JITENDRA MANI TRIPATHI’s Post

Thrilled to share our recent study on the entrapment of glucose oxidase from Aspergillus niger in poly(acrylamide-co-acrylic acid) hydrogels in Elsevier Journal with IF. 3.4. This innovative approach significantly improves enzyme stability and catalytic efficiency, making it highly applicable in industries like pharmaceuticals and textiles. Excited about the potential for scaling up this research to industrial applications! Title: Entrapment of glucose oxidase from Aspergillus niger ISL-09 in poly(acrylamide-co-acrylic acid) hydrogels for improved stability and catalytic efficiency towards industrial applications https://lnkd.in/dkQEB_w9 #Biotechnology #Enyzme #Purification #Stability #IndustrialApplications

Enzyme Kinetics Simplified in 60 Seconds #enzymes #csirnetlifescience #enzymekinetics #csirnet #csirnetjune2024 #revision #unit1 #biochemistry

𝐄𝐱𝐩𝐥𝐨𝐫𝐢𝐧𝐠 𝐭𝐡𝐞 𝐕𝐞𝐫𝐬𝐚𝐭𝐢𝐥𝐢𝐭𝐲 𝐚𝐧𝐝 𝐑𝐞𝐚𝐜𝐭𝐢𝐯𝐢𝐭𝐲 𝐨𝐟 𝐀𝐜𝐢𝐝 𝐂𝐡𝐥𝐨𝐫𝐢𝐝𝐞𝐬 𝐢𝐧 𝐎𝐫𝐠𝐚𝐧𝐢𝐜 𝐒𝐲𝐧𝐭𝐡𝐞𝐬𝐢𝐬 Acid chlorides, also known as acyl chlorides, are highly reactive organic compounds characterized by the presence of a carbonyl group bonded to a chlorine atom. They are derived from carboxylic acids through a process called chlorination, where the hydroxyl group (-OH) of the acid is replaced by a chlorine atom (-Cl). This substitution greatly enhances the electrophilicity of the carbonyl carbon, making acid chlorides one of the most reactive types of organic compounds. Their reactivity stems from the electron-withdrawing nature of the chlorine atom, which polarizes the carbonyl bond and facilitates nucleophilic attack. "𝐃𝐨𝐰𝐧𝐥𝐨𝐚𝐝 𝐨𝐮𝐫 𝐬𝐚𝐦𝐩𝐥𝐞 𝐫𝐞𝐩𝐨𝐫𝐭 𝐭𝐨𝐝𝐚𝐲 𝐭𝐨 𝐞𝐱𝐩𝐥𝐨𝐫𝐞 𝐟𝐢𝐫𝐬𝐭𝐡𝐚𝐧𝐝 𝐡𝐨𝐰 𝐨𝐮𝐫 𝐢𝐧𝐬𝐢𝐠𝐡𝐭𝐬 𝐜𝐚𝐧 𝐭𝐫𝐚𝐧𝐬𝐟𝐨𝐫𝐦 𝐲𝐨𝐮𝐫 𝐛𝐮𝐬𝐢𝐧𝐞𝐬𝐬 𝐬𝐭𝐫𝐚𝐭𝐞𝐠𝐲!" @ https://lnkd.in/dgzKmiie Due to their high reactivity, acid chlorides are valuable intermediates in organic synthesis, particularly in the formation of amides, esters, and other carboxylic acid derivatives. They undergo rapid reactions with nucleophiles such as amines and alcohols to form amides and esters, respectively, under mild conditions. This reactivity also makes them useful in peptide synthesis and pharmaceutical manufacturing. However, their sensitivity to moisture and air necessitates handling under anhydrous and inert conditions to avoid undesired side reactions or decomposition. Overall, acid chlorides play a crucial role in modern organic chemistry, facilitating the synthesis of complex molecules and contributing to advancements in various fields, from materials science to pharmaceuticals. 𝐊𝐞𝐲 𝐏𝐥𝐚𝐲𝐞𝐫𝐬: BASF, Novaphene Specialities Pvt Ltd, CABB, Orion Chem Pvt Ltd, Wilmar International, TRANSPEK INDUSTRY LTD, VanDeMark Chemical Inc, Kuhlmann Europe #OrganicChemistry #ChemicalReactivity #AcidChlorides #OrganicSynthesis #Chemistry #Research #Science

