Molecular Designs’ Post

🧬Dive into our range of real-time PCR master mixes! At Chemie Brunschwig AG , we are proud to offer NZYtech's cutting-edge products, designed to meet the needs of molecular biology research. 🔬 The offerings cover two fundamental nucleic acid types 👉 DNA and RNA. Each is designed to deliver optimal performance and precision in your experiments. 💡 Within each category, we provide two distinct formulations: ✅ probe-based ✅ intercalating dye-based master mixes These variations cater to different assay requirements, ensuring flexibility and accuracy in your experimental designs. Whether you are probing for specific sequences or utilizing intercalating dyes for broader detection, our master mixes offer the versatility you need to achieve reliable results. Moreover, our product line distinguishes between: ✅ NZYSpeedy version (designed for swift amplification with rapid results) ✅ NZYSupreme version (characterized by its hot-start feature, guaranteeing enhanced specificity and sensitivity)   Whether you seek expedited amplification or elevated performance with hot-start capabilities, our real-time master mixes empower your research endeavors with reliability and efficiency. Browse the available documentation below to help you navigate and better understand key characteristics of our products: ⬇️   Discover how our products can streamline your experiments and lead to significant breakthroughs! #pcr #molecularbiology #scientificresearch

It is commonly known that the #PCR (#PolymeraseChainReaction) is an important technique in #molecularbiology, considering its ability to amplify #DNA sequences. But are you familiar with all the phases involved in this #technique? - Phase I: “Denaturation”, in which high temperatures disrupt #hydrogen bonds between complementary DNA strands, transforming the double-stranded DNA into single strands. - Phase II: “Annealing”, in which #primers bind to their complementary sites on the single-stranded DNA template. In this phase, the reducement in temperature facilitates primer annealing. - Phase III: “Extension”, in which the DNA polymerase extends the primers by adding nucleotides complementary to the template strand, resulting in the #synthesis of two new DNA strands, each complementary to the original template, effectively doubling the DNA content. REFERENCES Bhat, A. I., et al. “Polymerase chain reaction." (2020): 323-345. Delidow, B. C., et al. (1993). “Polymerase Chain Reaction”. In: White, B.A. (eds) PCR Protocols. Methods in Molecular Biology, v. 15. Humana Press, Totowa, NJ. PraxiLabs - Available in: https://lnkd.in/dxDVwq-T

Accurate DNA quantification is key for many molecular biology workflows. Without precise knowledge of your sample's concentration, yield and purity, the success of downstream applications can be put at risk. This article describes the different methods available for quantifying DNA, highlighting their strengths, weaknesses and the essential equipment you'll need to perform them. Please note that most of the techniques explained in this article can also be used if you want to quantify RNA instead of DNA. https://lnkd.in/eT6huq2S This article written by Éva Mészáros is part of a series of 5 blogs, explaining how to get from samples to pure DNA  To find out more, click here

Speed, accuracy, and reliability in one mix! 🚀 Vazyme 2 x Phanta Flash Master Mix (Dye Plus), with ~106X higher fidelity than Taq DNA Polymerase and flash amplification up to 5 sec/kb, ensures robust performance and broad compatibility. It is applicable for amplification reactions of genomic DNA, cDNA, dU-containing DNA, and crude samples as templates. Optimize your workflow and achieve accurate, fast, and reliable DNA amplification now>>https://bit.ly/3VxOoGg #lifescience #biotech #PCR #geneticresearch #molecularbiology 

Purifying #DNA is a common process in molecular biology. Unlike DNA extraction, it doesn't include any lysis steps to break the cell membrane and liberate the DNA. Instead, it involves the clean-up of your samples, e.g. to effectively remove all components that were used to facilitate amplification of the target sequence during a polymerase chain reaction (#PCR). Various DNA purification methods are available and this article will provide a detailed comparison of their advantages, disadvantages, and applications. Read on to learn how to ensure that your samples are pure enough for your downstream application: https://lnkd.in/eYg_g6Kx

Excited to share our recent work on developing a #bioanalytical method to analyze low-abundant #glycolipid-anchored proteins or #GPI-APs within live cells. https://lnkd.in/ewsDFuui 🔴 The highlight of this research is leveraging DNA #hybridization #HCR for #signal-amplification allowing visualization of cell surface #GPI-APs. This method minimally perturbs cellular physiology, making it highly compatible for use in live cells. 🔴 #GPI-APs are essential but low-abundant proteins that play crucial roles in immunological regulation. Their low abundance has hindered detailed investigations of these biomolecules. Previously, we used metabolic #glycoengineering to label these proteins, which required large quantities of chemical probes. 🔴 Our new method can label proteins with as little as nanomolar quantities of the probe. This method will be highly applicable to study this proteins within live cell surface using modern imaging techniques. I extend my gratitude to Mohit jaiswal, Dr. Zhongwu Guo and the entire team for their contributions in making this work possible. #Glycobiology #Analyticalchemistry #glycan

PCR (Polymerase Chain Reaction) is a widely used molecular biology technique that allows for the amplification of specific DNA sequences. The process involves three main steps: denaturation, annealing, and extension. In denaturation, the DNA is heated to high temperatures to separate its two strands. During annealing, short sequences of single-stranded DNA called primers bind to complementary sequences on the template DNA. The final step, extension, involves Taq polymerase, a heat-stable enzyme, which synthesizes new DNA strands by adding nucleotides (dNTPs) to the primers. Several components are essential for PCR to work. These include the template DNA, which contains the sequence to be amplified, primers that define the start and end points of the target sequence, Taq polymerase that builds the new DNA strands, and dNTPs (deoxynucleotide triphosphates), the building blocks of DNA. A buffer solution is also necessary to maintain the optimal pH and ionic environment, while MgCl₂ is added to activate the polymerase. Together, these components allow for the rapid and efficient amplification of DNA, making PCR a critical tool in fields like diagnostics, genetics, and forensic science. Chetna Ralh #PCR #ClinicalMicrobiology #MolecularDiagnostics #MedicalResearch #Biotech #Healthcare

𝗥𝗲𝘀𝗲𝗮𝗿𝗰𝗵 𝗦𝗻𝗶𝗽 𝟮.𝟳𝟮: This study introduces an improved method to detect special #DNA structures called G-quadruplexes (#G4s), which are common in the #genome but their function and evolution is not well understood. The findings show that G4s can be captured from live #cells by making their #membranes permeable and using #antibodies that specifically bind to these structures. The study also found that the human protein CGGBP1 reduces G4 formation at specific DNA regions. The method shows that proteins binding to DNA can interfere with G4 formation, providing new insights into how these structures are regulated within cells. Read the full paper here: https://lnkd.in/gTymaNqs #IITGNResearchSnips #Research #IITGNResearchers

Explore the intricacies of single-cell biology using highly sensitive timsTOF mass spectrometers. Delve into our dedicated single-cell webpage for insights into this complex field and discover valuable resources. Gain a deeper understanding of the application here: https://buff.ly/3WJ5gw2. #singlecell #proteomics #massspectrometry #timsTOF

I am glad to share that the part of my Ph.D. work recently published in the journal of "ACS Chemical Biology". Here, we report the synthesis of a new micro-environment-sensitive dual-labeled ribonucleoside analog to investigate the RNA structure. The ribonucleotide serves as an effective biophysical tool to probe RNA structure using fluorescence and 19F NMR techniques. The probe efficiently monitors antibiotics-induced conformational changes in the bacterial A-site RNA. Data indicates that the probe could be useful to set up a screening platform to identify new RNA binding ligands. https://lnkd.in/dkAHGtvQ