Gennova Biopharmaceuticals’ Post

This week's selection of articles and #preprints on #proteostasis thanks to @Bims_BiomedNews -- https://lnkd.in/eXwT2M_i Highlight: RNA and protein degradation to counteract transcriptional burden and proteotoxic stress in human aneuploid cells https://lnkd.in/eaTcSEAs

rRRR removes rRNAs from 10 ng to 20 µg of input RNA extracted from different sources using an RNase H approach. rRRR is cost-effective and the rRNAs-depleted RNA can be used for various applications. https://bit.ly/4dWXzs8

The RNA ligase (LIG) that catalyzes the splicing of fungal tRNA exons requires a 2'-phosphate to form a splice junction. The three steps of the fungal LIG reaction pathway are shown.  https://bit.ly/4d84YDH

🧬 RNA: Single and Available ☝ 🔬 Ribonucleic acid (RNA) is a form of nucleic acid which is present in all living cells and has structural similarities to DNA. 💡 🤔 Often depicted as single-stranded molecule, RNA has a backbone made of alternating phosphate groups and the sugar ribose. RNA is available in different forms such as messenger RNA (mRNA), ribosomal RNA (rRNA) and transfer RNA (tRNA). 🤓 🧪🧫 Find out you can store and stabilize RNA samples, disrupt and homogenize samples to the highest RNA yield, perform common RNA calculations, and use advanced technologies for RNA sample QC: https://lnkd.in/gZmrs6za #FridayFacts #QIAGEN #RNA

Circular RNA (#circRNA) molecules are a novel type of #ncRNA in the form of a covalently closed loop lacking 5’-end caps and 3’-poly(A) tails. The stable structure endows the circular transcripts with resistance to exonuclease digestion. Our circRNA sequencing service (circRNA-seq) supports the investigation of these transcripts, which act as #miRNA sponges in their regulatory function in #geneexpression. https://lnkd.in/eaYdnTSj #nextgenerationsequencing

Back to Basic: Determination of the numbers of reticulocytes or immature, non-nucleated RBCs that still contain RNA provides useful information about the capacity of the bone marrow to synthesize and release red cells in response to anemia and helps to distinguish between decreased RBC production and enhanced peripheral destruction. Corrected reticulocyte counts or the reticulocyte production index (RPI) can be used to compare the magnitude of reticulocytosis with the magnitude of anemia to determine whether the bone marrow response is adequate. #Ahmed_MLT_Sudan

Mango Beacons, based on the RNA Mango aptamer, fluoresce upon binding to a trigger RNA sequence. Multiple strategies for forming high contrast Mango Beacons are presented. bit.ly/3SRheRS

Have you ever spent hours on RNA extraction only to end up with very small yield? Or perhaps you have spent the whole day working on your experiment only to find out at the end of the PCR step that contamination ruined your results? Don't worry, you are not alone on this. To make your life a little easier for the next time, we have decided to give you some background on why RNA degradation can happen in the lab and tips on how to save your RNA. For example, did you know that the air conditioner can be a source of contamination? Read more from our blog 👉 https://lnkd.in/eTj4THq7 #RNA #Educational #Biotech #Tips

The HCV genome is terminated by a conserved untranslated RNA known as 3’X. The first 55-nt of 3’X adopt two distinct conformations that may regulate different aspects of viral biomolecular synthesis. https://bit.ly/4cvkWbe

Deliver CRISPR wise Small extracellular vesicles (sEVs) the modular safeEXO platform, a prototype for delivering small extracellular vesicles (sEVs) that is largely free of endogenous RNA. This platform can effectively deliver RNA and ribonucleoprotein (RNP) complexes to their intended intracellular targets, resulting in observable downstream biological activity https://lnkd.in/gNemqujA