minutes of hands-on time changes things forever! So, since we think change is a good thing, we packed all that Simple Western speed, simplicity and data #quality into our Total Protein Assay. #Quantitation to monitor the expression of proteins or the purification process is now not dependent on varying house keeping proteins but on the total protein of your sample. See how you get more #precision and real quantitation in your immunoassay. Download the application note here: https://lnkd.in/dr2Ax5dt

🌈 Dive into the vibrant world of microbiology with our latest blog post! 🧫✨ Explore "The Colorful History of Chromogenic Culture Media," shining a spotlight on our very own HardyCHROM™ Chromogenic Agar. 🎨🔬 Uncover the fascinating journey of chromogenic media and its impact on microbial identification. Read more on our blog now! 👉 https://bit.ly/3wqXhIN #ChromogenicMedia #MicrobiologyHistory #HardyDiagnostics #HardyCHROM #ScienceInColor

Available CRISPR tools can seldom achieve the efficiency and precision to eliminate off-target effects and enable the highest level of precision, such as allele-specific editing Optimization is the second essential step of our approach to matching nucleases to diseases. Requiring a unique combination of protein engineering, guide design, and library selection, optimization helps our nucleases achieve even allele specificity while maintaining high activity with non-detectable off-target effects. Learn more: https://bit.ly/3KKLjxR #GeneEditing #PrecisionMedicine #EmendoBio

They write: The affinity and selectivity of small molecules for proteins drive drug discovery and development. We report a fluorescent probe cellular binding assay (FPCBA) for determination of these values for native (untagged) proteins overexpressed in living cells. This method uses fluorophores such as Pacific Blue (PB) linked to cell-permeable protein ligands to generate probes that rapidly and reversibly equilibrate with intracellular targets, as established by kinetic assays of cellular uptake and efflux.