Rajanish Reddy Pallerla’s Post

Over the last few years, there has been a rapid increase in the demand for oligonucleotides production. Before being used in downstream applications, oligonucleotides must be cleaved from the solid support and have their protecting groups removed. Currently, although the synthesis process is automated and computer controlled, the post-processing steps involving cleavage and deprotection are still carried out manually. To address this challenge, 𝗠𝗶𝗹𝗲𝘀𝘁𝗼𝗻𝗲 𝗵𝗮𝘀 𝗱𝗲𝘃𝗲𝗹𝗼𝗽𝗲𝗱 𝘄𝗮𝘃𝗲𝗣𝗥𝗘𝗣, an efficient, rapid, and safe tool specifically designed for the post-processing 𝗰𝗹𝗲𝗮𝘃𝗮𝗴𝗲 𝗮𝗻𝗱 𝗱𝗲𝗽𝗿𝗼𝘁𝗲𝗰𝘁𝗶𝗼𝗻 (𝗖&𝗗) steps in oligonucleotide production. The wavePREP process combines cleavage and deprotection into a 𝘀𝗶𝗻𝗴𝗹𝗲 𝘀𝘁𝗲𝗽, eliminating the need for evaporation and ensuring a superior workflow with improved product yields. #waveprep #cleavage #deprotection #oligos #oligonucleotides #postsynthesis

Easy access to Quinazolinones and that too metal free via molecular iodine-mediated annulative coupling of sulfoxonium ylides with 2-aminobenzamide The method demonstrates remarkable chemoselectivity, broad substrate scope, scalability, and practical utility through diverse applications, including gram-scale reactions and the synthesis of biologically significant compounds including Tryptanthrin, bearing a fused indole quinazoline ring system. https://lnkd.in/gEFr5Nx8

Pure 15-cis-Phytoene, the biosynthetic precursor of all carotenoids, is now available at EXTRASYNTHESE as analytical standard. Check: https://lnkd.in/d8-sPDZ4

Quadrane sesquiterpenes featuring a distinctive tricyclic skeleton exhibit potent antimicrobial and anticancer activities. Although extensive studies have attempted to reveal the multistep carbocation rearrangement involved in the formation of the tricyclic quadrane scaffold, the exact biosynthetic pathway and chemical logic to generate the quadrane structure remains mysterious. In this week's #ChemSciPicks Yan Yan reports a novel sesquiterpene synthase that is capable of generating β-terrecyclene possessing the quadrane scaffold. They also provide insight into the mechanism that's involved. Read the full paper here: https://lnkd.in/eBz8PRqh

Greetings to all! I am thrilled to present our work on rarest category of Ru(III)-NHC complexes with abnormally bound carbene ligands in #RSCPoster under the division of #RSCInorg. Base free and simple synthesis of these benchtop stable kinetically accessible Ru(III)-aNHC complexes can be an interesting add-on in the organometallic chemistry of Ru complexes. Looking forward to Q/As in the comments. #RoyalSocChem

📢 Our 21st 𝒮𝒸𝒾𝑒𝓃𝓉𝒾𝒻𝒾𝒸 𝓁𝑒𝓉𝓉𝑒𝓇 by Jacques Lebreton is published on our website ! To read part II on Reduction with hydride reagents: an overview, click here ➡ https://lnkd.in/erg5ftWG Enjoy the reading #customsynthesis #smallmolecules

Another ClpP1P2 structures "electron density discovery" story, which is cute, but will undoubtedly be lost once this project makes it to publication. ClpP is a serine protease, and the project started by looking at boronate warheaded peptide substrate mimics (it moved quite far by now, into way more potent compounds, and away from boronates; that's why when it is published, this little vignette will be lost). Anyway, after building the inhibitor-Ser adduct and refining, some extra density was observed in every modified active site. The inhibitor was checked for integrity and identity, and it was puzzling: what could it be? After some literature study and reflection on the drops' composition, we realized that it is glycerol that made this cyclic ester, which is quite nicely refined into the extra density. I am not entirely sure what the little green tip peak is: too close to be coordinated water. I ended up deciding that it was from the second conformation of the ring... but never bothered to refine multiple states...

It's been a busy week for our lab - we have another preprint out on ChemRxiv! Here, we make progress toward our goal of establishing a unified synthetic strategy for accessing lots of different heterocycles. We take advantage of palladium's tendency to "walk" along carbon chains to enable cyclizations to larger ring sizes. Using our urea-enabled diene heteroannulation, we can now generate 5-, 6-, and 7-membered rings using a common synthetic route, just making a trivial change to one of the starting materials. Check out the awesome work by Owen Monteferrante, Kaitlyn Houghtling, and Aidan Kropiwnicki! https://lnkd.in/eYT45Ny9

A glimpse through the origin, composition and biomedical applications of green tea and its polyphenols: A review - https://smpl.is/9o2xe

Leverage the flexibility of the AdvanceBio oligonucleotide semi prep dimensions paired with an Agilent analytical instrument for easy column heating for optimal resolution. https://bit.ly/3VafVyE #AdvanceBio #oligonucleotide