How to purify lentivirus,
Upstream to final formulation 👇
1) 𝐂𝐞𝐥𝐥𝐮𝐥𝐚𝐫 𝐃𝐢𝐫𝐞𝐜𝐭𝐢𝐯𝐞 𝐃𝐢𝐬𝐩𝐚𝐭𝐜𝐡
Cells receive marching orders to kickstart lentivirus production.
2) 𝐓𝐡𝐞 𝐇𝐚𝐫𝐯𝐞𝐬𝐭 𝐇𝐚𝐮𝐥
Gathering lentivirus amidst a motley crew of impurities.
3) 𝐒𝐢𝐞𝐯𝐢𝐧𝐠 𝐒𝐜𝐢𝐞𝐧𝐜𝐞
Filtering out oversized troublemakers, while smaller nuisances linger.
4) 𝐂𝐡𝐫𝐨𝐦𝐚𝐭𝐨𝐠𝐫𝐚𝐩𝐡𝐲 𝐂𝐡𝐨𝐫𝐞𝐨𝐠𝐫𝐚𝐩𝐡𝐲
Lentivirus leads the column dance, separating from most interlopers; a few clingy ones remain.
5) 𝐏𝐮𝐫𝐢𝐟𝐢𝐜𝐚𝐭𝐢𝐨𝐧 𝐏𝐫𝐨𝐭𝐨𝐜𝐨𝐥
Through meticulous washing and filtration, the lentivirus elite emerges, bidding farewell to lingering contaminants.
6. 𝐓𝐡𝐞 𝐅𝐨𝐫𝐦𝐮𝐥𝐚𝐭𝐢𝐨𝐧 𝐅𝐢𝐧𝐚𝐥𝐞
Lentivirus finds sanctuary in a stabilising buffer, poised for storage until its scientific spotlight shines once more
Voilà! Lentivirus purification, where science meets the dance floor!
Thanks to Benjamin McLeod for helping here. Follow him for insights.
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Product Manager and Biologist. Passionate about Biologics, Cell and Gene Therapies, Microfluidics, SPR, Lab Automation and Drosophila
1moHahaha amazing! 🧫❤️