NEJM Group’s Post

Enhancing cell therapy by selecting cells with high extracellular vesicle secretion: Ke Cheng at Columbia University, Dino Di Carlo at UCLA and collaborators utilized nanovial technology to enrich millions of single cells based on EV secretion, applying this approach to select mesenchymal stem cells with high EV secretion for improved therapeutic treatment https://lnkd.in/gGt2FTNP They found that the selected MSCs exhibited distinct transcriptional profiles linked to EV biogenesis and vascular regeneration, and maintained high levels of EV secretion after sorting and regrowth. An article also authored by Doyeon Felis Koo, Xiao Cheng, Shreya Udani, SEVANA BAGHDASARIAN, Dashuai Zhu, Junlang Li, Brian Hall, Natalie Tsubamoto, Shiqi Hu and Jina Ko #extracellularvesicles #exosomes #MSCs #flowcytometry #bioengineering #Vesiculab

Just published! This work demonstrates the feasibility of producing stem cell derived extracellular vesicles in scalable bioreactors under clinically relevant serum-free, physioxic conditions, for stimulating vessel formation in brain endothelial cells - relevant to treatments for cerebral ishemia due to stroke, TBI https://lnkd.in/gEnsEJ7e

𝐁𝐞𝐲𝐨𝐧𝐝 𝐭𝐡𝐞 𝐏𝐞𝐭𝐫𝐢 𝐃𝐢𝐬𝐡: 𝐄𝐯𝐨𝐥𝐮𝐭𝐢𝐨𝐧 𝐨𝐟 𝐭𝐡𝐞 𝟑𝐃 𝐂𝐞𝐥𝐥 𝐂𝐮𝐥𝐭𝐮𝐫𝐞 𝐌𝐚𝐫𝐤𝐞𝐭 𝟐𝟎𝟑𝟑 3D cell culture is a type of cell culture that uses three-dimensional (3D) scaffolds to support cell growth and proliferation. 3D cell culture systems provide a more physiologically relevant environment for cells to grow and proliferate in vitro, as compared to two-dimensional (2D) cell culture systems. 3D cell culture systems can be used to study a variety of cell types, including cancer cells, stem cells, and organoids. 𝗥𝗲𝗾𝘂𝗲𝘀𝘁 𝗦𝗮𝗺𝗽𝗹𝗲:https://lnkd.in/dDRaacsJ 𝐏𝐫𝐨𝐦𝐢𝐧𝐞𝐧𝐭 𝐏𝐥𝐚𝐲𝐞𝐫𝐬 𝐢𝐧 𝐭𝐡𝐞 𝟑𝐃 𝐂𝐞𝐥𝐥 𝐂𝐮𝐥𝐭𝐮𝐫𝐞 𝐌𝐚𝐫𝐤𝐞𝐭 REPROCELL PromoCell Thermo Fisher Scientific Lonza Avantor 3D Biotek, LLC InSphero Lena Biosciences CN Bio #3DCellCulture #TissueEngineering #BiomedicalResearch #CellularMicroenvironments #Organoids #StemCellCulture #RegenerativeMedicine #LabOnAChip #SpheroidCulture #PrecisionMedicine

This study provides some new insights into the molecular mechanism underlying age-related adipose stem cell (ASC) dysfunction, highlighting DUXAP10 and miR-214-3p as potential rejuvenating factors for aged stem cells. The finding is interesting and introduces potential novelty in the context of wound repair ability of ASCs. https://lnkd.in/eFpGHhz5

Stem cells are attractive candidates for the regeneration of tissue and organ. Mesenchymal stem cells (MSCs) have been extensively investigated for their potential applications in #regenerativemedicine and cell therapy. For developing effective #stemcelltherapy, the mass production of consistent quality cells is required. The cell culture medium is the most critical aspect of the mass production of qualified stem cells. Classically, fetal bovine serum (FBS) has been used as a culture supplement for MSCs. Due to the undefined and heterologous composition of animal origin components in FBS, efforts to replace animal-derived components with non-animal-derived substances led to safe serum free media (SFM). Read More: https://lnkd.in/gA4vcs8Q #cellculture #primarycells #MSCs #stemcells #crueltyfree #kosheeka

September marks Spinal Cord Injury Awareness month! In recognition of this month, we're highlighting the current progress in the field. Although spinal cord injury leads to significant challenges in the day-to-day life and functioning of affected individuals, current industry innovators are working on a wide range of supporting therapies, ranging from physical exercise, drug treatments, or innovative stem cell therapies to promote regeneration of the damaged spinal cord. To help industry innovators accelerate this research faster to the ones who need it the most - the patients - researchers can make use of InnoSer's preclinical research services. This includes research models to study the efficacy of novel compounds on spinal cord injury and regeneration. Learn more about how InnoSer's spinal cord injury research model may be used in your preclinical research here: https://lnkd.in/e5BtJ2eE. Additionally, InnoSer's spasticity research model may represent another translationally relevant model for your drug development: https://lnkd.in/evyDp8Xd. #SCIAwareness #Spasticity #SCIMouseModel #SpaMouseModel #SCI #SpinalCordInjuryAwareness #SpinalCordInjury

Esco co2 incubator the safest way to grow your beautiful cells features (1) direct heat and air jacket (2) ULPA filter (3) 90°C moist heat decontamination cycle (4)180°C high heat sterilization (5) Isocide antimicrobial powder coating APPLICATIONS Stem cell research In vitro fertilization Tissue engineering Cancer research Mammalian cell

Overview of developmental pathways of various hematopoietic cells from pluripotential bone marrow stem cells. ⁉

𝗔 𝗰𝗵𝗮𝗻𝗴𝗲 𝗼𝗳 𝗵𝗲𝗮𝗿𝘁: 𝗳𝗿𝗼𝗺 𝘀𝘁𝗲𝗺 𝗰𝗲𝗹𝗹𝘀 𝘁𝗼 𝗯𝗲𝗮𝘁𝗶𝗻𝗴 𝗵𝗲𝗮𝗿𝘁 𝗰𝗲𝗹𝗹𝘀 💓 Even after almost a decade of working with induced pluripotent stem cells, every time I see cardiomyocytes spontaneously contracting in the lab I get as excited as the first time. These beating cells are a powerful reminder that ‘heart’ work does pay off! This video shows cardiomyocytes from my most recent differentiation, which then I used to create cardiac organoids for drug discovery experiments. #iPSC #Cardiomyocytes #RegenerativeMedicine

The recent study by Song et al. (2024) investigated the efficacy of applying radiation-treated umbilical cord mesenchymal stem cells (UCMSCs) via high oxygen-permeable hydrogel lenses in treating corneal alkali burns. Results showed that the treatment significantly reduced inflammation and neovascularization while promoting corneal injury repair. The study suggests that the therapeutic effect involves the suppression of the T helper 17 (Th17) cell differentiation pathway. Molecular analyses revealed changes in cytokine expression, with downregulation of IL-17 and upregulation of IL-4 and STAT1. Overall, the findings indicate that irradiated UCMSC-coated lenses inhibit corneal angiogenesis and inflammation, potentially through modulation of Th17 cell differentiation.   Read more: https://lnkd.in/dn4-Njc9 #umbilicalcord #regenerativemedicine #stemcells #research #stemcellbanking #burns #alkali