Polina Chowdhury’s Post

Certificate of participation in the molecular docking Simulation workshop Teilnahmebescheinigung am Molecular Docking Simulation Workshop: Theoretical and Practical an vier aufeinanderfolgenden Tagen an der Tabriz University of Medical Sciences, Iran Teaching the basic concepts and how to search genomes from the reference and gene bank site, teaching how to work with autodock, Chimera, Discovery studio software and also interpreting the data results. 💻🧬🔬📚

🔬 A Vutara super-resolution microscope helped researchers Lexy von Diezmann and Ofer Rog develop a new live imaging protocol that enables the visualization of live dynamics in C.elegans gonads for up to an hour following gonad dissection. 📑 Their work using genetically encoded tags, an ex-vivo culture protocol, and the Vutara 352 allows for a new understanding of the dynamics of meiotic axis proteins required for meiosis. Full publication: https://lnkd.in/dp29An-m #Womeninstem #lifescience #microscopy

Don’t miss this exciting webcast, presented by Nature Research Custom Media, on how multimodal profiling can help you gain new insights about immune biology. #epigenomics #transcriptomics #spectralflowcytometry #ATACseq #BDRhapsody #BDBiosciences

Measuring GFP the right way! In the field of molecular biology, the use of Green Fluorescent Protein (GFP) has become a fundamental method for observing gene expression, the location of proteins within cells, and understanding how cells communicate through signaling pathways. GFP acts as a beacon, illuminating the inner workings of cells in a way that was previously impossible. The challenge, however, comes from autofluorescence - the phenomenon where cells and their components emit light naturally, without any external fluorescence markers like GFP. This natural glow can confuse the results, blending with the GFP signal and potentially leading to overestimations of GFP's presence. Our specially designed camera systems address this significant challenge head-on. Through the integration of advanced autofluorescence correction technologies, our cameras can distinguish the specific light emitted by GFP from the general glow of the cell. This capability is crucial for researchers who rely on precise and reliable fluorescence measurements to draw accurate conclusions from their experiments. Have you ever had this problem with GFP measurements? #horticulture #agritech #wageningen PhenoVation B.V.

🔬 𝗗𝗲𝗰𝗶𝗽𝗵𝗲𝗿𝗶𝗻𝗴 𝘁𝗵𝗲 𝗜𝗻𝘀𝗶𝗱𝗲 𝗖𝗲𝗹𝗹’𝘀 𝗦𝘁𝗼𝗿𝘆 𝘄𝗶𝘁𝗵 𝗖𝗬𝗧𝗢𝗖𝗛𝗘𝗖𝗞 𝗦𝗣𝗔𝗰𝗵𝗶𝗽®! 🌟 Exciting insights from 𝗔𝟰𝗹𝗶𝘃𝗲𝗖𝗲𝗹𝗹 𝗕𝗹𝗼𝗴 #𝟲! Discover how #SPAchip technology is revolutionizing genetic screening by mapping genotypes to phenotypes with image-based profiling. This advanced method captures intricate spatial and morphological changes in cells, offering a comprehensive view of genetic modifications’ effects. #SPAchip provides real-time readouts of metabolic activity, measuring key elements like pH, ROS, and Ca2+, enhancing research efficiency and accuracy.⁣ ⁣ Learn more about how #SPAchip® is advancing cellular biology and therapeutic research! 🚀🔍⁣ ⁣ ▶ Read the full blog here: https://lnkd.in/dgdX48Xd #SPAchip #GeneticScreening #LiveCellImaging #MetabolicActivity

Join Hye-Won Song and me as we share exciting data on the integration of spectral and image based flow cytometric cell sorting with downstream single cell ATAC-seq and whole transcriptome RNA seq

