Humanized Mice Laboratory’s Post

In this week’s #MAbMonday spotlight, we’d like to recognize the work on An Optimized Single-Molecule Pull-Down Assay for Quantification of Protein Phosphorylation. https://hubs.la/Q02bQzpX0 The team stated, "Phosphorylation is a necessary posttranslational modification that regulates protein function and directs cell signaling outcomes. Current methods to measure protein phosphorylation cannot preserve the heterogeneity in phosphorylation across individual proteins. The single-molecule pull-down (SiMPull) assay was developed to investigate the composition of macromolecular complexes via immunoprecipitation of proteins on a glass coverslip followed by single-molecule imaging." 👇Featured product👇 Anti-Human EGFR (External Domain) – Biotin: https://hubs.la/Q02bR3Mj0 #Leinco #ReproducibleScience #Immunology

Exploring how extracellular vesicle-depleted serum affects endothelial cell traits across time: Luiz Fernando Garcia, Pryscilla Wowk and Letusa Albrecht at FIOCRUZ - Fundação Oswaldo Cruz presented the first study comparing EV characteristics from human endothelial cells collected at two different culture times https://lnkd.in/gdJGp6Df Their findings revealed that human brain microvascular endothelial cells cultured with EV-depleted serum produce extracellular vesicles with evolving physical properties and varying protein levels over time #extracellularvesicles #exosomes #cellculture #proteomics #Vesiculab

G protein-coupled receptors are fascinating cell surface proteins that play crucial roles in a variety of cellular responses. Have a read if you want to learn more about TG2-induced GPR56 signaling - this paper contains part of the work I did during my PhD. Bauer, L.; Edwards, J.; Heil, A.; Dewitt, S.; Biebermann, H.; Aeschlimann, D.; Knäuper, V. Mesenchymal Transglutaminase 2 Activates Epithelial ADAM17: Link to G-Protein-Coupled Receptor 56 (ADGRG1) Signalling. Int. J. Mol. Sci. 2024, 25, 2329. https://lnkd.in/eiPKUDqP

#snsinstitutions #snsdesignthinkers #designthinking !!hey connection reorganization that promotes receptor dimerization, leading to the activation of the intracellular tyrosine kinase domain. How- ever, conclusions about the mechanism of ligand-induced dy- namic recruitment and dimerization of membrane-associated re- ceptors remains controversial Kusumi et al. (2012). Conven- tional steady-state ensemble approaches cannot be used to ad- dress the stochastic nature of activated receptors that encounter each other in a highly heterogeneous and Aluidic plasma mem- brane. Recent advancements in single-molecule lorescent "aging and tracking have provided further insights into the be- havior of EGFR in vivo Teramura et al. (2006); Chung et al. (2010); Hiroshimaa et al. (2012). For example, Lidke et al.de vised a two-color quantum-dot tracking method to visualize the state-dependent dimerization processes of human EGFR in liv- ing cells Low-Nam et al. (2011).A hidden Markov model was used 10 extract the kinetic paramcters of the underlying free., co- confined, and dimerized states. Researchers have increasingly determined that lipid domains rich in glycosphingolipids and cholesterol can facilitate signal- ing receptors to form a dimer Low-Nam et al. (201 1); Hess ct al. (2007); Lingwood and Simons (2010). Because the recruitment mechanism and dynamic clustering of receptors in the hierarchi- cal structure of plasma membranes are not clearly understood the existence and functionality of lipid nanoscale domains on EGFR dimerization remains controversial. A growing body of research indicates that membrane cholesterol can influence the organization, stability. and function of membrane proteins in- cluding receptors Orr et al. (2005); Ringerike et al. (2002): Pike and Cascy (2002). The process by which this cholesterol modu- lates membrane proteins remains unclear

Dive into the world of Cell Culture Media #Metabolomics with Panome Bio! Next-Generation Metabolomics from Panome Bio allows researchers to enhance process monitoring, optimize yields, and ensure product quality. To learn more, view a sample data report from one of our recent experiments, "Temporal Analysis of Metabolite Consumption and Production in Cell Culture Media." Download the data report here: https://hubs.ly/Q02bSjP90

Spatiotemporal Control of Inflammatory Lytic Cell Death Through Optogenetic Induction of RIPK3 Oligomerization Science Direct https://lnkd.in/gG3PJJPC

This comprehensive review focuses on the bioactive flavonoid #baicalein, extracted from Scutellaria baicalensis and Oroxylum indicum roots, extensively analyzing its pharmacological effects through in vitro and in vivo studies. Baicalein demonstrates diverse benefits, such as #anti-cancer, #antidiabetic, #antimicrobial, and #neuroprotective properties. The review emphasizes its potential as a complementary medicine, advocating for further clinical studies to validate its effectiveness and safety. | https://ow.ly/mRUL50Sf9iP

Our CEO, Shehnaaz Suliman, M.D. (MB ChB), M.B.A., M.Phil., discussed on Locust Walk how we prioritized research for our Selective Organ Targeting (SORT) Lipid Nanoparticle (LNP) platform: “We followed the science. We started in the lungs because no one else had gone there. And also because it’s about as far away […] from the liver as you can go. Showing proof of concept there with a novel formulation that is clearly not the liver de-risks not just the program and the modality, but the overall platform as well.” Utilizing our SORT LNP platform, we are working on therapies to reach all patients, including those for whom existing options are insufficient. Learn more about the next generation of genetic medicine delivery via the link below: https://lnkd.in/gjWyq3MZ

Synthetic Thiol Compounds Transform Endothelial Cell Dynamics  by Michela Bruschi, Awarded Best Short Oral Presentation at Redox Medicine 2024 🏆 ! Dr. Bruschi studied how synthetic thiol compounds affect endothelial cell redox modulation, metabolic profiles, and stress responses in varying oxygen levels. This research shows their potential to regulate cellular redox states, offering new therapeutic approaches for endothelial dysfunction. In her work at the University of Urbino, she focuses on fine-tuning cellular redox states with synthetic thiol compounds to address oxidative challenges like hypoxia or inflammation. Her findings could pave the way for future therapies in disease and aging. 🔗 Learn more about the award winning talks: https://lnkd.in/dZ452-XT 🔁 Missed the conference? Watch the replay and follow up with the award winning talks. 🌐: https://lnkd.in/dsCEQThq #RedoxMedicineSociety #RedoxMedicine2024 #redox #rms #thiol #syntheticthiol #oxidiativestress #AwardWinner #EndothelialFunction

I'm thrilled to announce the publication of my latest research in the Journal of Cell Science: "KRAP regulates mitochondrial Ca2+ uptake by licensing IP3 receptor activity and stabilizing ER–mitochondrial junctions". Understanding how intracellular calcium (Ca2+) dynamics are regulated is crucial for insights into cellular function and health. Our study sheds light on the role of Kras-induced actin-interacting protein (KRAP) in regulating Ca2+ transfer from the endoplasmic reticulum (ER) to mitochondria, a process essential for cellular energy production and signaling. This research provides new insights into the molecular mechanisms regulating intracellular Ca2+ dynamics, which could have implications for understanding and treating various diseases, including neurodegenerative disorders and cancers. I want to extend my heartfelt gratitude to my co-authors, collaborators and other incredible scientists I worked alongside, for their invaluable contributions to this project. A special mention to Adelina Ivanova, who is a phenomenal scientist and a joy to work with. Full paper available here --> https://lnkd.in/eRBExiPc #CellBiology #Mitochondria #ScientificResearch #Publication