Syncell Inc’s Post

Hey everyone, ever feel like spatial biology analysis is a guessing game? 🤔 Say goodbye to the "known target" hassle and hello to hypothesis-free fun with MicroscoopTM! Imagine targeting specific region of interest in your samples, letting MicroscoopTM do its magic for automated, image-guided proteomic adventures. 🌟 No biases, just pure discovery! It's a real #spatialproteomics. And the best part? We're not just talking about high resolution—we're talking individual cellular organelle-level detail! 😲 I'm also excited to share that I am one of the authors. Check out our application note to delve deeper into the details of our magical system.

📢Our latest application note has just been released! Discover the power of precision with Microscoop™ – the forefront of image-guided proteomics! 🎯 Our platform is redefining protein isolation by zeroing in on specific regions within cells and tissue samples. No guesswork here – just robust, hypothesis-free protein identification powered by microscopic analysis. 🔬 With Microscoop™, experience the power of precise photo-biotinylation utilizing ultrafast femtosecond pulses for two-photon illumination, pinpointing targets at the scale of individual organelles. This technology opens doors to a detailed exploration of subcellular protein dynamics and relationships, providing critical insights into the intricate subcellular structures that drive biological discovery. Learn more about the broad application of Microscoop in our new #AppNote - https://lnkd.in/gtz6_q6B #Microscoop #Proteomics #ProteinDiscovery #LaserNanoPulldown #ScoopBiology

We are going to publish a series of our App Notes covering Syncell's Microscoop technology and various applications showcasing our unique capability in subcellular spatial proteomic discovery. This is the first one briefing Microscoop methodology. #proteomics #spatialbiology #microscopy #omics

Finally, after 9 years of work, the manuscript for our microscopic scooping technology is available on BioRxiv. Hopefully this technology can be broadly used to identify disease-related novel protein constituents at your microscopic regions of interest. #proteomics #spatialbiology #Syncell #Microscoop #neuroscience #cellbiology

Our new manuscript "Microscopy-guided subcellular proteomic discovery by high-speed ultra-content photo-biotinylation" is now accessible on bioRxiv! Microscopy-guided proteomics at an organelle-dimension resolution is desired for revealing unknown protein constituents at specific disease- or functional-associated regions at the molecular-molecular interactions level. Our innovative optoproteomics technology allows for precise spatial purification and identification of proteins within user-defined regions, automating the exploration of thousands of fields of view. Witness how our novel methodology has already identified stress granule-specific and amyloid β-localized proteins, confirmed through immunostaining. Check out the manuscript here:  https://lnkd.in/gMuKBWM9 #proteomics #spatialbiology #Syncell #Microscoop

❓ Question: What is Molecular Pixelation (MPX)? 💡 Answer: MPX is an optics-free, DNA sequence-based method for #spatial #proteomics of single cells using antibody-oligonucleotide conjugates (AOCs) and DNA-based, nanometer-sized molecular pixels. We want to highlight this relatively new #SpatialBiology platform from Pixelgen Technologies. MPX enables the co-localization of protein on the cell surface, can examine and designate polarity scores, or even allow for spatial analysis of chemotaxis. You can get more information from their latest #NatureMethods paper: https://lnkd.in/depdihwJ. The basis of this technology relies on #RollingCircleAmplification which we promise to highlight in the upcoming posts. So many Spatial Biology platforms rely on this simple replication method. Simon Fredriksson, William Love, Steve G., Trang Vu, PhD, Alex M., Trevor McKee

MALDI Imaging is a powerful mass spectrometry tool for mapping the distribution of molecules from a thin sample, ranging from small metabolites to large proteins, without molecular tags or labels. A single MALDI Imaging measurement produces up to several thousands of distribution maps, or ion images, to reveal greater insight and understanding of molecular makeup and regional heterogeneity. For more than 25 years, Bruker has been the leader in MALDI mass spectrometry innovation. MALDI is amenable for any analytical task and this is exemplified in the scientific literature where MALDI Imaging publications dominate 5:1 over the next ionization technology. Bruker delivers MALDI Imaging systems that incorporate novel MALDI technologies to accomplish any targeted or untargeted imaging study. This paper from Prof. Ron Heeren is a good example of what you can achieve with MSI using our timsTOF Flex. #bruker #massspectromery #imaging #msi #maldi #tissueimaging #spatialomics

💡 Thank you Jay Nakamura Clark 🧠 from 🌟 Stellaromics Inc. 🌟 for connecting with me! It was so nice to learn about your company and your #SpatialBiology platform #STARmap (spatially-resolved transcript amplicon readout mapping). STARmap is #transcriptomic, but in 3D tissue blocks about 40-50 micron thickness. This integrates (1) hydrogel-tissue chemistry, (2) targeted signal amplification, and (3) in situ sequencing. So in essence, instead of z-stacking our images with thin slices, we can get a block of transcriptomic info in one run. The hydrogel also offers better stability for the sample. I'm sure 💙 Pathomics.ai would be very interested 🤔 in analyzing this data 📊 . You can read more about STARmap and 3-D intact-tissue sequencing in their Science paper. https://lnkd.in/gn7a-RtR

This interesting study provides insights into recent advancements in high-throughput microscopy for studying subcellular protein dynamics following perturbation. Reicher et al. introduce a new framework combining high-throughput microscopy, computer vision, and machine learning to detect changes in multicolour-tagged visual proteomics cell (vpCell) pools. By using genome-wide and cancer-focused sgRNA libraries, they created vpCell pools and an array of clones with unique fluorescent protein tags, enabling the simultaneous live-cell monitoring of numerous proteins. By analyzing localization patterns and expression levels of tagged proteins, they demonstrated the vpCell approach's capability to simultaneously track multiple proteins within a pathway. This method holds significant potential for mechanistic studies and drug discovery. Paper link: https://lnkd.in/dv_jfkTr #TiwariLab #WeeklyJournalClub #JournalClub #SDU

Happy to share our latest research, with the release of our paper on bioRxiv. 📄🎉 In this work, we present an LA-ICP-TOFMS imaging pipeline that combines quantitative metal distribution at single-cell resolution with the identification of cellular phenotypes in tissues. As a showcase, an ex vivo human skin model exposed to cobalt chloride was investigated. This preprint is a snapshot of our work before peer review, so any feedback is welcome! #Metallomics #Bioimaging #DataAnalysis