Syncell Inc’s Post

📢Our latest application note has just been released! Discover the power of precision with Microscoop™ – the forefront of image-guided proteomics! 🎯 Our platform is redefining protein isolation by zeroing in on specific regions within cells and tissue samples. No guesswork here – just robust, hypothesis-free protein identification powered by microscopic analysis. 🔬 With Microscoop™, experience the power of precise photo-biotinylation utilizing ultrafast femtosecond pulses for two-photon illumination, pinpointing targets at the scale of individual organelles. This technology opens doors to a detailed exploration of subcellular protein dynamics and relationships, providing critical insights into the intricate subcellular structures that drive biological discovery. Learn more about the broad application of Microscoop in our new #AppNote - https://lnkd.in/gtz6_q6B #Microscoop #Proteomics #ProteinDiscovery #LaserNanoPulldown #ScoopBiology

We are going to publish a series of our App Notes covering Syncell's Microscoop technology and various applications showcasing our unique capability in subcellular spatial proteomic discovery. This is the first one briefing Microscoop methodology. #proteomics #spatialbiology #microscopy #omics

Hey everyone, ever feel like spatial biology analysis is a guessing game? 🤔 Say goodbye to the "known target" hassle and hello to hypothesis-free fun with MicroscoopTM! Imagine targeting specific region of interest in your samples, letting MicroscoopTM do its magic for automated, image-guided proteomic adventures. 🌟 No biases, just pure discovery! It's a real #spatialproteomics. And the best part? We're not just talking about high resolution—we're talking individual cellular organelle-level detail! 😲 I'm also excited to share that I am one of the authors. Check out our application note to delve deeper into the details of our magical system.

This interesting study provides insights into recent advancements in high-throughput microscopy for studying subcellular protein dynamics following perturbation. Reicher et al. introduce a new framework combining high-throughput microscopy, computer vision, and machine learning to detect changes in multicolour-tagged visual proteomics cell (vpCell) pools. By using genome-wide and cancer-focused sgRNA libraries, they created vpCell pools and an array of clones with unique fluorescent protein tags, enabling the simultaneous live-cell monitoring of numerous proteins. By analyzing localization patterns and expression levels of tagged proteins, they demonstrated the vpCell approach's capability to simultaneously track multiple proteins within a pathway. This method holds significant potential for mechanistic studies and drug discovery. Paper link: https://lnkd.in/dv_jfkTr #TiwariLab #WeeklyJournalClub #JournalClub #SDU

💡 Thank you Jay Nakamura Clark 🧠 from 🌟 Stellaromics Inc. 🌟 for connecting with me! It was so nice to learn about your company and your #SpatialBiology platform #STARmap (spatially-resolved transcript amplicon readout mapping). STARmap is #transcriptomic, but in 3D tissue blocks about 40-50 micron thickness. This integrates (1) hydrogel-tissue chemistry, (2) targeted signal amplification, and (3) in situ sequencing. So in essence, instead of z-stacking our images with thin slices, we can get a block of transcriptomic info in one run. The hydrogel also offers better stability for the sample. I'm sure 💙 Pathomics.ai would be very interested 🤔 in analyzing this data 📊 . You can read more about STARmap and 3-D intact-tissue sequencing in their Science paper. https://lnkd.in/gn7a-RtR

𝗛𝗶𝗴𝗵-𝗰𝗼𝗻𝘁𝗲𝗻𝘁 𝗰𝗲𝗹𝗹 𝗶𝗺𝗮𝗴𝗶𝗻𝗴 is crucial in drug discovery, enabling detailed analysis of cellular responses. Integrating seamlessly with genomics, proteomics, and computational biology, it enhances the precision and efficiency of progression through the discovery pipeline, 𝗺𝗮𝘅𝗶𝗺𝗶𝘇𝗶𝗻𝗴 𝗥&𝗗 𝗶𝗻𝘃𝗲𝘀𝘁𝗺𝗲𝗻𝘁𝘀. IRBM’s 𝗲𝘅𝗽𝗲𝗿𝘁𝗶𝘀𝗲 𝗶𝗻 𝗵𝗶𝗴𝗵-𝘁𝗵𝗿𝗼𝘂𝗴𝗵𝗽𝘂𝘁 𝗺𝗶𝗰𝗿𝗼𝘀𝗰𝗼𝗽𝘆 combined with the innovative imaging system of the Opera Phenix® Plus enables 𝗿𝗮𝗽𝗶𝗱 𝗶𝗱𝗲𝗻𝘁𝗶𝗳𝗶𝗰𝗮𝘁𝗶𝗼𝗻 𝗼𝗳 𝗹𝗲𝗮𝗱 𝗰𝗼𝗺𝗽𝗼𝘂𝗻𝗱𝘀 and the elucidation of their 𝗺𝗲𝗰𝗵𝗮𝗻𝗶𝘀𝗺𝘀 𝗼𝗳 𝗮𝗰𝘁𝗶𝗼𝗻. In addition to our ability to produce ad hoc, project-specific assays, some of our optimized assays include: // Antibody and Peptide Internalization // Spheroid Assays // Protein Localization // Neurite Outgrowth // Autophagy // Live Tracking // DNA Damage // Cell Cycle Analysis // Apoptosis and Cytotoxicity Supported by an expert team, high-content analysis software, and a 3D viewer, we can retrieve large amounts of data, perform complex analyses, and examine 3D cell models in both fixed and live cells, facilitating the interpretation of screening results and 𝗶𝗺𝗽𝗿𝗼𝘃𝗶𝗻𝗴 𝘁𝗵𝗲 𝗶𝗱𝗲𝗻𝘁𝗶𝗳𝗶𝗰𝗮𝘁𝗶𝗼𝗻 𝗼𝗳 𝗿𝗲𝗹𝗲𝘃𝗮𝗻𝘁 𝗽𝗵𝗲𝗻𝗼𝘁𝘆𝗽𝗲𝘀. Get in touch via the comments or a direct message to find out more about our cell imaging facility and how we can empower your drug discovery project. 📸 Our experts: Chiara Soldati, Valeria Colicchia, Sara Pugliese #cellimaging #highcontentscreening #drugdiscovery #IRBM

