HDT Bio’s Post

: The pursuit of longevity has been a longstanding goal of humanity, and advances in medical science have brought us closer to achieving this aspiration. Recently, RNA vaccines have emerged as a revolutionary tool in the field of vaccinology, demonstrating remarkable efficacy against infectious diseases. Building on this success, there is growing interest in exploring the potential of RNA vaccines for applications beyond infectious disease control, including longevity enhancement. This research proposal aims to investigate the feasibility and efficacy of RNA vaccines in promoting longevity.

Study by researchers at University of Texas Medical Branch (Galveston) reveals the NSP1 and NSP15 SARS-CoV-2 proteins as major players in antagonizing the innate immune response against the virus. The authors generated recombinant viruses with mutations in either NSP1, NSP2, NSP3, NSP6, NSP12, NSP13, NSP14, NSP15, NSP16, ORF3a, ORF6 or ORF8, and tested their effect in several systems NSP1 and NSP15 increased the type I interferon response relative to the parental wild-type virus using an interferon-induced reporter cell line. Mutation of these genes in SARS-CoV-2 also led to increased expression of multiple genes involved in innate immune response, cytokine-mediated signaling and regulation of lymphocyte proliferation. Lastly, infection with SARS-CoV-2 lacking NSP1 or NSP15 mutants resulted in attenuated pathogenesis and lower replication of the virus in the respiratory tract of transgenic mice. #covid19 #Immunity #health #globalhealth #publichealth #medicine #biotechnology #pharmaceuticals #immunology

The pursuit of longevity has been a longstanding goal of humanity, and advances in medical science have brought us closer to achieving this aspiration. Recently, RNA vaccines have emerged as a revolutionary tool in the field of vaccinology, demonstrating remarkable efficacy against infectious diseases. Building on this success, there is growing interest in exploring the potential of RNA vaccines for applications beyond infectious disease control, including longevity enhancement. This research proposal aims to investigate the feasibility and efficacy of RNA vaccines in promoting longevity.

39% of those who were immunocompromised had evidence of changes in the gene that encodes the SARS-CoV-2 spike protein, compared with 12% of the non-immunocompromised group. In addition, clearance rates of nasal viral RNA and culturable SARS-CoV-2 differed among the immunocompromised groups. Nasal viral RNA can include SARS-CoV-2 fragments that aren’t infectious, but viral RNA that can be grown in culture is infectious. On average, this was how long it took the different groups to clear viral RNA and culturable virus from their nose: • For the group that included solid organ transplant recipients, 72 days for viral RNA, 40 days for culturable virus. • For the other severely immunocompromised group, 10 days for nasal viral RNA, 6.5 days for culturable virus. • For the less severely immunocompromised group, 12 days for nasal viral RNA, 6 days for culturable virus. • For the non-immunocompromised group, 13 days for nasal viral RNA, 7 days for culturable virus.

This is one of the most exciting posts on LinkedIn this year for me. The era of proper viral hygiene will begin with ARPA-H, just as the internet began with #DARPA (under its pre-DARPA name of just ARPA). #virus #viraldiagnostics #vaccine #vaccines #therapy #therapeutics #microbe #microbialdiagnostics #metagenomics

Recent advancements in mRNA-encoded antibody technology offer a potential solution to combat orthopoxviruses such as variola virus (VARV) and monkeypox virus (MPXV). 🦠 Using a lipid nanoparticle (LNP)-encapsulated mRNA platform, researchers engineered monoclonal antibodies targeting orthopoxviruses, demonstrating rapid production of neutralizing antibodies upon intravenous administration in mice. Notably, a unique mRNA antibody cocktail, Mix2a, showed superior in vivo protection by targeting both intracellular mature virus (IMV)-form and extracellular enveloped virus (EEV)-form viruses. These findings not only addresses the urgent need for effective therapeutic strategies against orthopoxviruses but also highlights the potential of mRNA antibody technology in combating other emerging infectious diseases. Read the paper here: https://lnkd.in/ekd8_bCt #Biointron #Antibodies #Immunotherapy #DrugDevelopment #DrugDiscovery #Variola #Virus #Healthcare #AntibodyProduction

