Tresa Elias’ Post

Tip convolution? Or surface structure? 🔬 It’s well known in the world of scanning probe microscopy (SPM) that SPM measurements are a convolution of the probe tip and the surface (which is a convolution of the surface material, and the surface preparation steps, etc. – but we won’t get into that now…) Imaging repeating patterns is one of the tell-tale signs for a blunt probe tip. These artifacts appear because the shape of the tip is being imaged, rather than the shape of the surface structures. If a pyramidal shaped tip is used, these patterns appear as triangles! 🔺 ❔ Is that what we can see in the data shown below? Not this time! The image below is real data – the tip is perfectly sharp. This map (16 um by 5.7 um with a z range of 85 nm) was collected from a sample of quartz that has been acid etched, resulting in numerous triangular shaped pits. Here at BND, we regularly use samples such as this to assess stage linearity and to further develop our large area stitching capabilities. If you would like to know more about our technology, including our latest model of Dynamic-SPM: Vector, check out our website (https://lnkd.in/eAJjjCvh) or drop us a LinkedIn message 💬 - we would love to hear from you! #Nanoscale #Nanotechnology #ScanningProbeMicroscopy #Microscopy #MeasurementServices #BristolNanoDynamics

Webinar: Combining Fluorescence Microscopy and 3D reconstruction to investigate neuron-glia interaction in different neurological diseases

Publications alert 📢!! 1) The influence of dislocations on B19' and R-phase transformations in a NiTi shape memory alloy. [Scripta Materialia, link: https://lnkd.in/gCesx7zY] 2) A micromechanical study of heat treatment induced hardening in α-brass. [Acta Materialia, link: https://lnkd.in/gJvFeG3W] 3) Microscale stress-geometry interactions in an additively manufactured NiTi cardiovascular stent: A synchrotron dual imaging tomography and diffraction study. [Materials Characterization, link: https://lnkd.in/gBterYTD]

Do you have bleed-through in your multichannel fluorescence microscopy images? Try out PICASSO-Lite, the algorithm I developed for blind, n-channel spectral unmixing. Please feel free to comment on the article or email me with your thoughts and I will address them in future updates!

DriveAFM is the premier AFM for life science research: its unbeaten versatility allows you to examine a wide range of samples, from single molecules to cells, all with a single AFM platform. DriveAFM combines high resolution imaging with a large scan range, providing the measurement flexibility you need, and allows you to combine AFM with other techniques that will help you understand your sample. https://hubs.ly/Q02dsNWy0

White light diffraction phase microscopy for imaging of red blood cells for different storage times https://lnkd.in/dBcjmKux

Full spectral detection from 400 to 900 with the highest efficiency and the lowest background noise. And of course counting photons for reliable quantification on confocal imaging. T R A N S F O R M I N G P R E C I S I O N I M A G I N G 🔬🦋

Some basics of confocal, SIM and STED microscopy

In this study, regular low-spatial-frequency LIPSS (LSFL) with a period of 760 nm was fabricated in advance on a silicon surface with two-beam interference using an 800 nm, 50 fs femtosecond laser. The ultrafast dynamics of high-spatial-frequency LIPSS (HSFL) formation on the silicon surface of prefabricated LSFL under single femtosecond laser pulse irradiation were observed and analyzed for the first time using collinear pump-probe imaging method. Full-length paper available at https://lnkd.in/gTGkeRY3 Optical scanning endoscope via a single multimode optical fiber

Do you want to create high-quality Raman images, quickly? In our next webinar focused solely on fast Raman imaging, you’ll learn all about fast Raman imaging methods, how to optimise them, as well as how to process and analyse data to get meaningful results. Here's an example of how Raman imaging can reveal chemistry that isn't visible by optical microscopy. Register here: https://bit.ly/4b7m9E3