The Structural Genomics Consortium (SGC)’s Post

A model discovered at the University of Victoria, Canada (using Transcreener assays) can accelerate the search for precise PI3Kα modulators that selectively modulate receptor interaction, membrane affinity, and/or ATPase function. https://lnkd.in/g-iB9bbe #drugdiscovery

A molecular dynamics study from the collaboration between AO Research Institute Davos (ARI) and Dalle Molle Institute for Artificial Intelligence ( USI Università della Svizzera italiana-Scuola universitaria professionale della Svizzera italiana (SUPSI) ) identified a possible physical interaction between the #Glucocorticoid #Receptor (GR) and #SOX9. This interaction appears modulated by #dexamethasone (DEX), making it more stable. ❓ We currently don't know what the function of this complex is, but SOX9 carries the GR coactivator motif LXXLL which is partially involved in the binding process. Also, we hypothesize that SOX9 can be "sequestered" by GR in the presence of DEX, preventing its dimerization and transcriptional activation of its targets - including SOX9 itself. This mechanism might explain at least partially why DEX induces SOX9 downregulation during in vitro #osteogenic differentiation of human bone marrow MSCs. 🧐 The implications of our findings can be manifold, and the upcoming steps are focused on unravelling the function of this complex. Read more at https://brnw.ch/21wGD2k

#BtBsPub: Antonino Natalello, Francesco Peri, Mohammed Monsoor Shaik, Alessio Romerio, Valentina Artusa, Diletta Ami, Juan Anguita, Sam Pasco and co-authors "Vibrational spectroscopy coupled with machine learning sheds light on the cellular effects induced by rationally designed TLR4 agonists." Talanta. 2024 Aug 1:275:126104. doi: 10.1016/j.talanta.2024.126104. In this work, we present the potential of Fourier transform infrared (FTIR) microspectroscopy to compare on whole cells, in an unbiased and untargeted way, the capacity of bacterial lipopolysaccharide (LPS) and two rationally designed molecules (FP20 and FP20Rha) to activate molecular circuits of innate immunity. These compounds are important drug hits in the... #BtBsUNIMIB , Università degli Studi di Milano-Bicocca https://lnkd.in/dc_HMn5n

Μøller, P., Azqueta, A., Boutet-Robinet, E. et al. Minimum Information for Reporting on the Comet Assay (MIRCA): recommendations for describing comet assay procedures and results. Nat Protoc 15, 3817–3826 (2020). ''The goal of the Minimum Information for Reporting Comet Assay (MIRCA) recommendations is to highlight key aspects of the comet assay procedure that must be described when reporting the results from cell culture studies, human biomonitoring and clinical studies''. https://lnkd.in/dYudtAdr

Synthesis and characterization of Pd (II) antitumor complex, DFT calculation and DNA/BSA binding insight through the combined experimental and theoretical aspects Mehran Feizi-Dehnayebi, E Dehghanian, H Mansouri-Torshizi Journal of Molecular Structure 1240, 130535 https://lnkd.in/d_-dzCXY

Nano-CUT&Tag for multimodal chromatin profiling at single-cell resolution Mapping of chromatin states at single-cell resolution is still challenging. This protocol describes nano-CT, a novel method to simultaneously characterize up to three epigenetic modalities at single-cell resolution. https://lnkd.in/ggPbFPna

Access the ultimate compilation of protocols for flow cytometry! The #flowcytometry Protocols Handbook will help you accelerate your research to help you plan and set up your flow cytometry experiments. The guide covers main procedural steps below to achieve your best results. Protocols included: ◾ Sample preparation ◾ #Immunecell stimulation ◾ #Phenotypic analysis ◾ Cell viability and apoptosis & #Cellcycle & Cell proliferation Download Now: https://lnkd.in/g6CvdWgr #pharmaindustry #biotechnologyindustry #research

Access the ultimate compilation of protocols for flow cytometry! The #flowcytometry Protocols Handbook will help you accelerate your research to help you plan and set up your flow cytometry experiments. The guide covers main procedural steps below to achieve your best results. Protocols included: ◾ Sample preparation ◾ #Immunecell stimulation ◾ #Phenotypic analysis ◾ Cell viability and apoptosis & #Cellcycle & Cell proliferation Download Now: https://lnkd.in/g6CvdWgr #pharmaindustry #biotechnologyindustry #research

Access the ultimate compilation of protocols for flow cytometry! The #flowcytometry Protocols Handbook will help you accelerate your research to help you plan and set up your flow cytometry experiments. The guide covers main procedural steps below to achieve your best results. Protocols included: ◾ Sample preparation ◾ #Immunecell stimulation ◾ #Phenotypic analysis ◾ Cell viability and apoptosis & #Cellcycle & Cell proliferation Download Now: https://lnkd.in/g6CvdWgr #pharmaindustry #biotechnologyindustry #research

#chirality #interface_chirality #bio_interfaces #biomedical_applications #drug_delivery #antibacterial #antiviral #tissue_repair #cell_fate #immunity #chiralpedia Interface Chirality: From Biological Effects to Biomedical Applications A brilliant review that focus on the latest discoveries and advancements in chiral bio-interfaces. It delves into how interface chirality influences cell fate, tissue repair, and immune responses in organisms. Biological applications of interface chirality, such as drug delivery, antibacterial, antiviral, and antitumor activities, as well as biological signal detection are explored. Further, the challenges and potential applications of chiral bio-interfaces in the biomedical field are also discussed. To summarize, this work examines the latest progress of chirality as regulator for controlling cell fate, tissue repair, and immune responses in organisms, along with its various biological applications. https://lnkd.in/gCFeBJeZ