𝗔𝗽𝗽𝗹𝗶𝗰𝗮𝘁𝗶𝗼𝗻 𝗙𝗲𝗮𝘁𝘂𝗿𝗲: 𝗙𝘂𝗻𝗰𝘁𝗶𝗼𝗻𝗮𝗹 𝗟𝗶𝗴𝗵𝘁 𝗦𝗵𝗲𝗲𝘁 𝗖𝗮𝗹𝗰𝗶𝘂𝗺 𝗜𝗺𝗮𝗴𝗶𝗻𝗴 𝘄𝗶𝘁𝗵 𝗞𝗶𝗻𝗲𝘁𝗶𝘅 𝘀𝗖𝗠𝗢𝗦! The research was carried out by The Bianco Lab at UCL, aimed to understand the logic of brain circuits that control behaviour. Employing two-photon and light-sheet microscopy techniques alongside small, optically transparent larval zebrafish expressing genetically encoded calcium indicators (GECIs). It’s challenging to capture the dynamic signalling between neurons demands high speeds in calcium imaging. However, to effectively monitor populations of cells throughout the brain, it's essential to capture large imaging volumes. Consequently, the detector must possess capabilities for both high speed and a large field of view (FOV) to meet the demands of this high-throughput application. So, how did Kinetix sCMOS solve the case?  Click to find out more! For inquiries, contact us at sales@linx-sg.com or call: Singapore > +65 6842 0500 Malaysia > +60 3921 22099 Thailand > +66 2 082 1988 Article credit: Teledyne Photometrics, Customers Stories - https://lnkd.in/gTE-uR6N #scientific #CMOS #sCMOS #industrial #camera #lifescience #physicalscience #research #researchanddevelopment #medical #pharmaceutical 

❓ Question: What is Molecular Pixelation (MPX)? 💡 Answer: MPX is an optics-free, DNA sequence-based method for #spatial #proteomics of single cells using antibody-oligonucleotide conjugates (AOCs) and DNA-based, nanometer-sized molecular pixels. We want to highlight this relatively new #SpatialBiology platform from Pixelgen Technologies. MPX enables the co-localization of protein on the cell surface, can examine and designate polarity scores, or even allow for spatial analysis of chemotaxis. You can get more information from their latest #NatureMethods paper: https://lnkd.in/depdihwJ. The basis of this technology relies on #RollingCircleAmplification which we promise to highlight in the upcoming posts. So many Spatial Biology platforms rely on this simple replication method. Simon Fredriksson, William Love, Steve G., Trang Vu, PhD, Alex M., Trevor McKee

MALDI Imaging is a powerful mass spectrometry tool for mapping the distribution of molecules from a thin sample, ranging from small metabolites to large proteins, without molecular tags or labels. A single MALDI Imaging measurement produces up to several thousands of distribution maps, or ion images, to reveal greater insight and understanding of molecular makeup and regional heterogeneity. For more than 25 years, Bruker has been the leader in MALDI mass spectrometry innovation. MALDI is amenable for any analytical task and this is exemplified in the scientific literature where MALDI Imaging publications dominate 5:1 over the next ionization technology. Bruker delivers MALDI Imaging systems that incorporate novel MALDI technologies to accomplish any targeted or untargeted imaging study. This paper from Prof. Ron Heeren is a good example of what you can achieve with MSI using our timsTOF Flex. #bruker #massspectromery #imaging #msi #maldi #tissueimaging #spatialomics

Carbon dots (CDs) are gaining interest in medicine and pharmacy due to their stability, water dispersibility, and photoluminescence. CDs are explored for optical imaging, gene delivery, and theranostics. This report examines how Low Voltage Electron Microscopy (LVEM), particularly the LVEM 5, advances carbon dot research. https://bit.ly/3Vx5BzA