Eurofins Viracor’s Post

#MagGenome #RNaseA is an #endoribonuclease that targets single-stranded RNA, cleaving phosphodiester bonds between the 5'-ribose of one nucleotide and the phosphate group of the 3'-ribose of an adjacent pyrimidine nucleotide. It yields a 2',3'-cyclic phosphate, which is then hydrolyzed into the corresponding 3'-nucleoside phosphate. It has a specific activity of 23,000 U/mg protein. Applications: - #Plasmid and #genomic #DNA preparation - RNA removal from #recombinantprotein preparations - Ribonuclease protection assays - Mapping single-base mutations in DNA or RNA MagGenome's Molecular Biology Grade Range Includes #DNAPolymerases (#Taqdnapolymerase/#PFUdnapolymerase) #PCRMastermixes #dNTPs #DNAladders #Agarose #Rnase #loadingDyes, #Buffers and Other Solutions

The core of the NGS workflow requires adding enzymes and buffers to nucleic acid samples. In the simplest case of DNA sequencing experiments, the I.DOT can be used to dispense fragmentation enzyme/buffer, ligation enzyme/buffer and amplification mastermix. Read more about NGS library preparation here: https://lnkd.in/dXAduFaN #IDOT #Technology #Automation #LifeSciences #LiquidHandling #LabAutomation #Development #DrugDiscovery #Genomics #SyntheticBiology #LaboratoryAutomation #FutureOfHealth

One of the most difficult elements of microbiology is identifying "molds"and 'multicellular fungi'. Additionally, the proliferation of different cell types in fungal cultures can influence mass spectra, making state-of-the-art 'MALDI-TOF' techniques susceptible to these effects. We offer a powerful answer in the form of a dedicated software module that initiates mass spectral capture with weighting parameters that differ from those typically used for bacteria. A high identification success rate is attributable to the efficient collection and analysis of data. For more information please visit https://lnkd.in/dkTD2q4X #Bioinnovations #MALDITOFMS #BIOTYPER #Fungiidentification #moldes #massspectrometry #database

The core of the NGS workflow requires adding enzymes and buffers to nucleic acid samples. In the simplest case of DNA sequencing experiments, the I.DOT can be used to dispense fragmentation enzyme/buffer, ligation enzyme/buffer and amplification mastermix. Read more about NGS library preparation here: https://lnkd.in/dXAduFaN #IDOT #Technology #Automation #LifeSciences #LiquidHandling #LabAutomation #Development #DrugDiscovery #Genomics #SyntheticBiology #LaboratoryAutomation #FutureOfHealth

NanoRanger is a novel approach that enables rapid and accurate mapping of complex genomic breakpoints with minimal sample requirements and low cost. Fowzan Alkuraya, Mo Li and colleagues describe a cohort of 16 familial cases, each harboring homozygous rearrangements that defied breakpoint determination by conventional techniques. NanoRanger identified the breakpoints with single-base-pair resolution, enabling accurate determination of the carrier status of unaffected family members as well as the founder nature of these genomic lesions and their frequency in the local population. #InnovationInGenomics #PrecisionMedicine #GenomicBreakpoints #NanoRanger #GeneticTesting #CarrierScreening #PatientCare https://lnkd.in/g87nfFSB

The glandular expression pattern of salivary gland-specific protein CA6 is highlighted by multiplex immunohistochemistry Upcoming additions to The Human Protein Atlas will include mIHC/IF-based exploration of protein expression in additional tissues, including salivary gland. CA6 - a carbonic anhydrase with an unclear role in salivary gland and excreted saliva - shows localization (green) in the cytoplasm of serous acini (teal). Other visualized cell types/structures include mucus acini (white) and large ducts (red). The spatial expression data is further supported by the single-cell RNA sequencing data for CA6 available in the Single Cell Type section showing enriched expression in serous glandular cells of the salivary gland. https://lnkd.in/dy_Y8P8t

Having your analytical workflow in optimal shape means nothing if your pre-analytics are not at the same level. Join the new scheme from EMQN CIC and SensID GmbH and learn how you are doing. #cfDNA #plasma #extraction

#CDK-independent role of D-type cyclins in regulating DNA mismatch repair:- •Although mismatch repair (#MMR) is essential for correcting DNA replication errors, it can also recognize other lesions, such as oxidized bases. In G0 and G1, MMR is kept in check through unknown mechanisms as it is error-prone during these cell cycle phases. •We show that in mammalian cells, D-type cyclins are recruited to sites of oxidative DNA damage in a PCNA- and p21-dependent manner. •D-type cyclins inhibit the proteasomal degradation of p21, which competes with MMR proteins for binding to PCNA, thereby inhibiting MMR. •The ability of D-type cyclins to limit MMR is CDK4- and CDK6-independent and is conserved in G0 and G1. •At the G1/S transition, the timely, cullin-RING ubiquitin ligase (CRL)-dependent degradation of D-type cyclins and p21 enables MMR activity to efficiently repair DNA replication errors. •Persistent expression of D-type cyclins during S-phase inhibits the binding of MMR proteins to PCNA, increases the mutational burden, and promotes microsatellite instability. #highlights :- •D-type cyclins are recruited to oxidative DNA lesions to inhibit MMR activity. •This role is present in G0 and G1 and is independent of CDK4 and CDK6 activity. •D-type cyclins’ degradation at G1/S ensures proper MMR activity during S-phase. •Absence of D-type cyclins in S limits mutational burden and microsatellite instability. #genetics #cellcycle #dnarepair #CDK4 #genomestability #molecularbiology

🧬 As many research areas move toward multiomic approaches, the AVITI24™ system re-imagines what is possible with a single platform. ⚡ 📹 Watch a recent presentation from Shawn Levy, PhD, CSO & SVP Applications, to learn how the #AVITI24 empowers analysis of DNA, RNA, proteins, phosphorylated, proteins, and cell paint from a single sample in under 24 hours. 🌟 Discover how the AVITI24 provides comprehensive multiomic, multidimensional readouts for an unprecedented view of cell morphology and function: https://bit.ly/3zUVdL2 #AVITI24 #ElemBio #Genetics #Genomics #Sequencing #Singlecell #DNASequencing #Multiomic

#Citation Spotlight 🌟 Transfectamine™ 5000 Transfection Reagent is a powerful and versatile transfection reagent for the introduction of nucleic acids into eukaryotic cells, or more specifically, into animal cells. It can be used for plasmid DNA transfection as well as siRNA- and shRNA-based gene knockdown experiments and gene expression studies. The low toxicity of Transfectamine™ 5000 also allowed higher viability of transfected cells. Once again, Transfectamine™ 5000 was validated by the scientific research experiment. 💯 Read the #fulltext paper Published in May 2024 via elifesciences https://lnkd.in/gW8Shprr #gene #DNA #research #biotech