Nuclera’s Post

APP NOTE: #HuProt™ Proteome Microarrays are fabricated by immobilizing 21,000+ individually purified GST-fused human proteins on a single glass slide. Unlike nucleic acids, many proteins can become unstable after purification; therefore, correct folding of proteins prior to immobilization on a solid surface is crucially important for maintaining their physiological activities. The choice of protein expression system greatly influences the folding and post-translational modifications of proteins and can affect the success rate of protein purification.   In this app note, we explain the advantages of a yeast expression system when compared to E. coli and cell-free expression systems. In short, a yeast system provides a eukaryotic environment conducive to proper protein folding and stability, with expression of necessary chaperones and co-factors to prevent aggregation and support translation of large molecular weight proteins. View and download PDF: https://lnkd.in/gdq5pJDu #proteomics #targetid #seromics #biotech #antibodies #autoantibodies

𝐖𝐢𝐥𝐥 𝐆𝐨𝐨𝐠𝐥𝐞 𝐃𝐞𝐞𝐩𝐌𝐢𝐧𝐝'𝐬 𝐀𝐥𝐩𝐡𝐚𝐏𝐫𝐨𝐭𝐞𝐨 𝐛𝐞 𝐭𝐡𝐞 𝐧𝐞𝐱𝐭 𝐠𝐚𝐦𝐞-𝐜𝐡𝐚𝐧𝐠𝐞𝐫? Many of you are likely familiar with #AlphaFold, which had a significant impact (and sparked debate) in protein structure prediction. #AlphaProteo is an AI-based method for generating protein binders for selected target proteins. According to the original report, for the seven target proteins tested, it exhibited up to 300-fold higher binding affinity than existing methods. More information and further progress in this direction will help us tackle the challenging field of protein-protein binding. Image courtesy : DeepMind Original source: https://lnkd.in/dG4Jmqg4

Comparison between BCA and Bradford Assay BCA protein assay and the Bradford protein assay are both popular methods for protein quantification, but they have different advantages and are suited for different applications. Here, we highlight why the BCA assay might be considered better than the Bradford assay in certain contexts. #PrimaNexus #BCAproteinassay #Bradfordproteinassay #Research

Our CEO, Michael Chen, spoke with Biocompare on the considerations required when choosing a platform for protein production. The article, “Transient Protein Expression Gaining Ground” reviews traditional transient protein production platforms and the pros and cons of using prokaryotic or eukaryotic cells. Although cell-based systems are tried and true there is an accelerated interest in cell-free expression systems, like Nuclera’s eProtein Discovery™. Michael comments: ‘Ultimately, the choice between cell-free and cell-based expression is not binary. Cell-free systems excel at screening, and cell-based systems excel at scaling. The key is to understand your protein requirements and use the appropriate tools’. Read the full article here: http://eprote.in/uvtJSTNz

The latest in protein barcoding has arrived, a new preprint is now available! Learn how protein barcoding has emerged as a transformative tool for the multiplexed identification and characterization of proteins, providing a mechanism for precise tracking of protein affinity, location, and expression. Download the preprint here: https://bit.ly/4264Geh #ProteomicEra #QSI #NGPS #ProteinBarcoding #Innovation #ProteinSequencing

Structure-based protein and small molecule generation using EGNN and diffusion models: A comprehensive review. Read the article here: https://lnkd.in/gSyvbTe9

Webinar - ALTO digital SPR Key topics: *Introduction to transmembrane protein characterization *Overview of the Alto digital SPR system *Case studies demonstrating label-free characterization of challenging transmembrane proteins Registration at : https://lnkd.in/dX_4ruif ALTO technology : https://lnkd.in/dJzdnqnZ

JUST PUBLISHED: Intracellular Protein Delivery: Approaches, Challenges, and Clinical Applications Click here to read the latest free, Open Access article from BMEF: https://lnkd.in/e3AgHh9P

📝 [WHITE PAPER] Explore the bioluminescent detection of the #HiBiT #proteintag for quantifying surface protein expression. 🔄 This white paper outlines a method to measure both extracellular and total protein levels, providing insights into #proteintrafficking and #membraneprotein analysis. 👉 Read the full paper to learn more.https://bit.ly/3Y8Mdf2

Targeted protein degradation Targeted protein degradation involves redirecting protein recycling systems in our cells to destroy the disease-causing proteins. Protein degraders work by capturing the disease-causing protein and making it stick like a glue to the cellular protein-recycling machinery, which then tags the protein as expired in order to destroy it. The tag is a small protein called ubiquitin, which effectively gets fired at the disease-causing protein like a bullet. In order for the process to work effectively, ubiquitin must hit the right spots on the target protein so that it gets tagged effectively. The new work by the team enables them to see how the bullet hits the proverbial bull’s eye. Working with a protein degrader molecule called MZ1, and using high-end mass spectrometry, they were able to identify exactly where on the target protein the vital ‘tags’ are added. The work shows how degrader drugs hold onto and position disease-causing proteins, making them good targets for receiving ubiquitin molecules (ie. "ubiquitin-atable") which then leads to their destruction inside the cell. #ScienceMission #sciencenewshighlights https://lnkd.in/gTFnHJUF