Our blog post summarizes how polarized cell monolayers are the gold standard for studying P-gp and BCRP interactions as indicated in regulatory guidances and as such should be the first choice of test system for identifying P-gp or BCRP substrates and inhibitors. However, if results indicate the compound has inherently low passive permeability then P-gp or BCRP expressing membrane vesicles should be considered as an alternative follow up in vitro test system to mitigate against the clinical implications of false negatives. Read the blog on the Evotec Science Pool here - https://hubs.ly/Q02bYRML0 #researchneverstops
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Our blog post summarizes how polarized cell monolayers are the gold standard for studying P-gp and BCRP interactions as indicated in regulatory guidances and as such should be the first choice of test system for identifying P-gp or BCRP substrates and inhibitors. However, if results indicate the compound has inherently low passive permeability then P-gp or BCRP expressing membrane vesicles should be considered as an alternative follow up in vitro test system to mitigate against the clinical implications of false negatives. Read the blog on the Evotec Science Pool here - https://hubs.ly/Q02bYRt20 #researchneverstops
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💡 Did you know that the majority of #GPCR structures have been solved with cryo-EM? 🔬 GPCRs have an inherent complexity and often destabilize outside of their native cell membrane environment, making them difficult to study with traditional methods. #cryoEM overcomes these challenges. 👩🔬 Join Dr. Emily Armbruster, CCO Kristin Rider, and Hannagh Smith at Hanson Wade Group's GPCRs-Targeted Drug Discovery Summit next week, March 6-7 in Boston to learn how you can get a structure in under 4 weeks. 📖 Learn more about high resolution data collection for GPCRs: https://lnkd.in/gvwEgvET
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Sybodies(Synthetic nanobodies)…
At today's Cambridge Ion Channel Forum, Kathryn Smith, demonstrates how llama-derived nanobodies can be identified for the functional modulation of TREK2 K2P K+ channels and how they can be engineered to improve their affinity and efficacy. She also shows how the selection of synthetic nanobodies (Sybodies) can be biased towards the identification of selective activators. Using a combination of structural, biophysical and electrophysiological methods we show how these can provide insight into the mechanism of K2P channel structure and function. #ionchannels #cicf24 #drugdiscovery #conference #pharma #lifesciences
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'Building' up to the Holiday Season - Day 16. Surface Plasmon Resonance (SPR) is a real-time technique for studying molecular interactions and is commonly used in drug discovery to analyse binding kinetics and affinity on a metal surface. At Peak Proteins we produce high quality protein, tailor-made for these studies. As part of Sygnature Discovery we use SPR for hit identification, orthogonal hit confirmation and hit-to-lead activities. ➡️ Learn about out SPR capabilities - https://lnkd.in/eTFQMDhU This model depicts antibody binding to an SPR chip. 🎄🚪1️⃣6️⃣
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MEDY enables the #cryopreservation of cortical organoids without compromising their structure or function. Is this a success? This new technique (methylcellulose, ethylene glycol, DMSO, and Y27632) preserves crucial features, maintaining synaptic function and preventing harmful cellular processes. Its adoption is poised to transform research, medical diagnostics, and drug development by offering a cost-effective way to store these vital resources for future studies. Our CEO Dr. Emil Kendziorra "It is noteworthy to observe the growing number of research groups engaging in neuropreservation and the expanding interest in this field; this milestone serves as a testament to the increasing recognition of its importance. We can confidently view this as further validation of the expertise in the field." 🔗 Find the paper here: https://ow.ly/OI5g50RTNwm 📷 Survival of progenitor cells and neurons in day 90 cortical organoids after MEDY cryopreservation through Sox2 and MAP2 immunofluorescence staining. Scale bars, 100 μm.
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Elevate your research on JAK-STAT signaling with Revvity's AlphaLISA SureFire Ultra Assays, tailored for targets like JAK1/2, STATs, and SMAD proteins. Dive deep into the signaling cascades that drive critical cellular processes, from growth and development to immune response. With our AlphaLISA SureFire Ultra assays, you gain the precision, sensitivity, and reliability necessary to dissect the complexities of JAK-STAT signaling, paving the way for therapeutic breakthroughs and innovative research discoveries. Join us in advancing the frontier of cellular signaling research! http://ms.spr.ly/6042YkgTe #JAKSTATSignaling #CellularResearch #AlphaLISASureFireUltra #Revvupresearch
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📔[BROCHURE] 𝐌𝐨𝐧𝐢𝐭𝐨𝐫 𝐁𝐢𝐨𝐥𝐨𝐠𝐢𝐜𝐚𝐥 𝐂𝐡𝐚𝐧𝐠𝐞𝐬 𝐢𝐧 3𝐃 𝐂𝐞𝐥𝐥 𝐂𝐮𝐥𝐭𝐮𝐫𝐞𝐬 🧫 Three-dimensional (3D) cell cultures are widely employed from basic research to drug discovery/development because of their physiological relevance. The use of assays originally designed for cell monolayers (2D) or suspensions, however, may yield inaccurate or misleading results. ✅ Many Promega assays have already been validated to show good performance and produce accurate results with different 3D cell culture models. 👉 Explore more: https://bit.ly/4aIhOYE
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Study highlights the potential of an ML tool to predict the host receptor targeted by the receptor binding protein of 3 phages that target a carbapenem-resistant clinical isolate of E. coli U1007, and proves the utility of phage cocktail to restrict E. coli U1007 in vivo. https://lnkd.in/ew4JmpuY
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As part of an accelerating drive to identify model systems that more accurately predict human physiological responses to drug interventions, senior principal scientist at Visikol Inc. Dr. Amir Wahba, addresses the following topics in his webinar: • The challenge of drug-induced hepatoxicity • Development of predictive in vitro model systems • The combination of Visikol’s HUREL and Agilent’s xCELLigence Technology Watch now: https://bit.ly/49O2Lvf
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