This study presents an innovative approach to designing a multi-epitope vaccine against various SARS-CoV-2 variants using immunoinformatics and the fynomer scaffold. 🧬💉 By leveraging peptides from key structural proteins and optimizing the vaccine structure, the researchers conducted comprehensive bioinformatic analyses to ensure safety, efficacy, and broad population coverage. Molecular dynamics simulations and immune stimulation further validated the vaccine's potential, while codon optimization confirmed efficient expression. This fynomer-based vaccine represents a promising strategy for combating COVID-19 and future coronavirus diseases. #COVID19Vaccine #Immunoinformatics #VaccineDesign https://lnkd.in/dgmcJiFv

Happy Wednesday all! Check out this open access Cell Immunity review by Jason G. Cyster and Patrick C. Wilson, "Antibody modulation of B cell responses—Incorporating positive and negative feedback." Summary: Antibodies are powerful modulators of ongoing and future B cell responses. While the concept of antibody feedback has been appreciated for over a century, the topic has seen a surge in interest due to the evidence that the broadening of antibody responses to SARS-CoV-2 after a third mRNA vaccination is a consequence of antibody feedback. Moreover, the discovery that slow antigen delivery can lead to more robust humoral immunity has put a spotlight on the capacity for early antibodies to augment B cell responses. Here, we review the mechanisms whereby antibody feedback shapes B cell responses, integrating findings in humans and in mouse models. We consider the major influence of epitope masking and the diverse actions of complement and Fc receptors and provide a framework for conceptualizing the ways antigen-specific antibodies may influence B cell responses to any form of antigen, in conditions as diverse as infectious disease, autoimmunity, and cancer. #drugdiscovery #cancerresearch #antibodies #infectiousdisease #sarscov2 #autoimmunity #immunooncology #immunotherapy #scientificresearch

#immunology #cells #review  #medicine #medicalsciences https://lnkd.in/gW8nrMhr Abstract #Immune #memory — comprising T cells, B cells and plasma cells and their secreted antibodies — is crucial for human survival. It enables the rapid and effective clearance of a pathogen after re-exposure, to minimize damage to the host. When antigen-experienced, memory T cells become activated, they proliferate and produce effector molecules at faster rates and in greater magnitudes than antigen-inexperienced, naive cells. Similarly, memory B cells become activated and differentiate into antibody-secreting cells more rapidly than naive B cells, and they undergo processes that increase their affinity for antigen. The ability of T cells and B cells to form memory cells after antigen exposure is the rationale behind vaccination. Understanding immune memory not only is crucial for the design of more-efficacious vaccines but also has important implications for immunotherapies in infectious disease and cancer. This ‘guide to’ article provides an overview of the current understanding of the phenotype, function, location, and pathways for the generation, maintenance and protective capacity of memory T cells and memory B cells.

📣 New online paper annoucement 📣 📃 Title: Immunopathogenesis of Orthopoxviridae: insights into immunology from smallpox to monkeypox (mpox) ✒ Authors: Brent B., Ingo Fricke, Chinua Imarogbe,  Dr. Alexander Ariel Padrón González, Osvaldo Aguilera Batista, Pascal MENSAH, Enrique Chacon-Cruz 🎯 Antiviral therapeutic drugs were typically prescribed for smallpox and mpox disease; however, the molecular and immunological mechanisms with cellular changes remain of interest. Furthermore, no official authorized treatment exists for mpox disease. Some humans across the globe may be considered at risk. Historically, presenting symptoms of mpox resemble other viral diseases. Symptoms include rashes or lesions like Streptococcus, but also human herpes viruses (HHVs), including Varicella zoster virus (VZV). #newresearch #Adaptive #innate #immunology #orthopoxvirus #cellular #monkeypox #smallpox 💡 Join us and welcome to read this paper at 👇