Parean Biotechnologies’ Post

Exciting period for us over the past months with several papers (5+ !) published mentioning our Parean Biotechnologies team ! A new one here, on gamma delta T cells, exciting topic showcased by several biotech during conferences in London I attended early this year. Saying that we develop and master single cell and immune repertoire omics data science in various immunological & tumor settings is great....proving it by being cited as co authors of published papers with amazing academic researchers is even better !! Proud of our team ! 💪 More papers soon hopefully, stay tuned !

New Paper published on Gamma Delta T cells in PLOS Pathogens! Parean Biotechnologies team, Pierre Barennes, Valentin Quiniou and Hang Phuong PHAM are listed as co-authors, on this newly published paper, from Nathalie YARED & Myriam Capone, on long-lived memory gamma delta T-cells. Congrats to all authors ! This work is part of our contribution to Horus Project, and our data science workflow development since several months on rare populations such on gamma delta T cells, with scRNAseq, scTCRseq and scATACseq. More news coming soon on that matter ! "The involvement of γδ TCR-bearing lymphocytes in immunological memory has gained increasing interest due to their functional duality between adaptive and innate immunity. γδ T effector memory (TEM) and central memory (TCM) subsets have been identified, but their respective roles in memory responses are poorly understood. In the present study, we used subsequent mouse cytomegalovirus (MCMV) infections of αβ T cell deficient mice in order to analyze the memory potential of γδ T cells. As for CMV-specific αβ T cells, MCMV induced the accumulation of cytolytic, KLRG1+CX3CR1+ γδ TEM that principally localized in infected organ vasculature. Typifying T cell memory, γδ T cell expansion in organs and blood was higher after secondary viral challenge than after primary infection. Viral control upon MCMV reinfection was prevented when masking γδ T-cell receptor, and was associated with a preferential amplification of private and unfocused TCR δ chain repertoire composed of a combination of clonotypes expanded post-primary infection and, more unexpectedly, of novel expanded clonotypes. Finally, long-term-primed γδ TCM cells, but not γδ TEM cells, protected T cell-deficient hosts against MCMV-induced death upon adoptive transfer, probably through their ability to survive and to generate TEM in the recipient host. This better survival potential of TCM cells was confirmed by a detailed scRNASeq analysis of the two γδ T cell memory subsets which also revealed their similarity to classically adaptive αβ CD8 T cells. Overall, our study uncovered memory properties of long-lived TCM γδ T cells that confer protection in a chronic infection, highlighting the interest of this T cell subset in vaccination approaches." Marwan TOUATI Valentin Quiniou Hang Phuong PHAM Pierre Barennes https://lnkd.in/epeNgV2j

Parean Biotechnologies is now listed on Scientist.com platform ! Many of our customers ask us to register on this platform, to comply with their internal process, so...here it is ! Here are the link to our company profile: https://lnkd.in/e9a-Srdj Get access to all our services such as cytometry and cell sorting using dextramers and custom panels, single cell RNAseq, immune profiling data science, ... Marwan TOUATI Valentin Quiniou Hang Phuong PHAM Pierre Barennes

Congrats to Marius Bredon, Harry Sokol, our cofounder Hang Phuong PHAM and all coauthors for this new paper in Oncoimmunology ! A great new example of our expertise in data science, applied to microbiota, oncology & immunology. Thanks to this amazing research team for their renewed trust in our services and technologies. "Gut microbiota impacts responses to immune checkpoint inhibitors (ICI). A high level of Faecalibacterium prausnitzii have been associated with a positive response to ICI in multiple cancer types. Here, based on fecal shotgun metagenomics data, we show in two independent cohorts of patients with non-small cell lung cancer and advanced melanoma that a high level of F. prausnitzii at baseline is positively associated with a better clinical response to ICI. In MCA205 tumor-bearing mice, administration of F. prausnitzii strain EXL01, already in clinical development for Inflammatory Bowel Disease, restores the anti-tumor response to ICI in the context of antibiotic-induced microbiota perturbation at clinical and tumor transcriptomics level. In vitro, EXL01 strain enhances T cell activation in the presence of ICI. Interestingly, oral administration of EXL01 strain did not induce any change in fecal microbiota diversity or composition, suggesting a direct effect on immune cells in the small intestine. F. prausnitziistrain EXL01 will be evaluated as an adjuvant to ICI in multiple cancers in the near future." Marwan TOUATI Valentin Quiniou Hang Phuong PHAM Pierre Barennes Harry Sokol Alexis Vandier Claude Bernard

Congrats to our colleague Pierre Barennes, co-authors of this new paper in Cell Reports ! Pierre is deputy of our CSO Valentin Quiniou, and is deeply involved in new bioassay development, TCR sequencing Methods, and AI-based TCR discovery tool. He spent several years in an academic research team, triggering several papers on TCR repertoire analysis & predictive models. "Enhancing comparative T cell receptor repertoire analysis in small biological samples through pooling homologous cell samples from multiple mice Accurate characterization and comparison of T cell receptor (TCR) repertoires from small biological samples present significant challenges. The main challenge is the low material input, which compromises the quality of bulk sequencing and hinders the recovery of sufficient TCR sequences for robust analyses. We aimed to address this limitation by implementing a strategic approach to pool homologous biological samples. Our findings demonstrate that such pooling indeed enhances the TCR repertoire coverage, particularly for cell subsets of constrained sizes, and enables accurate comparisons of TCR repertoires at different levels of complexity across T cell subsets with different sizes. This methodology holds promise for advancing our un- derstanding of T cell repertoires in scenarios where sample size constraints are a prevailing concern." Congrats to Vanessa Mhanna, and all authors. Valentin Quiniou Hang Phuong PHAM Marwan TOUATI