Protein-Biochemistry and Overview #protein #biochemistry

𝐓𝐡𝐞 𝐒𝐞𝐜𝐫𝐞𝐭 𝐖𝐞𝐚𝐩𝐨𝐧 𝐨𝐟 𝐈𝐧𝐝𝐮𝐬𝐭𝐫𝐲: 𝐖𝐡𝐲 𝐄𝐧𝐳𝐲𝐦𝐞𝐬 𝐀𝐫𝐞 𝐨𝐧 𝐭𝐡𝐞 𝐑𝐢𝐬𝐞 #Enzymes are biological molecules that act as catalysts, facilitating and accelerating chemical reactions in living organisms. They play a fundamental role in the regulation of various metabolic processes, ensuring the efficiency and specificity of biochemical reactions necessary for life. 𝐒𝐚𝐦𝐩𝐥𝐞 𝐓𝐎𝐂: https://lnkd.in/g--MtZip 𝐆𝐞𝐭 𝐒𝐚𝐦𝐩𝐥𝐞 [𝐏𝐃𝐅]: https://lnkd.in/gDVgD3iS 𝐊𝐞𝐲 𝐟𝐞𝐚𝐭𝐮𝐫𝐞𝐬 𝐨𝐟 𝐞𝐧𝐳𝐲𝐦𝐞𝐬 𝐢𝐧𝐜𝐥𝐮𝐝𝐞: 𝐂𝐚𝐭𝐚𝐥𝐲𝐬𝐢𝐬: Enzymes function by lowering the activation energy required for a chemical reaction to occur. This enables the reaction to proceed at a much faster rate than it would without the enzyme, making biological processes more efficient. 𝐒𝐩𝐞𝐜𝐢𝐟𝐢𝐜𝐢𝐭𝐲: Enzymes are highly specific in their action. Each enzyme typically catalyzes a specific reaction involving specific substrates, and the enzyme's active site is structured to accommodate these substrates with precision. 𝐀𝐜𝐭𝐢𝐯𝐞 𝐒𝐢𝐭𝐞: Enzymes have active sites, which are specific regions where the substrates bind to undergo the catalytic reaction. The three-dimensional structure of the active site is crucial for the enzyme's specificity and efficiency. 𝐒𝐮𝐛𝐬𝐭𝐫𝐚𝐭𝐞: The substance upon which an enzyme acts is called the substrate. Enzymes recognize and bind to their specific substrates, facilitating the conversion of substrates into products during the catalytic process. 𝐋𝐨𝐜𝐤 𝐚𝐧𝐝 𝐊𝐞𝐲 𝐌𝐨𝐝𝐞𝐥: The lock and key model is a classic illustration of enzyme specificity. It describes the concept that the active site of an enzyme is like a lock, and the substrate is like a key that fits into the lock perfectly. 𝐓𝐞𝐦𝐩𝐞𝐫𝐚𝐭𝐮𝐫𝐞 𝐚𝐧𝐝 𝐩𝐇 𝐒𝐞𝐧𝐬𝐢𝐭𝐢𝐯𝐢𝐭𝐲: Enzymes function optimally within specific temperature and pH ranges. Extreme conditions can denature enzymes, altering their structure and impairing their catalytic activity. 𝐄𝐧𝐳𝐲𝐦𝐞-𝐒𝐮𝐛𝐬𝐭𝐫𝐚𝐭𝐞 𝐂𝐨𝐦𝐩𝐥𝐞𝐱: During catalysis, enzymes form temporary complexes with their substrates known as enzyme-substrate complexes. This interaction allows for the chemical transformation of substrates into products. 𝐄𝐧𝐳𝐲𝐦𝐞 𝐑𝐞𝐠𝐮𝐥𝐚𝐭𝐢𝐨𝐧: Enzyme activity can be regulated to meet the metabolic needs of an organism. Factors like feedback inhibition, allosteric regulation, and the presence of coenzymes or cofactors can influence enzyme function. DuPont Novozymes BASF Sanofi Roche #Enzymes #Biocatalysts #Biochemistry #IndustrialBiotechnology  #EnzymeApplications #BiomedicalResearch #EnzymeInnovation #EnzymeTechnology #CatalyticEfficiency #BiologicalCatalysts  #EnzymeDiscovery #BiocatalysisIndustry #BiotechAdvancements #EnzymeEngineering #MolecularBiology #EnzymaticReactions

🌟 Featured Selection Guide: PHOSPHORAMIDITE SELECTION GUIDE Our comprehensive phosphoramidite quencher guide includes ex/em data, solvents, and molecular weights, with integrated links to product pages. View or download the printable pdf https://lnkd.in/g4qYHH5Y #biotech #biochemistry #guide

