IRBM’s Post

Unlocking the Mysteries of Protein Folding with Advanced Microscopy This insightful article sheds light on the pivotal role of advanced microscopy techniques in unraveling the intricate processes of protein folding. The experimental setup, showcased in a grid diagram, provides a glimpse into the sophisticated tools utilized to capture the dynamics of single molecules, such as bacteriorhodopsin (bR). Through the ingenious combination of atomic force microscopy and light triggers, researchers have gained unprecedented insights into membrane protein dynamics, paving the way for novel methodologies in drug development. https://lnkd.in/e6DC2NX8 As molecular components play a crucial role in drug development, understanding the mechanisms behind protein folding is paramount. With up to 50% of drugs targeting proteins, the study of model proteins like bacteriorhodopsin (bR) holds immense significance. As a leading microscopy company, Phaos is excited to see the transformative impact of advanced microscopy in unraveling the mysteries of molecular biology. Join us as we continue to champion the advancement of microscopy technology, empowering researchers worldwide to unlock new frontiers in science and medicine. #Microscopy #ProteinFolding #MolecularDynamics #DrugDevelopment #ScientificResearch #PhaosTechnology #Innovation

If you're at #AUC2024, don't miss the workshops Alexander Yarawsky and Michael DeLion are leading! Come learn more about BASIS - our cGMP SEDFIT program, how to use SEDANAL to analyze interacting systems, and the basics of studying interacting systems by AUC. Reach out if you're around and let us set up time to discuss more. https://lnkd.in/dGMrs9si #aav #genetherapy #rarediseases #biophysics #STEM #STEMPhilly #biochemistry #celltherapy #bioanalysisllc #PhillyBusiness #phillyscience

New Method to Investigate The Structure and Dynamics of Challenging Membrane Proteins #NMR spectroscopy is a versatile method for the investigation of the structure, dynamics and interactions of (bio-)molecules, such as proteins, at high resolution. 💡 A new approach, created by Prof. Franz Hagn and team markedly reduces the complexity of the NMR measurements and heavily facilitates the investigation of large and challenging membrane protein systems of pharmaceutical importance. “With this work, we achieve a breakthrough for future NMR experiments of membrane proteins that always suffered from their high complexity. It can be envisioned that only the functionally relevant part of a membrane protein can be visualized to simplify the experiments even more. Such an approach will speed up the analysis of membrane protein structures and their interaction with drug molecules by NMR.” Prof. Franz Hagn, Institute of Structural Biology, #HelmholtzMunich 👉 Learn more in our news:  https://lnkd.in/dUfqSg3Z #proteins #HighResolution #protein #research #MedicalApproach Technische Universität München #MedicalResearch #DrugDiscovery

I’m excited to share our recent paper, where we introduce a smart localized shimset integrated with an RF microsolenoid, optimized as a single unit to achieve high-resolution NMR spectroscopy. This innovation marks a significant step toward parallel NMR spectroscopy, demonstrating the ability to improve NMR line width from 84 to 4 Hz in a 1.05 T preclinical MRI scanner with minimal power consumption (545 mW). Our work also presents a novel method for simultaneously acquiring multinuclear NMR spectra using a single RF channel. This approach allows us to use high-sensitivity NMR signals to correct for field drift, enhancing signal averaging even in permanent magnet NMR systems that are prone to temperature-dependent drift. We believe this advancement could have broad implications for the future of NMR and MRI technology. H. Esmaeilizadshali, S. Lehmkuhl, J. Korvink, and M. Jouda, “Localized Shims Enable Low-Field Simultaneous Multinuclear NMR Spectroscopy,” Anal. Chem., Oct. 2024, https://lnkd.in/exhFW7qq

The Scientific Arab Forum Our members; Shahenda Ramadan Shahenda Ramadan Ramadan is an International Max Planck Research School (IMPRS) doctoral student in the the Dimova Lab at the Max Planck Institute of Colloids and Interfaces. Current research Ramadan employs semi-synthetic strategies involving the expression of proteins and chemo selective attachment of synthetic GPI glycolipids to obtain well-defined GPI-APs (e.g., CD59 and Thy-1). She use these proteins to investigate the properties and behaviour of GPI-APs and their interaction with other proteins on model membranes. To achieve this, she inserts GPI alone and GPI-anchored proteins into lipid vesicles of different composition and size. She then uses Surface Plasmon Resonance (SPR) and fluorescence microscopy techniques such as Fluorescence Resonance Energy Transfer (FRET) and Fluorescence Correlation Spectroscopy (FCS) to study the effect of GPI-APs on model membranes and to quantify their interactions with other proteins. Future research vision With the knowledge Ramadan gained in drug delivery and protein research from her masters and doctoral studies, she will shift focus to active drug targeting in the future. Key publications Ramadan, S. Fusogenic proteoliposomes for the delivery of functional transmembrane ion channels; a possible approach for the treatment of channelopathies. International Journal of Biological Macromolecules, 2019. Tammam, S. Repurpose but also (nano)-reformulate! The potential role of nanomedicine in the battle against SARS-CoV2. Journal of Controlled Release, 2021. Key awards Master's scholarship, Deutscher Akademischer Austauschdienst (DAAD). IMPRS-ML | International Max Planck Research School for Molecules of Life #biology https://lnkd.in/dVTVxWw2

