We are excited to share with you some images from our lab of cell spheroids. Spheroids and organoids are culturing models which better imitate tumor and tissue in-vivo state, as compared to the traditional 2D monolayer cell culture. Spheroids exhibit the following features: 3D multicellular structure comprised by a network of cell-to-cell and cell-to-matrix interactions, similar to in-vivo tissue structure. Gene expressions which more closely resemble expression profiles of the respective tumor tissue in-vivo. Mimicking of tumor spatial organization - outer layer of highly proliferating cells, middle region comprising senescent cells and a core containing necrotic dead cells. Replicating the tumor microenvironment involves mirroring gradients of metabolites, oxygen, pH, and nutrients from the outer layer to the core. Enhanced complexity may be attained through co-culturing of tumor cells alongside other cell types from tumor microenvironment. It is now generally accepted that from physiological point of view, in many cases in comparison to mono layered culture, spheroids better represents solid tumor and it is advisable to use them for anti-cancer drug discovery and early stage development! #cell #spheroids #laboratory #3d
