The Scientist’s Post

How to detect, count, and characterize particles in complex biological fluids such as unfiltered plasma, blood, and cell lysates? EOS Classizer™ ONE provides multiparametric calibration-free data on single particles based on their unique optical properties. Each particle population in a mixture of particle-particle or particle-biological corpuscles is classified, identified, and analyzed independently. Discover more about EOS Srl technologies at www.eosinstruments.com. (In the figure: aging of subvisible PLGA and PLGA+PEG particles for drug delivery incubated in unfiltered human plasma. EOS Classizer™ ONE enables the control of particle stability in real conditions to improve product formulation). #classizerone #eosinstruments #eosclouds #particleanalysis #particlesizing #particlecounting #spes #spestechnologies #nanoplastics

Turbulent mixers consistently produced lipid nanoparticles with a smaller average size, lower polydispersity and higher API encapsulation efficiency, highlighting the faster component mixing dynamics associated with turbulent mixers over microfluidics. 1. Faster component mixing leads to more homogenous particles. Why is this important? The above critical quality attributes have a great impact on biodistribution and efficacy of the API-LNP in vivo. Higher uniformity in the particles produced by turbulent mixers also has benefits in the process development and scaling up of these particles, as well as more consistent in vivo performance.  Learn more about it at Helix Biotech and our Nova LNP Manufacturing system https://lnkd.in/efdQTHHw The article: https://lnkd.in/eKSMbjfs #LNP #mRNALNP #NovaBT #HelixBiotech #Lipids #liposomes #turbulentmixers #lipidnanoparticles #RNAtherapeutics #drugdevelopment #drugdelivery

Interesting study comparing microfluidics to turbulent jet mixers for making lipid nanoparticles #LNPs. Interested to hear what you think on pros and cons for each technology. https://lnkd.in/dgnf5e6Z

An upcoming webinar from Advanced Materials Technology will present application examples demonstrating the impact of positively charged surface (PCS) stationary phases for both small molecules and peptides. In addition, the webinar will highlight speed and efficiency advantages of using such phases in combination with SPP columns. Register here: https://lnkd.in/dKzftYgT

Next-level #NanoParticle #Characterization: We are very happy to announce the launch of our latest #innovation, PhaSR-DLS! This cutting-edge addition to our NanoFlowSizer technology marks a significant advancement in nanoparticle size characterization. 🌟 What is PhaSR-DLS? PhaSR-DLS (Phase-sensitive Spatially Resolved Dynamic Light Scattering) takes our already powerful SR-DLS technology to the next level. By incorporating phase-sensitive detection, we can achieve: -Extended Application Range: At least a 20x reduction in the lowest accessible concentrations. -Higher Sensitivity: Ideal for weak scattering samples like proteins. -Enhanced Particle Size Distribution (PSD) accuracy and precision. -No Number Fluctuations: Delivering higher quality data of aggregates and large particles in suspension. 🌐 Key Benefits: -Versatility: Non-invasive measurements in various containers including syringes, IV-bags, and microfluidic applications. -Efficiency: Suitable for both inline Process Analytical Technology (PAT) and off-line applications. -Seamless Integration: Available as an add-on measurement mode to existing NFS instruments with no hardware modifications required. ✨Applications: PhaSR-DLS enhances the performance of NFS systems for detailed characterization in protein solutions, low-concentration liposome/LNP formulations, emulsions, and more. Whether for inline analysis of flowing suspensions or off-line nanoparticle synthesis and nano-milling processes, PhaSR-DLS provides unparalleled accuracy and precision. For more information, visit our PhaSR-DLS product page and download our comprehensive whitepaper: https://lnkd.in/eZ8U2fRE Join us in celebrating this advancement in nanoparticle characterization technology! #Innovation #Nanotechnology #ProductLaunch #InProcessLSP #PhaSRDLS #NanoparticleCharacterization #PAT

In high-sensitivity #proteomics, the primary sources of sample loss often occur during collection, preparation, and transfer.   To tackle these challenges, we’ve developed the proteoCHIP Evo96—a chip compatible with the Cellenion cellenONE instrument. This innovative platform integrates automated workflows, allowing direct peptide transfer into Evotips and eliminating the need for manual handling.   Key features include:  • High throughput  • Precision isolation  • Enhanced sensitivity  • Streamlined workflow   The proteoCHIP Evo96 is the result of combining our liquid chromatography expertise with Cellenion’s state-of-the-art single-cell isolation technology. This platform enables high-throughput single-cell proteomics analysis with unprecedented precision, sensitivity, and efficiency.   With this advancement, you can explore cellular heterogeneity with great accuracy and confidence.   Learn more about automated, simple, seamless sample preparation here: https://lnkd.in/eid4Y84J

An excellent resource for visualising the effect on electrostatics when applying the Topliss tree.

𝐓𝐫𝐚𝐧𝐬𝐟𝐨𝐫𝐦𝐢𝐧𝐠 𝐒𝐚𝐦𝐩𝐥𝐞 𝐇𝐚𝐧𝐝𝐥𝐢𝐧𝐠 𝐢𝐧 𝐅𝐥𝐨𝐰 𝐂𝐲𝐭𝐨𝐦𝐞𝐭𝐫𝐲 Flow cytometry, a powerful technique for analyzing and quantifying cellular characteristics, has become indispensable in fields ranging from biomedical research to clinical diagnostics. Achieving pulsation-free sheath fluid movement for flow cytometry has been a challenge previously met only by traditional gear or peristaltic pumps. Thanks to their innovative Smooth Flow Technology for gentle and precise liquid handling, #KNF diaphragm pumps are driving innovation by offering a reliable, state-of-the-art solution that is revolutionizing cell sample transport in flow cytometry systems. Learn more about how our innovative Smooth Flow Technology can improve flow cytometry performance: #FlowCytometry #Research #Precision #SmoothFlow

The SPLPermea™ Dish ensures optimal gas exchange for effective cell culture. Due to its low water permeability, the culture medium is maintained for a long time without leakage or evaporation. The SPLPermea™ Dish has low auto-fluorescence with high light transmission to enable applications in imaging and micro-reading • Ergonomic design for easy handling and minimal contamination  • Gas-permeability tested (CO2, O2) • Leakage and evaporation tested • Transparent for microscopic observation and imaging • Low auto-fluorescence • High light transmission • Sterile ▪ Non-cytotoxic ▪ Non-pyrogenic ▪ DNase / RNase – free ▪ Human DNA – free Learn more : spllifesciences.com Product link : https://lnkd.in/gEV_bdKk #SPL #spllifesciences #lifesciences #biotechnology #labware #labproduct #laboratory #Permea #Dish #CO2 #Cell #Cellculture

MALDI analysis of polymers does not have to be hard. MALDI is a fast technique that can help you confirm the polydispersity, end groups, track stability and identity of your polymer samples with minimal fuss. In this webinar, I explain the basics of the MALDI technique and walk through sample preparation through to data analysis on the MALDI-8030 benchtop MALDI TOF mass spectrometer. Please get in touch with me, Michael Nairn PhD, or Christine Hinz if you would like to know more. #MALDI #Benchtop #Polymer #characterisation #MALDI8020 #MALDI8030 #pegylated