Did you hear? Earlier this week we launched Depixus MAGNA One! As part of our launch, we’re thrilled to announce an exclusive webinar in partnership with the Biophysical Society! 📅 Date: October 9, 2024 ⏰ Time: 12:00 PM - 1:00 PM EST 🎤 Hosted by: Gordon Hamilton, CEO of Depixus Depixus MAGNA One is the world’s first large-scale magnetic force spectroscopy platform. It allows you to see biology as it really happens—offering unprecedented real-time insights into thousands of biomolecular interactions simultaneously. Our game-changing laboratory instrument can analyze individual and multi-way interactions between DNA, RNA, proteins and small molecules, including challenging targets such as RNA and protein-protein interactions. Whether you're exploring fundamental biological mechanisms or developing novel therapeutics, don’t miss this chance to explore how Depixus MAGNA One can transform your research. 👉 Register for the webinar here: https://lnkd.in/gzq-v2kx 👈 #Biophysics #DrugDiscovery #Interactomics #DrugDevelopment #Webinar 

My first #Postdoctoral paper from the lab of Katja Petzold is now online in BioRxiv. We show how #DynamicNuclearPolarisation #solidstateNMR can aid in elucidating structural information from dynamic part of microRNA in human argonaute-2: microRNA complex which is undefined in many X-ray crystal structures. Additionally, the cryogenic temperature allowed for the trapping and detecting of different room temperature dynamic states of RNA which are otherwise invisible. This will open up the field to study microRNA based regulation and get structural insights into the biologically relevant dynamic states. #research #NMR Uppsala University #MSCA https://lnkd.in/egEUxqep

Check out the recent study investigating a novel pathway for autophagy modulation. We express our deepest gratitude to the collaborative teams led by Prof. Dong Kee Yi and Prof. Seong Soo A. An for their remarkable joint achievements. The team investigated how iron oxide (Fe3O4) and silica (SiO2) nanoparticles influence autophagy. They discovered that both Fe3O4 and SiO2 nanoparticles could promote cell growth and modulate autophagy, with the combination of these nanoparticles particularly enhancing cell growth through stimulated autophagy. These findings suggest that Fe3O4 and SiO2 nanoparticles could aid in disease treatment and provide a clinical strategy for future studies by improving kidney cell proliferation and repair. In this study, LC3 expression depending on the nanoparticles was visualized using an automated live-cell imaging system, Celloger® Mini Plus. We are grateful for Celloger® Mini Plus being part of the research.   To read the full article: https://lnkd.in/g4WaRBGk More information about Celloger® Mini Plus: https://lnkd.in/g8CTm7hW #livecellimaging #Celloger #CellogerMiniPlus #Curiosis #autophage #nanoparticle

Despite the availability of various biophysical techniques to assess PROTAC interactions, researchers encounter several obstacles when employing these methods. To overcome these obstacles, scientists seek a dependable, high throughput, and precise method to assess PROTAC interactions. Discover the benefits of using a plate-based screening platform to measure binding affinities in this brochure from NanoTemper Technologies #AD: https://ow.ly/kOYu50QyuLP

Throw back to last week at LINXS Institute of advanced Neutron and X-ray Science where Claire L Lyons heard talks from Swedish and international researchers and infrastructures about how to use neutrons for drug discovery, design, and formulation research. Neutrons can be used for structure analysis, contrast imaging and multi-compositional analysis. This is useful for looking at the role of hydrogen bonding and drug interactions, liquid-aqueous interfaces, and lipid nanocarriers for mRNA delivery as well as interactions at lipid bilayers, all important for various stages of drug design and development. We heard how these experiments will be possible at European Spallation Source ERIC in the future using SANS (small angle neutron scattering), reflectometry and macromolecular crystallography. An exciting announcement was that workshops demonstrating early science at ESS will start this year, for example at Swedish Neutron Week which is on this month in Lund. https://neutronweek.site/ Already available at ESS is the Deuteration and Macromolecular Crystallisation (DEMAX) which offers both chemical and biological deuteration, which are necessary for working with many neutron experiments. Rolling access is ongoing, see the website for more information. https://lnkd.in/dSVfFtPh Contact us at InfraLife if you would like to know more about how our infrastructures can help with your Life Science research.