Stratech Scientific Ltd.’s Post

Now available In Press and #OpenAccess: 'Bioreactor on a chip: A microfluidic device for closed production of human dendritic cells' from Kevin Loutherback, Peggy Bulur, and Allan Dietz. In this article, they share a novel platforming utilizing microfluidics to isolate, count, identify and culture cells in a closed, cGMP-compatible manner. The scalability and closed nature of the chip give it the potential to be a novel method used for cell therapy industrialization and rapid manufacturing. https://lnkd.in/gZzaKHaa

𝐀𝐫𝐞 𝐲𝐨𝐮 𝐬𝐜𝐫𝐞𝐞𝐧𝐢𝐧𝐠 𝐏𝐑𝐎𝐓𝐀𝐂𝐒 𝐚𝐧𝐝 𝐦𝐨𝐥𝐞𝐜𝐮𝐥𝐚𝐫 𝐠𝐥𝐮𝐞𝐬? Researchers worldwide use Tecan plate readers and dispensers to set up and screen their assays. Explore how you can measure NanoBRET, FP, HTRF, AlphaScreen, and cell viability—all with a single device. Read our Tecan Journal article on multiplexing PROTACs and molecular glues screening to learn more: https://lnkd.in/edAmhWzg

At Tecan, we stay ahead of new developments, always nurturing fresh insights in the rapidly evolving research field. We hope this new article sparks ( 😉 ) new ideas for your groundbreaking research. New Tecan Article Out Now 👇 #PROTAC #TPD #Biotech #DrugDiscovery #Tecan

Multiplexing Assay Readouts by Automated Fluorescence Microscopy Did you know that automated fluorescence microscopy can be used to multiplex several different assay readouts in a single experiment? That's why such assays are often called "high-content" assays, rather than "high-throughput" assays. But there is not really a tradeoff between high-content and high-throughput. A good drug-discovery assay can provide both. Consider the example shown in this post, in which viable cells and dead cells are stained in different fluorescent channels, and the size and shape of tumor spheroids are measured, all in response to different drug concentrations in 384-well format. For more insights into mastering cell-based imaging assays, check out our detailed white paper here:https://lnkd.in/gtNyBYY8 If you're interested in discussing a project involving cell-based imaging assays, Book a free discovery call with this link. https://lnkd.in/gRTuXWHD A Discovery Call is a perfect way for us to connect and have a great conversation to see which of our services are a good fit for you. Check out our website to explore the services we offer https://lnkd.in/gTQkdP2y Or contact info@scintillantbio.com #CellBasedAssays #DetectionTechnologies #BiochemicalAnalysis #MolecularEvents #CellularVisualization #BiochemicalQuantification #ResearchMethods #ExperimentalDesign #ScientificDiscovery #BiomedicalResearch #InnovativeTechnology #DataInsights #QuantitativeAnalysis #BenchtopScience #ResearchAdvancements #ScientificProgress #ExperimentalValidation #DiscoveryDriven #LaboratoryTechniques #CuttingEdgeScience

Do you know what's really in your sample? If you've been relying on traditional fluidic imaging cytometry, you’re likely missing the mark in distinguishing cells from other material. Enter Aura CL—bringing you accuracy with its Fluorescent Membrane Microscopy (FMM) technology. Now, identifying cellular aggregates in your cell therapy is a breeze. Download our app note to learn how to detect, ID, and characterize cellular vs. non-cellular aggregates. #particleanalysis #celltherapy

🔬 Explore the world of cell therapy at Takeda! Marvel at cardiac cells coalescing in a petri dish. 🧬 Discover ingenious CAR-T armoring with surface molecules for protection and augmentation. 🚀 Join us on the journey into the future of medical breakthroughs! Join Dr. Russell Weiner, Dr. Chad Briscoe, and Gregory Austin for Episode 4 of the BioTalk Unzipped Podcast which drops on January 15th. #CellTherapy #Biotechnology #MedicalAdvancements #drugdiscovery🌐

Innovative CalRexin® research in autophagy, “A novel pH-sensitive probe to quantify autophagy on high throughput/content imaging platforms” will be presented by Peter Wookey, Apop’s CSO, at Gordon Research Conferences. This exciting new research demonstrates the utility of CalRexin® pH-sensitive probe as a novel tool to image autophagy in live cell assays. Ground-breaking data on the utility of CalRexin® for primary high content screening of compounds that stimulate or inhibit autophagy!

Always great to be part of strides in science that open new possibilities.

Multiplexing in #singlecellproteomics can substantially increase throughput, but requires flexible and easy to implement, scalable and sensitive sample preparation strategies for highest data quality. In collaboration with the Slavov Lab, we demonstrate in this application note, an nPOP workflow that can accommodate different numbers of labels and different reagents simply by programming different configurations of droplets deposited on the surface of glass slides. nPOP with non-isobaric tags, or plexDIA approach, is providing an automated option for scaling up single-cell proteomics for analysis of thousands of single cells with proteome depths comparable to label-free DIA applications. We applied plexDIA in a fully automated 3-label approach on CFPAC-1 pancreatic cancer, WM-989 melanoma, and THP-1 leukemia cell lines (126 single cells in total) using the timsTOF Ultra in dia-PASEF mode. This approach allowed to analyze almost 100 samples per day with a proteome depth of more than 3000 proteins per cell (4500 in total) and quantification of > 80,000 precursors per individual plexDIA run. #TIMS separation aided in complexity reduction by MOMA. Intercalated MS1 scans in the dia-PASEF method increased the average number of data points per peak to 14.6 increasing quantitative accuracy. Read more: https://lnkd.in/euyegGUw #massspectrometry #proteomics #timsTOF

A pleasure to have been interviewed by Biocompare. Great summary of some of the key thinking in the fast-moving field of single cell science. #singlecell #microfluidics #singlecellanalysis #lightcast