Rafi Pelossof’s Post

#ParticularInterestPaper   📝 Expression Profile of mRNAs and miRNAs Related to the Oxidative-Stress Phenomenon in the Ishikawa Cell Line Treated Either Cisplatin or Salinomycin — Januszyk, et al.   The aim of this study was the assessment of gene expression profile changes, and to determine the miRNAs regulating genes’ transcriptional activity in an Ishikawa endometrial cancer culture exposed to cisplatin or salinomycin, compared to a control culture.   Full text is available 👇 https://lnkd.in/d_AYQ3Br   #medicine #health #research #science

Overcoming immunogenicity of a therapeutic cell product is one of the major hurdles to clear during Cell Therapeutic Development. One approach to overcoming this barrier is by using StemEdit Clinical Gene Editing Service to abrogate the expression of HLA in your therapeutic cells by knocking out the B2M and CIITA genes in the starting iPSCs. As part of our validation for the StemEdit service, we had created the B2M/CIITA double knockout in one of our iPSC lines. FACS analysis of the mutant iPSCs reveals that HLA Class I expression is reduced by 99%. Find out more about this case study and our StemEdit service: https://hubs.li/Q02h13MB0

Discover how TruGraf is revolutionizing post-transplant care with the help of Dr. Marvin, a transplant surgeon at Geisinger Medical Center. Watch 'Using Gene Expression Profile Biomarkers as an Immunosuppression Guide in Post-Transplant Care' to learn how Dr. Marvin uses TruGraf to titrate immunosuppression management, and see how it could benefit your patients too. #Eurofins #KidneyTransplantation

📍 A pan-cancer single-cell RNA-seq atlas of intratumoral B cells    Fitzsimons et al. generated a comprehensive pan-cancer scRNA-seq atlas of tumor-infiltrating B and plasma cells, capturing the full spectrum of the lineage with respect to broad-level clustering and individual gene expression profiles.    Highlights  🔸A scRNA-seq atlas identifies ten clusters of intratumoral B and plasma cells  🔸Specific B cell subsets are associated with checkpoint inhibitor therapy responses  🔸Atlas-derived B cell gene signatures validated in spatial analyses  🔸Publicly available Shiny tool enables users to interactively explore the atlas. ➡️ More details: https://lnkd.in/eMqeSEXV #spatialomics #spatialbiology #singlecellanalysis #singlecell

One week to go for #ESGCT! Many years ago I heard from a potential customer to services I was promoting saying: "I need an early success or an early failure: either one will tell me what to do next"...so WHAT ARE YOU WAITING FOR...? ...is your bioanalysis service provider taking too long to provide critical information regarding your precious clinical/pre-clinical samples? ...are you unsure about the selected method of sample preparation for your #biodistribution study...? ...have you been made aware how nucleic acid #extraction, the assay design, and #optimization process are can all have impact on the #quality of the results you have contracted to have...? ...do you need to have #answers sooner rather than later...? If any of these questions made you think, reach out or better: come to #Rome and meet the TATAA Biocenter team: we are at booth #A02! ...AND DON'T WAIT FOR THE REGULATORS TO CALL/EMAIL YOU, it will probably be too late ... #BETTERLATETHANNEVER #research #medicine #cancer #cro #glp #quality #lifesciences #drugdiscovery #pharma #biotech #biopharma

#Article: The rapid development of in vitro transcribed (IVT) RNA drugs for preventing infectious diseases and treating cancer poses both opportunities and challenges. Analytical hurdles vary, but advancements in tools are enabling progress. Kerstin Pohl, Senior Global Manager of Cell and Gene Therapy and Nucleic Acids here at SCIEX, shares insights on the emerging RNA modalities in a new article in Manufacturing Chemist 👉 https://bit.ly/47Yf7Qy #SCIEXBiopharma #RNA #DrugDevelopment

One crucial analytical technique that is highly relevant in the RNA field is capillary electrophoresis (CE). Compared with LC methods, CE-based methods show significantly better resolution of large RNA species and can be used to determine purity and integrity, marking a significant improvement in terms of resolution, reproducibility and automation capability; plus, state-of-the art instrumentation allows for temperature control to prevent the formation of secondary structures. The technology is suitable for linear mRNA, saRNA species and circRNA. Check out the link below to see how SCIEX is helping scientist battle these challenges! #sciex #rna #genetherapy #biopharma

This early warning system provides a significant time advantage over traditional screening methods, potentially allowing for more effective preventive measures and treatment strategies. The test focuses on subtle changes at the molecular level, capturing the initial stages of cellular transformation towards cancer. By monitoring shifts in gene expression patterns associated with precancerous conditions, we can flag potential issues long before they manifest as visible structural changes or abnormal cytology results. https://lnkd.in/gW6akYv2 #ceevicalcancer

Hear what Dr. Marvin, Transplant Surgeon at Geisinger Medical Center, had to say about how he uses TruGraf to help titrate immunosuppression management in this clip from the webinar: Using Gene Expression Profile Biomarkers as an Immunosuppression Guide in Post-Transplant Care. #Eurofins #KidneyTransplantation

🧬 Elevate Your #CellGeneTherapy With Cutting-Edge PCR. Rely On Our 🔬 #qPCR, 💧 #ddPCR, And 🌈 #NanoDrop Expertise To Propel Your Groundbreaking Research From Bench To Bedside. 🛠️ Bespoke DNA PCR: Hundreds Of Tailored And Ready-To-Use #TaqMan Assays For Consistent And Reliable Data To Evaluate Your Therapeutics 🎯 Comprehensive CNV ASSESMENT: Absolute #CopyNumber Variation Assays Reveal Complex #Pharmacokinetics Or Genetic Intricacies Of Disease 🦠 Precise VCN ANALYSIS: Quantitative #ViralVector #Biodistribution Studies Facilitate Safe, Therapeutic Dosing Of Novel Therapeutic Modalities 🔍 Targeted BIOMARKER TESTING: #ctDNA, Other #CancerBiomarkers, Or #Pharmacodynamic Analysis In Humoral Fluids, Cells, And Tissues ✨ Speak To Our Scientists Today And Parter With NorthEast BioLab, Where Pioneering PCR Bioanalysis Meets Unparalleled Cell & Gene Therapy Precision. https://lnkd.in/dw2ra3KM