Jotbody -Beyond Affinity-’s Post

Ophthopedia Update:“Congenital Stationary Night Blindness: Structure, Function and Genotype – Phenotype Correlations in a cohort of 122 patients.”: To examine the molecular causes of Schubert-Bornschein (S-B) congenital stationary night blindness (CSNB), clinically characterize in detail, and assess genotype-phenotype correlations for retinal function and structure. #Ophthalmology #Retina #Ophthotwitter

📍 Multiscale topology classifies cells in subcellular spatial transcriptomics Benjamin et al. proposed a multiscale approach to automatically classify cell types at this subcellular level, using both transcriptomic information and spatial context. Key points ✅ Applicability to targeted and whole-transcriptome spatial platforms ✅ Improving cell classification and morphology for human kidney tissue  ✅ Pinpointing individual sparsely distributed renal mouse immune cells without reliance on image data ✅ Identifying cell spatial relationships characteristic of a mouse model of lupus nephritis. ➡ More details: https://lnkd.in/eqVyMPcR #UniversityofOxford #spatialomics #spatialbiology #singlecellanalysis #singlecell

Exciting advancements in understanding Parkinson's Disease at a cellular level! A recent study utilized single nucleus transcriptome analysis, revealing intriguing insights. Notably, our Molecular Cartography technology allowed a deep dive into the spatial dimension. Using a panel of cell type-specific markers, they confirmed cell proportions and validated findings from snRNA-seq. Spatial transcriptomics with single-cell resolution showcased the depletion of tyrosine hydroxylase (TH) in glial cells compared to neurons, offering valuable insights into dopaminergic neuron degeneration. Read the whole study here: https://lnkd.in/etES7vsT #parkinsonsresearch #molecularcartography #cellularanalysis #innovation

I want to share a very interesting preprint. It is important to notice that this is still in the preprint stages. The authors identify a neurogenic glial niche in mice that can be modulated to create new neurons in situ. This niche seems also to be present in the human aged brain. Strenghts- The possibility of a new cell source for therapeutic interventions. Accessibility for activation via the ventricles. Weaknesses- May not translate to humans Patented targets? Is the activation of this cell niche is therapeutic in aged humans? Opportunities- To bypass the need for external autologous cell sources for cell-based neurotherapeutics. MERFISH atlas can be used to identify additional populations Scalable therapy without the need for ex-vivo cell expansion Threats- Similar approaches may be readily conceived and implemented at scale. _____________ #genetherapy #genetherapies #celltherapy #cellandgenetherapy

An incredible collaborative achievement led by Allen Institute in Seattle and revealed a comprehensive cell map of a mammalian brain! 9 articles were published side by side on Nature to address the discoveries from evolutionary to molecular aspect. This information will provide a great insight for drug design and CNS disease studies! #wholebraincellcensus #alleninstitute #cnsdiseasemodel

🔬 Exploring the Iris Anterior Limiting Layer's Impact on Uveitis & Iris Tumors: 🎯 Unraveling the crucial role this layer plays in disease pathogenesis and potential breakthroughs. Join the discussion on how understanding this intricate. 🤓👁️ #Uveitis #OCTImaging #EyeHealth #MedicalResearch #ASOCT #ClinicalCases #InflammationInsights #retina #eye #ophthalmology Topcon Healthcare Young Uveitis Specialists The Ocular Immunology and Uveitis Foundation Uveitis National Clinical Study Group

Continuous cycles of muscle fiber regeneration and degeneration is a hallmark of Becker muscular dystrophy, stemming from contraction-induced damage. To better understand the process of muscle degeneration, new work led by a team at Newcastle University used Imaging Mass Cytometry™ to analyze the different cell types, extracellular matrix proteins, growth factors and markers of muscle regeneration present in muscle biopsies of BMD patients at different stages of disease severity. “IMC enables the study of changes in tissue structure along disease progression, spatio-temporal correlations and opens the door to better understand new potential pathogenic pathways in human samples.” Read the #publication in Scientific Reports: https://lnkd.in/dar2X32t #tissueimaging #proteomics #musculardystrophy

📢Interesting article: Galvanotactic Migration of Glioblastoma and Brain Metastases Cells 🎓 by Dr. Falko Lange et al., from Rostock University Medical Center, Germany. 👉Enjoy reading: https://lnkd.in/eZQMjp6N #galvanotaxis; #glioblastoma; #brain metastasis; #migration; #capivasertib; #afatinib

This is such a beautiful work! Beautiful cryo-CLEM work on the Alzheimer's pathology. 🧠 🔬 "Madeleine A. G. Gilbert et. al. used cryo-fluorescence microscopy-targeted cryo-sectioning, cryo-focused ion beam-scanning electron microscopy lift-out and cryo-electron tomography, and determined in-tissue architectures of β-amyloid and tau pathology in a postmortem Alzheimer’s disease donor brain. β-amyloid plaques contained a mixture of fibrils, some of which were branched, and protofilaments, arranged in parallel arrays and lattice-like structures. Extracellular vesicles and cuboidal particles defined the non-amyloid constituents of β-amyloid plaques. By contrast, tau inclusions formed parallel clusters of unbranched filaments. "

Multimodal profiling reveals site-specific adaptation and tissue residency hallmarks of γδ T cells across organs in mice https://lnkd.in/eX52Cpin