The Preparations and Applications of Organophosphorus Compounds https://lnkd.in/eRaMY_qe Special Issue Information Dear Colleagues, Organophosphorus compounds are not only indispensable substances to maintain the normal existence of life but also have a wide range of applications in nucleic acids, coenzymes, organophosphorus nerve gases, organophosphorus insecticides, organophosphorus fungicides, organophosphorus herbicides, chemotherapeutic agents, plasticizers, antioxidants, surfactants, complexants, organophosphorus extractants, flotation agents, flame retardants and more. This Special Issue entitled "The Preparations and Applications of Organophosphorus Compounds" aims to present all aspects of the current original research, from synthetic methodology to catalysis, and from materials to biologically active compounds. It is my great pleasure to invite experts in the field of organophosphorus chemistry to submit manuscripts in the form of articles and comprehensive reviews to this Special Issue. Prof. Dr. K. Michał Pietrusiewicz Prof. Dr. Oleh Demchuk Guest Editors Keywords organophosphorus chemistry organophosphorus compounds phosphorus-containing heterocycles reactivity of organophosphorus compounds phosphorus-based organocatalysis phosphorus-containing drugs structure elucidation of organophosphorus compounds

💥 Heck coupling 💥reaction is a palladium-catalyzed process that forms carbon-carbon bonds by coupling alkenes with aryl or vinyl halides. 🌟Mechanism🌟 ⚡️Oxidative Addition: Palladium(0) catalyst reacts with an aryl/vinyl halide to form a palladium(II) complex. ⚡️Alkene Coordination and Insertion: The alkene coordinates to the palladium, then inserts into the Pd-C bond.β-Hydride ⚡️Elimination: Formation of the desired alkene product and a palladium-hydride complex. ⚡️Reductive Elimination: The palladium-hydride complex regenerates the active palladium(0) catalyst. Synthetic Steps⬇️ ✔️Reactants: Select appropriate aryl/vinyl halides and alkenes.Catalyst and Ligand: Use palladium(0) catalysts (e.g., Pd(PPh3)4) with phosphine ligands. ✔️Base: Add a base like triethylamine or potassium carbonate. ✔️Solvent: Common choices include DMF, toluene, or NMP. ⛲️Reaction Conditions⛲️ Conduct under an inert atmosphere. Use elevated temperatures (80-140°C). Reaction time varies from a few hours to overnight. 🌀Work-Up🌀 Cool the reaction mixture.Quench with water or dilute acid. Extract the organic phase.Dry over anhydrous sodium sulfate. Filter and concentrate. Purify by column chromatography or recrystallization. 🔷️Applications🔷️ Pharmaceuticals: Synthesis of complex drug molecules. Agrochemicals: Creation of pesticides and herbicides. Materials Science: Development of organic electronic materials. Natural Products: Construction of natural product analogs for research and development. P.S.If you like the content then follow Rohit Awasthi #OrganicChemistry #HeckReaction #SyntheticChemistry #PalladiumCatalysis #ChemicalSynthesis #Pharmaceuticals #Agrochemicals #MaterialsScience #Catalysis #ChemistryResearch #DrugDevelopment #ChemistryInnovation #ScienceAndTechnology

More details on our recent publication on photo enzymatic decarboxylation! In this study, we describe a light-driven photocatalytic decarboxylation of palmitic acid and related fatty acids using Chlorella variabilis fatty acid photodecarboxylase (CvFAP). By utilizing violet light emitting diode (LED) light (50 W; 397 nm), we achieved a remarkable conversion efficiency of 99% within just 4 min, surpassing the previous 79% conversion achieved in 60 min using blue LED light (300 W; 439 nm). Importantly, the use of 50 W violet LED light also resulted in a lower enzyme photoinactivation rate when compared to 300 W blue LED. Comparing the whole-cell biocatalyst with the enzymatic extract, we found that the former demonstrated superior catalytic performance and reduced susceptibility to photoinactivation. Furthermore, whole-cell biocatalyst reuse was demonstrated after five sequential batches. Employing this approach, we successfully synthesized 26 mmol L-1 h-1 of pentadecane, showcasing a promising strategy to improve productivity. These findings represent a significant advancement in CvFAP photodecarboxylation processes compared to the literature, utilizing an alternative light source, with potential implications to the biofuel sector

https://bit.ly/3vgInnZ - Read the Article here Analysis of Forced Degradation Products in Rilpivirine using RP-HPLC and Peak Purity Evaluation Krishna University, Machhlipattanam #Degradationproducts #Gradient #Impurities #ICH #Nonnucleoside #RilpivirineHCl #RLPAmideA #RLPAmideB #ZRLP #chemistry #biochemistry #nanomaterial #analyticalchemistry #chemicalengineering #Phytochemicals #ChemicalSciences #ChemicalTechnology