Mark Hilliard’s Post

A Multi-Omic, Bioanalytical LC/MS Study Mechanisms of L-asparaginase toxicity were elucidated with separations for metabolites, lipids, and peptides, and a fast, sensitive 6495D LC/TQ MS system. https://bit.ly/3IHEig5 #bioanalysis #multi-omic

A Multi-Omic, Bioanalytical LC/MS Study Mechanisms of L-asparaginase toxicity were elucidated with separations for metabolites, lipids, and peptides, and a fast, sensitive 6495D LC/TQ MS system. https://bit.ly/3IHEig5 #bioanalysis #multi-omic

Here Shah et al evaluates the functionality of diethylpyrocarbonate (DEPC)-labeling and fast photooxidation of proteins (FPOP) with MS for Biotherapeutics (Bispecific antigen-binding biotherapeutics) higher order structure functionality. 🛎 Overview: Techniques such as circular dichroism and DLS can review fast but low resolution structural data for biotherapeutics But they lack information about localized conformational changes, which can significantly impact the molecule’s efficacy and safety profile. Here Shah et al employes DEPC and FPOP via MS to bridge the information gap that classical analytical methods have. 🎯 Summary: Shah et al observed thst both techniques were able to subtle structural changes occurring at the amino acid residue level within the antigen-binding domain that was not observed with conventional methodologies ie circular dichroism. Furhermore Shah et al did note FPOP has the advantage of providing broader coverage of amino acid residues that can be oxidized. Whereas DEPC labeling is significantly simpler in its implementation. #massspectrometry #massspec #chemistry #biopharmaceuticals #biochemistry #biotech #science #sciencecommunication #scienceandtechnology #innovation #biopharma #analyticalchemistry #analytical #amgen Amgen 📌 Full publication available:

Exposing the molecular heterogeneity of glycosylated biotherapeutics The molecular heterogeneity of glycosylated biotherapeutics often complicates analysis by intact mass spectrometry. Here, the authors propose a simplified procedure for characterization that employs proton transfer charge reduction. Integration with glycomic and glycopeptide datasets can further provide glycoform-level information. https://lnkd.in/e-gKmt-5

𝐖𝐞’𝐫𝐞 𝐠𝐢𝐯𝐢𝐧𝐠 𝐚𝐰𝐚𝐲 𝐨𝐧𝐞 𝐟𝐫𝐞𝐞 𝐚𝐧𝐭𝐢𝐛𝐨𝐝𝐲 𝐜𝐡𝐚𝐫𝐚𝐜𝐭𝐞𝐫𝐢𝐳𝐚𝐭𝐢𝐨𝐧 𝐬𝐞𝐫𝐯𝐢𝐜𝐞 𝐭𝐨 𝐨𝐮𝐫 𝐟𝐨𝐥𝐥𝐨𝐰𝐞𝐫𝐬! Why? We’ve launched new LC-MS services and we want the world to know: ➡ Peptide Mapping ➡ Glycan analysis ➡ Disulfide bond analysis ➡ Intact mass analysis and more! 𝐖𝐡𝐚𝐭 𝐲𝐨𝐮 𝐰𝐢𝐧: One of the above LC-MS characterization services for an antibody. See promotion details here: https://lnkd.in/gZ3CTHkr 𝐇𝐨𝐰 𝐭𝐨 𝐰𝐢𝐧:  1. Follow Rapid Novor on LinkedIn 2. “Like” and “Share” this post to your feed! 3. Stay tuned for the announcement on July 31 #AntibodyCharacterization #AntibodyDevelopment

The first work in 2024 was now published by Biochemistry. This is my second work writing as a first author in protein modification field. I hope we can continue to provide updates about cover art as well! While we have been continuing with antibody modifications, this is our first time attaching a payload to an ADC as well as observing biological activity on it. It has been an exciting project. https://lnkd.in/gsrwgTjM In 2021, biological evaluations were conducted for the tag-free antibody modification method using LplA. The ADCs targeting HER2 showed promising results in terms of bioactivity and safety. Further research is needed for in vivo evaluations and safety assessments, but utilizing LplA for ADC production holds great potential in biopharmaceutical manufacturing.

A Multi-Omic, Bioanalytical LC/MS Study Mechanisms of L-asparaginase toxicity were elucidated with separations for metabolites, lipids, and peptides, and a fast, sensitive 6495D LC/TQ MS system. Watch presentation: https://bit.ly/3IHEig5

Learn the differences and advantages of the Gyrolab® platform compared to standard ELISA for reducing matrix interference, manual labor, reagent consumption and assay incubation time. Catch up on our latest webinar in partnership with Gyros Protein Technologies here >>> https://bit.ly/3NWgTum

At #AMSbiopharma we have developed the necessary and specific tools for detailed structural characterizations: PEG heterogeneity, site of addition, and the number of attached PEGs as is included in ICH Q6B. Please take a look at what we can do to help you with the characterization of your specific PEGylated proteins! 👇 #Biopharmaceuticals https://lnkd.in/danKXvF3

Reduce variability in therapeutic #oligonucleotide analysis with our automation-friendly OligoWorks SPE Kits. Register for our webinar to learn how to obtain robust #bioanalytical data, efficiently. https://bit.ly/3SjxI3x #biotech