Are you a FISH, NGS or array newbie? Fear not fellow scientists, in our new series of educational posts we’ll explore some of the basics of these molecular genetic techniques 🧬 Today we answer the question what is FISH? Fluorescence in situ hybridization (FISH) uses fluorescently labeled DNA sequences (probes) to assess specific regions of interest, such as genes and centromeres, using fluorescence microscopy. The DNA sequence of the FISH probe is complementary to the region of interest. When using a FISH probe, if the region of interest is present, the probe will bind (hybridizes) to the target DNA sequence, which can then be visualized using fluorescence microscopy – indicating the presence of the gene, centromere, etc. If the region of interest has been deleted, the probe will not bind to the target DNA and so will not be visualized. A breakapart or a translocation FISH probe can be used to detect whether a gene has been rearranged. Below you can find a schematic of the FISH process. A more detailed explanation of this process is available on our website: https://lnkd.in/dnJSrR_T #fishbasics #CytoCell

🔬 Dive into the realm of dynamic structural biology with the latest methods primer on smFRET techniques. Explore the potential of smFRET for a range of applications, from genome maintenance and gene expression, to transmembrane transport and signalling. 💡 The comprehensive methods primer covers: - sample preparation including fluorophore selection - fluorescence microscopes for smFRET studies - data collection in vitro and in live cells - data analysis pipelines - limitations Emphasizing the importance of reproducibility and data transparency, the paper also discusses data deposition in citable open respositories. ➡ Read full text here: https://lnkd.in/euKYJKkf Do you want to incorporate smFRET into your own research toolbox? Perform easy and efficient smFRET experiments with Luminosa, the new single photon counting confocal microscope from PicoQuant. ➡ It`s user-friendly software includes context-based workflows for both diffusing and immobilized FRET samples, and even offers new flexibility with variable PSF feature: https://lnkd.in/ePSdNqzQ To keep analysis simple for you, the equations for single-molecule FRET corrections, which are based on a multi-laboratory benchmark study and discussed in the article are incorporated into the software and corrections are performed automatically in real-time. #Luminosa #smFRET #MicroTime #microscopy

🚸 Interesting article investigates meiotic recombination dynamics in plants with repeat-based holocentromeres, focusing on Rhynchospora breviuscula. 🚸 Meiotic recombination, initiated by DNA double-strand breaks, leads to the exchange of genetic material between homologous chromosomes, 🚸 Centromeres play a crucial role in genomic architecture and recombination dynamics, affecting crossover (CO) distribution. 🚸 Despite diffused centromeres in R. breviuscula, CO frequency shows a distal bias. 🚸 Crossovers are absent inside centromeric units but not in their proximity, suggesting a non-canonical centromere effect. 🚸 Telomere-led synapsis of homologues is proposed as the primary determinant of CO distribution. 🚸 The study highlights the importance of mechanistic features of meiotic pairing and synapsis over genomic features and centromere organization in shaping CO distribution. 🚸 The 'coarsening' model, involving HEI10 loading, fits observed CO patterns. 🚸 Telomeres harboring 35S rDNA exhibit low recombination frequencies due to late synapsis and HEI10 loading. 🚸 Haplotype-phased genomes and scRNA-seq enable investigation of CO frequencies in gametes. 🚸 While CO bias resembles other eukaryotes, correlation with (epi)genetic features is limited in R. breviuscula. 🚸 COs preferentially form within gene promoters, suggesting fine-scale regulation associated with open chromatin states. 🚸 The absence of a centromere effect is attributed to closed chromatin states of centromeric chromatin, indicating a potential strategy to address meiotic recombination in monocentric chromosomes. Like and share the knowledge :) Dr. Hamed Altaweel #meiosis #genetics #plants

🚀 Empowering Cellular Research with SPAchip Technology! 🚀 At the cutting edge of cellular biology, SPAchip technology is revolutionizing how we understand cellular metabolism. Through the innovative CytoCHECK assay, SPAchip provides real-time, non-invasive insights into the dynamic processes within living cells. A4CELL leads the charge with SPAchip, empowering scientists worldwide to unlock cellular secrets and drive forward research and therapeutic advancements. 👉 Dive deeper into the power of SPAchip and CytoCHECK on our latest blog post: Deciphering the Inside Cell’s Story by CytoCHECK SPAchip #CellularBiology #SPAchip #CytoCHECK #A4CELL #Biotech #Innovation #Research #CellMetabolism #RealTimeData