Synthace’s Post

Transcript

Combine a Hamilton Star with the Synthase Digital experiment platform and enable easy, automated Dewey experiments executed end to end on a powerful liquid handler. We can plan out the steps of our experiment. Media optimization for neural stem cells. In the synthase no code workflow builder. Simply define the names of our media components. Tell Synthase what we want to do with them. And then repeat. Then we'll choose which components we want as DOE factors. And select an appropriate design. Before we've even entered the lab, Synthase will create an in silico preview of our experiment and generate a script for our Hamilton. Then with the click of a button we can send that script to the lab and press run. First, the Hamilton Star uses its eight channels to create the Dewey designed neural stem cell media mixtures in 96 well plates. After giving them a gentle mix, it then seeds GFP expressing cells in this media, using its 96 head to dispense into every well simultaneously using an adapted liquid class to minimize stress on cells. The execution is automatically paused, allowing us to incubate the cells for 48 hours. Before returning them to the Hamilton star. Synthese then communicates with another device, a biotech plate washer. And instructs the Hamilton to deliver plates to the washer so the neural cells can be washed. The plates are then returned to the Hamilton deck. And fixed with the power formaldehyde. After a few minutes and a quick wash. The cells are stained with Dappy to visualize their nuclei. After a final wash, Synthesis will communicate with the BMG Lab Tech plate reader. And instruct the Hamilton to deliver each plate to the reader for fluorescence based quantification of the proportion of cells expressing GFP. Data files from the plate reader are automatically uploaded into synthase. Allowing for easy analysis. Like making 3D surface blots. And optimizing your experiment. Hamilton and Synthase combining to enable powerful DOE experiments.